Combinatorial Tuning of Multigene Biosynthetic Pathways based on RNA Interference. [PDF]
Adv Sci (Weinh)Small RNA sequencing identifies candidate endogenous Pol III promoters, and subsequent fluorescence screening measures knockdown efficiency to assess promoter strength. Combining promoter strength and shRNA target position enables fine‐grained RNAi regulation of multigene pathways. This strategy enhances β‐carotene production in Schizochytrium sp.
Zhang Z +6 more
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Bacterial Delivery of RNAi Effectors: Transkingdom RNAi [PDF]
Journal of Visualized Experiments, 2010 RNA interference (RNAi) represents a high effective mechanism for specific inhibition of mRNA expression. Besides its potential as a powerful laboratory tool, the RNAi pathway appears to be promising for therapeutic utilization. For development of RNA interference (RNAi)-based therapies, delivery of RNAi-mediating agents to target cells is one of the ...
Lage, Hermann, Krühn, Andrea
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Asian Citrus Psyllid RNAi Pathway – RNAi evidence [PDF]
Scientific Reports, 2016 Abstract Diaphorina citri , known as the Asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease).
Taning, Nji Tizi Clauvis +4 more
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RNAi in Clinical Studies [PDF]
Current Medicinal Chemistry, 2013 RNA interference (RNAi) is an efficient process of posttranscriptional gene silencing. In recent years it has been developed into a new technology in biopharmaceutical fields of science. RNAi products include short interference RNA (siRNA) but also short hairpin RNA (shRNA), bifunctional short hairpin RNA (bi-shRNA) and microRNA (miRNA).
Kubowicz, P. +2 more
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Tumor inhibition by genomically integrated inducible RNAi-cassettes [PDF]
, 2006 RNA interference (RNAi) has emerged as a powerful tool to induce loss-of-function phenotypes by post-transcriptional silencing of gene expression. In this study we wondered whether inducible RNAi-cassettes integrated into cellular DNA possess the power ...
Kappel, Sven +8 more
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Frontiers in RNAi: Volume 1 [PDF]
, 2014 RNA interference (RNAi) is a biological process in which small nucleotide sequences destroy specific mRNA molecules and, therefore, interrupt genetic expression.
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Evidence of a tick RNAi pathway by comparative genomics and reverse genetics screen of targets with known loss-of-function phenotypes in Drosophila [PDF]
, 2009 Background The Arthropods are a diverse group of organisms including Chelicerata (ticks, mites, spiders), Crustacea (crabs, shrimps), and Insecta (flies, mosquitoes, beetles, silkworm).
Rodriguez Valle, Manuel +35 more
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Targeted transgenic RNAi knockdown of cell fate determinants induces neoplastic tumor growth and metastasis in a Drosophila transplantation model of neural stem cell derived cancer [PDF]
, 2012 Genetic models such as Drosophila have sophisticated transgenic and molecular genetic tools available to investigate proliferation control in normal and tumorigenic neural stem cells.
Reichert, Heinrich +3 more
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RNA-Based Therapeutics: From Antisense Oligonucleotides to miRNAs
Cells, 2020 The first therapeutic nucleic acid, a DNA oligonucleotide, was approved for clinical use in 1998. Twenty years later, in 2018, the first therapeutic RNA-based oligonucleotide was United States Food and Drug Administration (FDA) approved. This promises to
Sarah Bajan, Gyorgy Hutvagner
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Fission yeast Dicer harbours a unique dsRBD that participates in the spatial organization of the RNAi pathway [PDF]
, 2011 The term RNAi describes a set of conserved pathways found in most eukaryotes. RNAi is involved in various cellular processes, ranging from the control of gene expression to the establishment of heterochromatic structures.
Emmerth, Stephan
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