RNase H and Its Effects on PCR [PDF]
BioTechniques, 2002 Swamy K. Polumuri +2 more
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RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes [PDF]
Bio-Protocol, 2015 The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA (Figure ...
Angela Corona, Enzo Tramontano
doaj +2 more sources
Structure-Based Design of Novel Thiazolone[3,2-a]pyrimidine Derivatives as Potent RNase H Inhibitors for HIV Therapy [PDF]
MoleculesRibonuclease H (RNase H) was identified as an important target for HIV therapy. Currently, no RNase H inhibitors have reached clinical status. Herein, a series of novel thiazolone[3,2-a]pyrimidine-containing RNase H inhibitors were developed, based on ...
Xuan-De Zhu +8 more
doaj +2 more sources
Design, Synthesis and Biological Evaluation of 3-Hydrazonoindolin-2-one Derivatives as Novel HIV-1 RNase H Inhibitors [PDF]
MoleculesTargeting ribonuclease H (RNase H) has emerged as a highly promising strategy for treating HIV-1. In this study, a series of novel 3-hydrazonoindolin-2-one derivatives were designed and synthesized as potential inhibitors of HIV-1 RNase H.
Yiying Zhang +10 more
doaj +2 more sources
Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier [PDF]
Frontiers in PharmacologyRibonuclease H (RNase H), a critical functional protein in replication and genome stability, is emerging as a crucial therapeutic target for various diseases, including immune disorders.
Sheng Ding +6 more
doaj +2 more sources
Retroviral RNase H: Structure, mechanism, and inhibition. [PDF]
Enzymes, 2021 All retroviruses encode the enzyme, reverse transcriptase (RT), which is involved in the conversion of the single-stranded viral RNA genome into double-stranded DNA. RT is a multifunctional enzyme and exhibits DNA polymerase and ribonuclease H (RNH) activities, both of which are essential to the reverse-transcription process.
Ilina TV +3 more
europepmc +4 more sources
Crystal structure of archaeal RNase HII: a homologue of human major RNase H [PDF]
Structure, 2000 RNases H are present in all organisms and cleave RNAs in RNA/DNA hybrids. There are two major types of RNases H that have little similarity in sequence, size and specificity. The structure of RNase HI, the smaller enzyme and most abundant in bacteria, has been extensively studied.
Luhua Lai +4 more
openalex +5 more sources
Flavonoids and Acid-Hydrolysis derivatives of Neo-Clerodane diterpenes from Teucrium flavum subsp. glaucum as inhibitors of the HIV-1 reverse transcriptase–associated RNase H function [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2021 Bioassay-guided fractionation of the ethyl acetate extract from Teucrium flavum subsp. glaucum, endowed with inhibitory activity towards the HIV-1 reverse transcriptase–associated RNase H function, led to the isolation of salvigenin (1), cirsimaritin (2)
Benedetta Fois +8 more
doaj +2 more sources
Targeting HIV-1 RNase H: N’-(2-Hydroxy-benzylidene)-3,4,5-Trihydroxybenzoylhydrazone as Selective Inhibitor Active against NNRTIs-Resistant Variants [PDF]
Viruses, 2020 HIV-1 infection requires life-long treatment and with 2.1 million new infections/year, faces the challenge of an increased rate of transmitted drug-resistant mutations.
Angela Corona +12 more
doaj +2 more sources
Characterization of Echinostoma cinetorchis endoribonuclease, RNase H. [PDF]
Korean J Parasitol, 2017 Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters.
Lim SB +9 more
europepmc +4 more sources