Results 1 to 10 of about 290,253 (274)

Targeting HIV-1 RNase H: N’-(2-Hydroxy-benzylidene)-3,4,5-Trihydroxybenzoylhydrazone as Selective Inhibitor Active against NNRTIs-Resistant Variants [PDF]

Viruses, 2020
HIV-1 infection requires life-long treatment and with 2.1 million new infections/year, faces the challenge of an increased rate of transmitted drug-resistant mutations.
Angela Corona, Ester Ballana, Simona Distinto, Dominga Rogolino, Claudia Del Vecchio, Mauro Carcelli, Roger Badia, Eva Riveira-Muñoz, Francesca Esposito, Cristina Parolin, José A. Esté, Nicole Grandi, Enzo Tramontano   +12 more
doaj   +4 more sources

Analysis and Molecular Determinants of HIV RNase H Cleavage Specificity at the PPT/U3 Junction [PDF]

Viruses, 2021
HIV reverse transcriptases (RTs) convert viral genomic RNA into double-stranded DNA. During reverse transcription, polypurine tracts (PPTs) resilient to RNase H cleavage are used as primers for plus-strand DNA synthesis.
Mar Álvarez, Enrique Sapena-Ventura, Joanna Luczkowiak, Samara Martín-Alonso, Luis Menéndez-Arias   +4 more
doaj   +4 more sources

Retroviral RNase H: Structure, mechanism, and inhibition. [PDF]

Enzymes, 2021
All retroviruses encode the enzyme, reverse transcriptase (RT), which is involved in the conversion of the single-stranded viral RNA genome into double-stranded DNA. RT is a multifunctional enzyme and exhibits DNA polymerase and ribonuclease H (RNH) activities, both of which are essential to the reverse-transcription process.
Ilina TV, Brosenitsch T, Sluis-Cremer N, Ishima R.   +3 more
europepmc   +3 more sources

Structure-Based Design of Novel Thiazolone[3,2-a]pyrimidine Derivatives as Potent RNase H Inhibitors for HIV Therapy [PDF]

Molecules
Ribonuclease H (RNase H) was identified as an important target for HIV therapy. Currently, no RNase H inhibitors have reached clinical status. Herein, a series of novel thiazolone[3,2-a]pyrimidine-containing RNase H inhibitors were developed, based on ...
Xuan-De Zhu, Angela Corona, Stefania Maloccu, Enzo Tramontano, Shuai Wang, Christophe Pannecouque, Erik De Clercq, Ge Meng, Fen-Er Chen   +8 more
doaj   +2 more sources

Design, Synthesis and Biological Evaluation of 3-Hydrazonoindolin-2-one Derivatives as Novel HIV-1 RNase H Inhibitors [PDF]

Molecules
Targeting ribonuclease H (RNase H) has emerged as a highly promising strategy for treating HIV-1. In this study, a series of novel 3-hydrazonoindolin-2-one derivatives were designed and synthesized as potential inhibitors of HIV-1 RNase H.
Yiying Zhang, Rao Wang, Yueyue Bu, Angela Corona, Laura Dettori, Enzo Tramontano, Christophe Pannecouque, Erik De Clercq, Shuai Wang, Ge Meng, Fen-Er Chen   +10 more
doaj   +2 more sources

Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier [PDF]

Frontiers in Pharmacology
Ribonuclease H (RNase H), a critical functional protein in replication and genome stability, is emerging as a crucial therapeutic target for various diseases, including immune disorders.
Sheng Ding, Yinghua Wei, Minglong Yang, Jinyi Shi, Kaiyuan Ren, Xinli Li, Zhuo Tang   +6 more
doaj   +2 more sources

Flavonoids and Acid-Hydrolysis derivatives of Neo-Clerodane diterpenes from Teucrium flavum subsp. glaucum as inhibitors of the HIV-1 reverse transcriptase–associated RNase H function [PDF]

Journal of Enzyme Inhibition and Medicinal Chemistry, 2021
Bioassay-guided fractionation of the ethyl acetate extract from Teucrium flavum subsp. glaucum, endowed with inhibitory activity towards the HIV-1 reverse transcriptase–associated RNase H function, led to the isolation of salvigenin (1), cirsimaritin (2)
Benedetta Fois, Angela Corona, Enzo Tramontano, Simona Distinto, Elias Maccioni, Rita Meleddu, Pierluigi Caboni, Costantino Floris, Filippo Cottiglia   +8 more
doaj   +2 more sources

RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes

Bio-Protocol, 2015
The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA (Figure ...
Angela Corona, Enzo Tramontano
doaj   +2 more sources

An RNase H‐Like gene complements resistance to Bean common mosaic necrosis virus in Phaseolus vulgaris [PDF]

The Plant Genome
Bean common mosaic virus and Bean common mosaic necrosis virus (BCMNV) are related positive‐sense RNA potyviruses that limit the production of common bean (Phaseolus vulgaris L.) worldwide.
Alvaro Soler‐Garzón, Phillip N. Miklas   +1 more
doaj   +2 more sources

Characterization of Echinostoma cinetorchis endoribonuclease, RNase H. [PDF]

Korean J Parasitol, 2017
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters.
Lim SB, Cha SH, Jegal S, Jun H, Park SH, Jeon BY, Pak JH, Bakh YY, Kim TS, Lee HW.   +9 more
europepmc   +4 more sources