Structure-Based Design of Novel Thiazolone[3,2-a]pyrimidine Derivatives as Potent RNase H Inhibitors for HIV Therapy [PDF]
MoleculesRibonuclease H (RNase H) was identified as an important target for HIV therapy. Currently, no RNase H inhibitors have reached clinical status. Herein, a series of novel thiazolone[3,2-a]pyrimidine-containing RNase H inhibitors were developed, based on ...
Xuan-De Zhu +8 more
doaj +5 more sources
RNase H As Gene Modifier, Driver of Evolution and Antiviral Defense [PDF]
Frontiers in Microbiology, 2017 Retroviral infections are ‘mini-symbiotic’ events supplying recipient cells with sequences for viral replication, including the reverse transcriptase (RT) and ribonuclease H (RNase H).
Karin Moelling +4 more
doaj +3 more sources
RNase H-based analysis of synthetic mRNA 5' cap incorporation. [PDF]
RNA, 2022 Advances in mRNA synthesis and lipid nanoparticles technologies have helped make mRNA therapeutics and vaccines a reality. The 5’ cap structure is a crucial modification required to functionalize synthetic mRNA for efficient protein translation in vivo ...
Chan SH +7 more
europepmc +2 more sources
The atlas of RNase H antisense oligonucleotide distribution and activity in the CNS of rodents and non-human primates following central administration. [PDF]
Nucleic Acids Res, 2021 Antisense oligonucleotides (ASOs) have emerged as a new class of drugs to treat a wide range of diseases, including neurological indications. Spinraza, an ASO that modulates splicing of SMN2 RNA, has shown profound disease modifying effects in Spinal ...
Jafar-Nejad P +13 more
europepmc +2 more sources
RNase HI Is Essential for Survival of Mycobacterium smegmatis. [PDF]
PLoS ONE, 2015 RNases H are involved in the removal of RNA from RNA/DNA hybrids. Type I RNases H are thought to recognize and cleave the RNA/DNA duplex when at least four ribonucleotides are present.
Alina E Minias +7 more
doaj +17 more sources
RNase H enables efficient repair of R-loop induced DNA damage. [PDF]
bioRxiv, 2016 R-loops, three-stranded structures that form when transcripts hybridize to chromosomal DNA, are potent agents of genome instability. This instability has been explained by the ability of R-loops to induce DNA damage. Here, we show that persistent R-loops
Amon, Jeremy D, Koshland, Douglas
core +2 more sources
Insights into the structure and activity of prototype foamy virus RNase H [PDF]
Retrovirology, 2012 Background RNase H is an endonuclease that hydrolyzes the RNA strand in RNA/DNA hybrids. Retroviral reverse transcriptases harbor a C-terminal RNase H domain whose activity is essential for viral replication.
Leo Berit +4 more
doaj +4 more sources
Design, Synthesis and Biological Evaluation of 3-Hydrazonoindolin-2-one Derivatives as Novel HIV-1 RNase H Inhibitors [PDF]
MoleculesTargeting ribonuclease H (RNase H) has emerged as a highly promising strategy for treating HIV-1. In this study, a series of novel 3-hydrazonoindolin-2-one derivatives were designed and synthesized as potential inhibitors of HIV-1 RNase H.
Yiying Zhang +10 more
doaj +2 more sources
Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier [PDF]
Frontiers in PharmacologyRibonuclease H (RNase H), a critical functional protein in replication and genome stability, is emerging as a crucial therapeutic target for various diseases, including immune disorders.
Sheng Ding +6 more
doaj +2 more sources
Ribonuclease H/DNA Polymerase HIV-1 Reverse Transcriptase Dual Inhibitor: Mechanistic Studies on the Allosteric Mode of Action of Isatin-Based Compound RMNC6. [PDF]
PLoS ONE, 2016 The DNA polymerase and ribonuclease H (RNase H) activities of human immunodeficiency virus type 1 (HIV-1) are needed for the replication of the viral genome and are validated drug targets.
Angela Corona +10 more
doaj +7 more sources