Results 11 to 20 of about 303,152 (296)
Crystal structure of archaeal RNase HII: a homologue of human major RNase H [PDF]
Structure, 2000 RNases H are present in all organisms and cleave RNAs in RNA/DNA hybrids. There are two major types of RNases H that have little similarity in sequence, size and specificity. The structure of RNase HI, the smaller enzyme and most abundant in bacteria, has been extensively studied.
Lai, Luhua +4 more
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Insights into the structure and activity of prototype foamy virus RNase H
Retrovirology, 2012 Background RNase H is an endonuclease that hydrolyzes the RNA strand in RNA/DNA hybrids. Retroviral reverse transcriptases harbor a C-terminal RNase H domain whose activity is essential for viral replication.
Leo Berit +4 more
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Virology, 2001 Escherichia coli RNase H has a basic extension that is involved in binding nucleic acid substrates. This basic extension is present in the RNase H of Moloney murine leukemia virus reverse transcriptase (MLV RT), but has been deleted from the RNase H of HIV-1 RT. Previous work showed that removing the basic loop from MLV RT (the mutant is called DeltaC)
Boyer, Paul L. +4 more
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Cycling Probe Technology with RNase H Attached to an Oligonucleotide
BioTechniques, 1996 A streptavidin-RNase H gene fusion was constructed by cloning the Thermus thermophilus RNase H coding sequence in the streptavidin expression vector pTSA18F.
F. Bekkaoui +4 more
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Detection of an RNase H activity associated with hepadnaviruses
Journal of Virology, 1995 Replication of the hepadnavirus DNA genome is accomplished via reverse transcription of an intermediate, pregenomic RNA molecule. This process is likely to be carried out by a virally encoded, multifunctional polymerase which possesses DNA- and RNA-dependent DNA polymerase and RNase H activities.
S M, Oberhaus, J E, Newbold
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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H. [PDF]
Korean J Parasitol, 2017 Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters.
Lim SB +9 more
europepmc +4 more sources
N-Hydroxypiridinedione: A Privileged Heterocycle for Targeting the HBV RNase H
MoleculesHepatitis B virus (HBV) remains a global health threat. Ribonuclease H (RNase H), part of the virus polymerase protein, cleaves the pgRNA template during viral genome replication.
Dimitrios Moianos +8 more
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RNase H and Its Effects on PCR [PDF]
BioTechniques, 2002 Swamy K. Polumuri +2 more
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RNase H As Gene Modifier, Driver of Evolution and Antiviral Defense [PDF]
Frontiers in Microbiology, 2017 Retroviral infections are ‘mini-symbiotic’ events supplying recipient cells with sequences for viral replication, including the reverse transcriptase (RT) and ribonuclease H (RNase H).
Karin Moelling +4 more
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Synthesis, biophysical properties, and RNase H activity of 6'-difluoro[4.3.0]bicyclo-DNA. [PDF]
Beilstein J Org Chem, 2019 Here we present the synthesis, the biophysical properties, and the RNase H profile of 6’-difluorinated [4.3.0]bicyclo-DNA (6’-diF-bc4,3-DNA). The difluorinated thymidine phosphoramidite building block was synthesized starting from an already known gem ...
Frei S, Katolik AK, Leumann CJ.
europepmc +2 more sources