Results 11 to 20 of about 11,506,565 (250)

The Role of Conserved Residues in the DEDDh Motif: the Proton-Transfer Mechanism of HIV-1 RNase H [PDF]

, 2021
RNase H is a prototypical example for two-metal-ion catalysis in enzymes. An RNase H activity cleaving the ribonucleic acid (RNA) backbone of a DNA/RNA hybrid is present not only in important drug targets, such as the HIV-1 reverse transcriptase, but ...
Duerr, Simon L., Shao, Y, Denes Berta, Peter, C, Jambrina, PG, Rosta, Edina, Berta, D, Rosta, E, Dürr, SL, Dürr, Simon, Yihan Shao, Peter, Christine, García Jambrina, Pablo, Suardiaz, R, Reynier Suardiaz, Dürr, Simon L., Suardiaz, Reynier, Suardíaz Delrío, Reynier, Jambrina, Pablo, Dürr, Simon Leonard, Berta, Denes, Bohuszewicz, Olga, Bohuszewicz, O, Pablo Garcia Jambrina, Jambrina, Pablo G., Christine Peter, Olga Bohuszewicz, Edina Rosta, Simon L. Dürr, Shao, Yihan   +29 more
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Acetylation regulates the oligomerization state and activity of RNase J, the Helicobacter pylori major ribonuclease

Nature Communications, 2023
In the gastric pathogen Helicobacter pylori, post-transcriptional regulation relies strongly on the activity of the essential ribonuclease RNase J. Here, we elucidated the crystal and cryo-EM structures of RNase J and determined that it assembles into ...
Alejandro Tejada-Arranz, Aleksei Lulla, Maxime Bouilloux-Lafont, Evelyne Turlin, Xue-Yuan Pei, Thibaut Douché, Mariette Matondo, Allison H. Williams, Bertrand Raynal, Ben F. Luisi, Hilde De Reuse   +10 more
doaj   +1 more source

2-(Arylamino)-6-(trifluoromethyl)nicotinic Acid Derivatives: New HIV-1 RT Dual Inhibitors Active on Viral Replication

Molecules, 2020
The persistence of the AIDS epidemic, and the life-long treatment required, indicate the constant need of novel HIV-1 inhibitors. In this scenario the HIV-1 Reverse Transcriptase (RT)-associated ribonuclease H (RNase H) function is a promising drug ...
Angela Corona, Valentina Onnis, Claudia Del Vecchio, Francesca Esposito, Yung-Chi Cheng, Enzo Tramontano   +5 more
doaj   +1 more source

The catalytic mechanism, metal dependence, substrate specificity, and biodiversity of ribonuclease H

Frontiers in Microbiology, 2022
Ribonucleoside monophosphates are inevitably misincorporated into the DNA genome inside cells, and they need to be excised to avoid chromosome instability.
Jing Pang, Qinyu Guo, Zheng Lu
doaj   +1 more source

HIV-1 RT-Associated RNase H review

Archives of Pharmaceutical Sciences Ain Shams University, 2018
HIV-RT is an essential enzyme for HIV replication it comprises two associated functions: RNA- and DNA-dependent DNA polymerase (RDDP & DDDP) and ribonuclease H (RNase H).
Madonna M. A. Mitry, Nermin S. Abdou, Rabah A. T. Serya   +2 more
doaj   +1 more source

RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes

Bio-Protocol, 2015
The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA (Figure ...
Angela Corona, Enzo Tramontano
doaj   +1 more source

Membrane recognition and dynamics of the RNA Degradosome [PDF]

, 2015
RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Luisi, Ben, Syma Khalid (580907), Bouvier, Marie, Turlan, Catherine, Kebalo, J.M., Peyron, Pascale, Leonora Poljak, Peter J Bond, Peter J. Bond (688987), Strahl, H., Poljak, Leonora, Carpousis, A.J., Khalid, Syma, Ben F. Luisi (688991), Turlan, C., Henrik Strahl, Strahl, Henrik, Syma Khalid, Khalid, S., Jean-Marie Kebalo, Pascale Peyron, Henrik Strahl (688985), Catherine Turlan, Bouvier, M., Marie Bouvier, Agamemnon J. Carpousis (12453), Jean-Marie Kebalo (688988), Agamemnon J Carpousis, Pascale Peyron (239058), Leendert Hamoen (688990), Bond, Peter J., Bond, P.J., Ben F Luisi, Catherine Turlan (688986), Bond, Peter, Viollier, Patrick H., Marie Bouvier (260008), Hamoen, L.; id_orcid, Hamoen, Leendert, Luisi, Ben F., Carpousis, Agamemnon J., Peyron, P., Poljak, L., Luisi, B.F., Kebalo, Jean-Marie, Leendert Hamoen, Leonora Poljak (688989), Carpousis, Agamemnon, Hamoen, L.   +48 more
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Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes

BMC Evolutionary Biology, 2007
Background A theoretical model of genetic redundancy has proposed that the fates of redundant genes depend on the degree of functional redundancy, and that functionally redundant genes will not be inherited together.
Tomita Masaru, Kochiwa Hiromi, Kanai Akio   +2 more
doaj   +1 more source

The Basic Loop of the RNase H Domain of MLV RT Is Important Both for RNase H and for Polymerase Activity [PDF]

, 2001
Escherichia coli RNase H has a basic extension that is involved in binding nucleic acid substrates. This basic extension is present in the RNase H of Moloney murine leukemia virus reverse transcriptase (MLV RT), but has been deleted from the RNase H of ...
Gao, Hong-Qiang, Hughes, Stephen H., Boyer, Paul L., Frank, Peter, Clark, Patrick K.   +4 more
core   +1 more source

RNase H1 and H2 Are Differentially Regulated to Process RNA-DNA Hybrids

Cell Reports, 2019
Summary: RNA-DNA hybrids are tightly regulated to ensure genome integrity. The RNase H enzymes RNase H1 and H2 contribute to chromosomal stability through the removal of RNA-DNA hybrids.
Arianna Lockhart, Vanessa Borges Pires, Fabio Bento, Vanessa Kellner, Sarah Luke-Glaser, George Yakoub, Helle D. Ulrich, Brian Luke   +7 more
doaj   +1 more source