Results 21 to 30 of about 303,152 (296)

The Role of Conserved Residues in the DEDDh Motif: the Proton-Transfer Mechanism of HIV-1 RNase H [PDF]

, 2021
RNase H is a prototypical example for two-metal-ion catalysis in enzymes. An RNase H activity cleaving the ribonucleic acid (RNA) backbone of a DNA/RNA hybrid is present not only in important drug targets, such as the HIV-1 reverse transcriptase, but ...
Duerr, Simon L., Shao, Y, Denes Berta, Peter, C, Jambrina, PG, Rosta, Edina, Berta, D, Rosta, E, Dürr, SL, Dürr, Simon, Yihan Shao, Peter, Christine, García Jambrina, Pablo, Suardiaz, R, Reynier Suardiaz, Dürr, Simon L., Suardiaz, Reynier, Suardíaz Delrío, Reynier, Jambrina, Pablo, Dürr, Simon Leonard, Berta, Denes, Bohuszewicz, Olga, Bohuszewicz, O, Pablo Garcia Jambrina, Jambrina, Pablo G., Christine Peter, Olga Bohuszewicz, Edina Rosta, Simon L. Dürr, Shao, Yihan   +29 more
core   +1 more source

Acetylation regulates the oligomerization state and activity of RNase J, the Helicobacter pylori major ribonuclease

Nature Communications, 2023
In the gastric pathogen Helicobacter pylori, post-transcriptional regulation relies strongly on the activity of the essential ribonuclease RNase J. Here, we elucidated the crystal and cryo-EM structures of RNase J and determined that it assembles into ...
Alejandro Tejada-Arranz, Aleksei Lulla, Maxime Bouilloux-Lafont, Evelyne Turlin, Xue-Yuan Pei, Thibaut Douché, Mariette Matondo, Allison H. Williams, Bertrand Raynal, Ben F. Luisi, Hilde De Reuse   +10 more
doaj   +1 more source

The catalytic mechanism, metal dependence, substrate specificity, and biodiversity of ribonuclease H

Frontiers in Microbiology, 2022
Ribonucleoside monophosphates are inevitably misincorporated into the DNA genome inside cells, and they need to be excised to avoid chromosome instability.
Jing Pang, Qinyu Guo, Zheng Lu
doaj   +1 more source

2-(Arylamino)-6-(trifluoromethyl)nicotinic Acid Derivatives: New HIV-1 RT Dual Inhibitors Active on Viral Replication

Molecules, 2020
The persistence of the AIDS epidemic, and the life-long treatment required, indicate the constant need of novel HIV-1 inhibitors. In this scenario the HIV-1 Reverse Transcriptase (RT)-associated ribonuclease H (RNase H) function is a promising drug ...
Angela Corona, Valentina Onnis, Claudia Del Vecchio, Francesca Esposito, Yung-Chi Cheng, Enzo Tramontano   +5 more
doaj   +1 more source

HIV-1 RT-Associated RNase H review

Archives of Pharmaceutical Sciences Ain Shams University, 2018
HIV-RT is an essential enzyme for HIV replication it comprises two associated functions: RNA- and DNA-dependent DNA polymerase (RDDP & DDDP) and ribonuclease H (RNase H).
Madonna M. A. Mitry, Nermin S. Abdou, Rabah A. T. Serya   +2 more
doaj   +1 more source

Membrane recognition and dynamics of the RNA Degradosome [PDF]

, 2015
RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Luisi, Ben, Syma Khalid (580907), Bouvier, Marie, Turlan, Catherine, Kebalo, J.M., Peyron, Pascale, Leonora Poljak, Peter J Bond, Peter J. Bond (688987), Strahl, H., Poljak, Leonora, Carpousis, A.J., Khalid, Syma, Ben F. Luisi (688991), Turlan, C., Henrik Strahl, Strahl, Henrik, Syma Khalid, Khalid, S., Jean-Marie Kebalo, Pascale Peyron, Henrik Strahl (688985), Catherine Turlan, Bouvier, M., Marie Bouvier, Agamemnon J. Carpousis (12453), Jean-Marie Kebalo (688988), Agamemnon J Carpousis, Pascale Peyron (239058), Leendert Hamoen (688990), Bond, Peter J., Bond, P.J., Ben F Luisi, Catherine Turlan (688986), Bond, Peter, Viollier, Patrick H., Marie Bouvier (260008), Hamoen, L.; id_orcid, Hamoen, Leendert, Luisi, Ben F., Carpousis, Agamemnon J., Peyron, P., Poljak, L., Luisi, B.F., Kebalo, Jean-Marie, Leendert Hamoen, Leonora Poljak (688989), Carpousis, Agamemnon, Hamoen, L.   +48 more
core   +1 more source

Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes

BMC Evolutionary Biology, 2007
Background A theoretical model of genetic redundancy has proposed that the fates of redundant genes depend on the degree of functional redundancy, and that functionally redundant genes will not be inherited together.
Tomita Masaru, Kochiwa Hiromi, Kanai Akio   +2 more
doaj   +1 more source

Redesignation of the RNase D activity associated with retroviral reverse transcriptase as RNase H [PDF]

Journal of Virology, 1994
In the presence of Mn2+, reverse transcriptase of both human immunodeficiency virus and murine leukemia virus hydrolyzes duplex RNA. However, designating this novel activity RNase D conflicts with Escherichia coli RNase D, which participates in tRNA processing.
Z, Hostomsky, S H, Hughes, S P, Goff, S F, Le Grice   +3 more
openaire   +2 more sources

Ribonuclease H/DNA Polymerase HIV-1 Reverse Transcriptase Dual Inhibitor: Mechanistic Studies on the Allosteric Mode of Action of Isatin-Based Compound RMNC6. [PDF]

PLoS ONE, 2016
The DNA polymerase and ribonuclease H (RNase H) activities of human immunodeficiency virus type 1 (HIV-1) are needed for the replication of the viral genome and are validated drug targets.
Angela Corona, Rita Meleddu, Francesca Esposito, Simona Distinto, Giulia Bianco, Takashi Masaoka, Elias Maccioni, Luis Menéndez-Arias, Stefano Alcaro, Stuart F J Le Grice, Enzo Tramontano   +10 more
doaj   +1 more source

Polypurine Tract Formation by Ty1 RNase H [PDF]

Journal of Biological Chemistry, 2001
To better understand the mechanism by which Ty1 RNase H creates the polypurine tract (PPT) primer, we have demonstrated the polymerase-dependent hydrolytic activity of Ty1 reverse transcriptase (RT) during minus-strand synthesis. Using RNase H and polymerase mutants of the recombinant Ty1 RT protein, we show that the two domains of Ty1 RT can act ...
M, Wilhelm, O, Uzun, E H, Mules, A, Gabriel, F X, Wilhelm   +4 more
openaire   +2 more sources