Results 91 to 100 of about 14,336,830 (357)

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding

Retrovirology, 2012
Background The ribonuclease H (RNase H) domains of retroviral reverse transcriptases play an essential role in the replication cycle of retroviruses. During reverse transcription of the viral genomic RNA, an RNA/DNA hybrid is created whose RNA strand ...
Leo Berit, Schweimer Kristian, Rösch Paul, Hartl Maximilian J, Wöhrl Birgitta M   +4 more
doaj   +1 more source

The Sequential Mechanism of HIV Reverse Transcriptase RNase H [PDF]

Journal of Biological Chemistry, 2000
Synthesis of the minus strand of viral DNA by human immunodeficiency virus, type 1 (HIV-1) reverse transcriptase is accompanied by RNase H degradation of the viral RNA genome. RNA fragments remain after synthesis and are degraded by the polymerase-independent mode of RNase H cleavage.
Robert A. Bambara, Philip J. Fay, Mini Balakrishnan, Chockalingam Palaniappan, Michele Wisniewski   +4 more
openaire   +3 more sources

The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐xL or Mcl‐1 in ovarian cancer

Molecular Oncology, EarlyView.
The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, Marie Villedieu   +10 more
wiley   +1 more source

Rational engineering of DNA-nanoparticle motor with high speed and processivity comparable to motor proteins

Nature Communications
DNA-nanoparticle motor is a burnt-bridge Brownian ratchet moving on RNA-modified surface driven by Ribonuclease H (RNase H), and one of the fastest nanoscale artificial motors. However, its speed is still much lower than those of motor proteins.
Takanori Harashima, Akihiro Otomo, Ryota Iino   +2 more
doaj   +1 more source

N-Hydroxypiridinedione: A Privileged Heterocycle for Targeting the HBV RNase H

Molecules
Hepatitis B virus (HBV) remains a global health threat. Ribonuclease H (RNase H), part of the virus polymerase protein, cleaves the pgRNA template during viral genome replication.
Dimitrios Moianos, Maria Makri, Georgia-Myrto Prifti, Aristeidis Chiotellis, Alexandros Pappas, Molly E. Woodson, Razia Tajwar, John E. Tavis, Grigoris Zoidis   +8 more
doaj   +1 more source

Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains

Virology Journal, 2009
Reverse transcriptases from HIV-1 and MuLV respectively prefer Mg2+ and Mn2+ for their polymerase activity, with variable fidelity, on both RNA and DNA templates.
Pandey Virendra N, Upadhyay Alok, Talele Tanaji T   +2 more
doaj   +1 more source

One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts

Molecules, 2020
There is an increasing demand for efficient and robust production of short RNA molecules in both pharmaceutics and research. A standard method is in vitro transcription by T7 RNA polymerase.
Hannes Feyrer, Raluca Munteanu, Lorenzo Baronti, Katja Petzold   +3 more
doaj   +1 more source

Autonomously folding protein fragments reveal differences in the energy landscapes of homologous RNases H. [PDF]

PLoS ONE, 2015
An important approach to understanding how a protein sequence encodes its energy landscape is to compare proteins with different sequences that fold to the same general native structure. In this work, we compare E. coli and T.
Laura E Rosen, Susan Marqusee
doaj   +1 more source

Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells [PDF]

, 1978
The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml ...
Amalric, François, Attardi, Giuseppe, Crews, Stephen, Grohmann, Karel   +3 more
core   +1 more source