Results 11 to 20 of about 476,060 (306)
Cycling Probe Technology with RNase H Attached to an Oligonucleotide [PDF]
BioTechniques, 1996 A streptavidin-RNase H gene fusion was constructed by cloning the Thermus thermophilus RNase H coding sequence in the streptavidin expression vector pTSA18F.
F. Bekkaoui +4 more
doaj +3 more sources
Analysis and Molecular Determinants of HIV RNase H Cleavage Specificity at the PPT/U3 Junction [PDF]
Viruses, 2021 HIV reverse transcriptases (RTs) convert viral genomic RNA into double-stranded DNA. During reverse transcription, polypurine tracts (PPTs) resilient to RNase H cleavage are used as primers for plus-strand DNA synthesis.
Mar Álvarez +4 more
doaj +2 more sources
The “Connection” Between HIV Drug Resistance and RNase H [PDF]
Viruses, 2010 Currently, nucleoside reverse transcriptase inhibitors (NRTIs) and nonnucleoside reverse transcriptase inhibitors (NNRTIs) are two classes of antiretroviral agents that are approved for treatment of HIV-1 infection. Since both NRTIs and NNRTIs target the
Krista A. Delviks-Frankenberry +2 more
doaj +4 more sources
RNase H-defective mutants of Escherichia coli [PDF]
Journal of Bacteriology, 1986 Tokio Kogoma
openalex +4 more sources
RNase H As Gene Modifier, Driver of Evolution and Antiviral Defense [PDF]
Frontiers in Microbiology, 2017 Retroviral infections are ‘mini-symbiotic’ events supplying recipient cells with sequences for viral replication, including the reverse transcriptase (RT) and ribonuclease H (RNase H).
Karin Moelling +4 more
doaj +2 more sources
Nature Communications, 2023 In the gastric pathogen Helicobacter pylori, post-transcriptional regulation relies strongly on the activity of the essential ribonuclease RNase J. Here, we elucidated the crystal and cryo-EM structures of RNase J and determined that it assembles into ...
Alejandro Tejada-Arranz +10 more
doaj +1 more source
The catalytic mechanism, metal dependence, substrate specificity, and biodiversity of ribonuclease H
Frontiers in Microbiology, 2022 Ribonucleoside monophosphates are inevitably misincorporated into the DNA genome inside cells, and they need to be excised to avoid chromosome instability.
Jing Pang, Qinyu Guo, Zheng Lu
doaj +1 more source
HIV-1 RT-Associated RNase H review
Archives of Pharmaceutical Sciences Ain Shams University, 2018 HIV-RT is an essential enzyme for HIV replication it comprises two associated functions: RNA- and DNA-dependent DNA polymerase (RDDP & DDDP) and ribonuclease H (RNase H).
Madonna M. A. Mitry +2 more
doaj +1 more source
Molecules, 2020 The persistence of the AIDS epidemic, and the life-long treatment required, indicate the constant need of novel HIV-1 inhibitors. In this scenario the HIV-1 Reverse Transcriptase (RT)-associated ribonuclease H (RNase H) function is a promising drug ...
Angela Corona +5 more
doaj +1 more source
Insights into the structure and activity of prototype foamy virus RNase H
Retrovirology, 2012 Background RNase H is an endonuclease that hydrolyzes the RNA strand in RNA/DNA hybrids. Retroviral reverse transcriptases harbor a C-terminal RNase H domain whose activity is essential for viral replication.
Leo Berit +4 more
doaj +1 more source