RNase H and Its Effects on PCR [PDF]
BioTechniques, 2002 Swamy K. Polumuri +2 more
doaj +5 more sources
Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes [PDF]
BMC Evolutionary Biology, 2007 Background A theoretical model of genetic redundancy has proposed that the fates of redundant genes depend on the degree of functional redundancy, and that functionally redundant genes will not be inherited together.
Tomita Masaru +2 more
doaj +5 more sources
RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes [PDF]
Bio-Protocol, 2015 The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA (Figure ...
Angela Corona, Enzo Tramontano
doaj +2 more sources
Flavonoids and Acid-Hydrolysis derivatives of Neo-Clerodane diterpenes from Teucrium flavum subsp. glaucum as inhibitors of the HIV-1 reverse transcriptase–associated RNase H function [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2021 Bioassay-guided fractionation of the ethyl acetate extract from Teucrium flavum subsp. glaucum, endowed with inhibitory activity towards the HIV-1 reverse transcriptase–associated RNase H function, led to the isolation of salvigenin (1), cirsimaritin (2)
Benedetta Fois +8 more
doaj +2 more sources
Targeting HIV-1 RNase H: N’-(2-Hydroxy-benzylidene)-3,4,5-Trihydroxybenzoylhydrazone as Selective Inhibitor Active against NNRTIs-Resistant Variants [PDF]
Viruses, 2020 HIV-1 infection requires life-long treatment and with 2.1 million new infections/year, faces the challenge of an increased rate of transmitted drug-resistant mutations.
Angela Corona +12 more
doaj +2 more sources
RNase H activities counteract a toxic effect of Polymerase η in cells replicating with depleted dNTP pools. [PDF]
Nucleic Acids Res, 2019 RNA:DNA hybrids are transient physiological intermediates that arise during several cellular processes such as DNA replication. In pathological situations, they may stably accumulate and pose a threat to genome integrity.
Meroni A +8 more
europepmc +2 more sources
Identifying and avoiding off-target effects of RNase H-dependent antisense oligonucleotides in mice. [PDF]
Nucleic Acids Res, 2018 Antisense oligonucleotides that are dependent on RNase H for cleavage and subsequent degradation of complementary RNA are being developed as therapeutics. Besides the intended RNA target, such oligonucleotides may also cause degradation of unintended RNA
Hagedorn PH +11 more
europepmc +2 more sources
An RNase H‐Like gene complements resistance to Bean common mosaic necrosis virus in Phaseolus vulgaris [PDF]
The Plant GenomeBean common mosaic virus and Bean common mosaic necrosis virus (BCMNV) are related positive‐sense RNA potyviruses that limit the production of common bean (Phaseolus vulgaris L.) worldwide.
Alvaro Soler‐Garzón +1 more
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RNase H sequence preferences influence antisense oligonucleotide efficiency. [PDF]
Nucleic Acids Res, 2017 RNase H cleaves RNA in RNA–DNA duplexes. It is present in all domains of life as well as in multiple viruses and is essential for mammalian development and for human immunodeficiency virus replication.
Kielpinski LJ +3 more
europepmc +2 more sources
Analysis and Molecular Determinants of HIV RNase H Cleavage Specificity at the PPT/U3 Junction [PDF]
Viruses, 2021 HIV reverse transcriptases (RTs) convert viral genomic RNA into double-stranded DNA. During reverse transcription, polypurine tracts (PPTs) resilient to RNase H cleavage are used as primers for plus-strand DNA synthesis.
Mar Álvarez +4 more
doaj +2 more sources