RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection. [PDF]
Sci Rep, 2018 RNA-primed rolling circle amplification (RPRCA) is a useful laboratory method for RNA detection; however, the detection of RNA is limited by the lack of information on 3′-terminal sequences.
Takahashi H +7 more
europepmc +2 more sources
The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. [PDF]
PLoS Genetics, 2017 RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity.
Juan Lafuente-Barquero +8 more
doaj +4 more sources
Developing and Evaluating Inhibitors against the RNase H Active Site of HIV-1 Reverse Transcriptase. [PDF]
J Virol, 2018 We tested three compounds for their ability to inhibit the RNase H (RH) and polymerase activities of HIV-1 reverse transcriptase (RT). A high-resolution crystal structure (2.2 Å) of one of the compounds showed that it chelates the two magnesium ions at ...
Boyer PL +7 more
europepmc +2 more sources
RNase H sequence preferences influence antisense oligonucleotide efficiency. [PDF]
Nucleic Acids Res, 2019 RNase H cleaves RNA in RNA-DNA duplexes. It is present in all domains of life as well as in multiple viruses and is essential for mammalian development and for human immunodeficiency virus replication.
Kiełpiński ŁJ +3 more
europepmc +2 more sources
PLoS Genetics, 2014 During transcription, the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout the budding yeast genome.
Aziz El Hage +3 more
doaj +2 more sources
Cycling Probe Technology with RNase H Attached to an Oligonucleotide [PDF]
BioTechniques, 1996 A streptavidin-RNase H gene fusion was constructed by cloning the Thermus thermophilus RNase H coding sequence in the streptavidin expression vector pTSA18F.
F. Bekkaoui +4 more
doaj +3 more sources
Identification of HIV-1 Reverse Transcriptase-Associated Ribonuclease H Inhibitors Based on 2-Hydroxy-1,4-naphthoquinone Mannich Bases [PDF]
MoleculesThere is a strong demand for new and efficient antiviral compounds. A series of 2-hydroxy-1,4-naphthoquinone Mannich bases were screened for their HIV-1-RNase H inhibitory activity.
Nhat Quang Tu +9 more
doaj +3 more sources
The “Connection” Between HIV Drug Resistance and RNase H [PDF]
Viruses, 2010 Currently, nucleoside reverse transcriptase inhibitors (NRTIs) and nonnucleoside reverse transcriptase inhibitors (NNRTIs) are two classes of antiretroviral agents that are approved for treatment of HIV-1 infection. Since both NRTIs and NNRTIs target the
Krista A. Delviks-Frankenberry +2 more
doaj +4 more sources
RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair. [PDF]
EMBO Rep, 2018 Zhao H, Zhu M, Limbo O, Russell P.
europepmc +2 more sources
Crystal structure of archaeal RNase HII: a homologue of human major RNase H [PDF]
Structure, 2000 RNases H are present in all organisms and cleave RNAs in RNA/DNA hybrids. There are two major types of RNases H that have little similarity in sequence, size and specificity. The structure of RNase HI, the smaller enzyme and most abundant in bacteria, has been extensively studied.
Luhua Lai +4 more
openalex +5 more sources