Results 21 to 30 of about 476,060 (306)

Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes

BMC Evolutionary Biology, 2007
Background A theoretical model of genetic redundancy has proposed that the fates of redundant genes depend on the degree of functional redundancy, and that functionally redundant genes will not be inherited together.
Tomita Masaru, Kochiwa Hiromi, Kanai Akio   +2 more
doaj   +1 more source

Ribonuclease H/DNA Polymerase HIV-1 Reverse Transcriptase Dual Inhibitor: Mechanistic Studies on the Allosteric Mode of Action of Isatin-Based Compound RMNC6. [PDF]

PLoS ONE, 2016
The DNA polymerase and ribonuclease H (RNase H) activities of human immunodeficiency virus type 1 (HIV-1) are needed for the replication of the viral genome and are validated drug targets.
Angela Corona, Rita Meleddu, Francesca Esposito, Simona Distinto, Giulia Bianco, Takashi Masaoka, Elias Maccioni, Luis Menéndez-Arias, Stefano Alcaro, Stuart F J Le Grice, Enzo Tramontano   +10 more
doaj   +1 more source

The Unfolding Behavior of RNase H Under Force [PDF]

Biophysical Journal, 2010
We have used optical tweezers to revisit the energy landscape of E. coli RNase H under mechanical force. This protein's equilibrium energetics and folding pathway have been studied in bulk and in single-molecule mechanical denaturation experiments, which showed the presence of a collapsed folding intermediate that is on-pathway to the native state (1 ...
Susan Marqusee, Jesse Dill, Carlos Bustamante   +2 more
openaire   +2 more sources

Bistability of an In Vitro Synthetic Autoregulatory Switch [PDF]

ACS Synthetic Biology 2012, 2011
The construction of synthetic biochemical circuits is an essential step for developing quantitative understanding of information processing in natural organisms. Here, we report construction and analysis of an in vitro circuit with positive autoregulation that consists of just four synthetic DNA strands and three enzymes, bacteriophage T7 RNA ...
arxiv   +1 more source

Single-domain protein folding: a multi-faceted problem [PDF]

FROM PHYSICS TO BIOLOGY: The Interface between Experiment and Computation - BIFI 2006 II International Congress. AIP Conference Proceedings, Volume 851, pp. 70-95 (2006), 2008
We review theoretical approaches, experiments and numerical simulations that have been recently proposed to investigate the folding problem in single-domain proteins. From a theoretical point of view, we emphasize the energy landscape approach. As far as experiments are concerned, we focus on the recent development of single-molecule techniques.
arxiv   +1 more source

Redesignation of the RNase D activity associated with retroviral reverse transcriptase as RNase H [PDF]

Journal of Virology, 1994
In the presence of Mn2+, reverse transcriptase of both human immunodeficiency virus and murine leukemia virus hydrolyzes duplex RNA. However, designating this novel activity RNase D conflicts with Escherichia coli RNase D, which participates in tRNA processing.
Z. Hostomsky, S. P. Goff, S.H. Hughes, S. F. J. Le Grice   +3 more
openaire   +3 more sources

The Basic Loop of the RNase H Domain of MLV RT Is Important Both for RNase H and for Polymerase Activity

Virology, 2001
Escherichia coli RNase H has a basic extension that is involved in binding nucleic acid substrates. This basic extension is present in the RNase H of Moloney murine leukemia virus reverse transcriptase (MLV RT), but has been deleted from the RNase H of HIV-1 RT. Previous work showed that removing the basic loop from MLV RT (the mutant is called DeltaC)
Stephen H. Hughes, Paul L. Boyer, Hong-Qiang Gao, Patrick K. Clark, Peter Frank   +4 more
openaire   +3 more sources

Selective inhibition of RNase H by dextran.

Journal of Biological Chemistry, 1981
Ordinarily, ribonuclease H hydrolyzes poly(rA) . poly(dT) and phiX174DNA-RNA at equal rates. Here we show that in the presence of dextran, the degradation of poly(rA) . poly(dT) is inhibited, while that of phi 174DNA-RNA is not. A similar inhibition by sucrose is found to be due to trace contamination of dextran in the sucrose. Ribose, deoxyribose, and
M L Dirksen, R J Crouch
openaire   +3 more sources

Polypurine Tract Formation by Ty1 RNase H [PDF]

Journal of Biological Chemistry, 2001
To better understand the mechanism by which Ty1 RNase H creates the polypurine tract (PPT) primer, we have demonstrated the polymerase-dependent hydrolytic activity of Ty1 reverse transcriptase (RT) during minus-strand synthesis. Using RNase H and polymerase mutants of the recombinant Ty1 RT protein, we show that the two domains of Ty1 RT can act ...
Ozcan Uzun, F.X. Wilhelm, Emilie H. Mules, M.L. Wilhelm, Abram Gabriel   +4 more
openaire   +3 more sources

Scaffold hopping and optimisation of 3’,4’-dihydroxyphenyl- containing thienopyrimidinones: synthesis of quinazolinone derivatives as novel allosteric inhibitors of HIV-1 reverse transcriptase-associated ribonuclease H

Journal of Enzyme Inhibition and Medicinal Chemistry, 2020
Bioisosteric replacement and scaffold hopping are powerful strategies in drug design useful for rationally modifying a hit compound towards novel lead therapeutic agents.
Graziella Tocco, Francesca Esposito, Pierluigi Caboni, Antonio Laus, John A. Beutler, Jennifer A. Wilson, Angela Corona, Stuart F. J. Le Grice, Enzo Tramontano   +8 more
doaj   +1 more source