Results 51 to 60 of about 476,060 (306)

The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding

Retrovirology, 2012
Background The ribonuclease H (RNase H) domains of retroviral reverse transcriptases play an essential role in the replication cycle of retroviruses. During reverse transcription of the viral genomic RNA, an RNA/DNA hybrid is created whose RNA strand ...
Leo Berit, Schweimer Kristian, Rösch Paul, Hartl Maximilian J, Wöhrl Birgitta M   +4 more
doaj   +1 more source

Cell‐free and extracellular vesicle microRNAs with clinical utility for solid tumors

Molecular Oncology, EarlyView.
Cell‐free microRNAs (cfmiRs) are small‐RNA circulating molecules detectable in almost all body biofluids. Innovative technologies have improved the application of cfmiRs to oncology, with a focus on clinical needs for different solid tumors, but with emphasis on diagnosis, prognosis, cancer recurrence, as well as treatment monitoring.
Yoshinori Hayashi, Janelle‐Cheri Millen, Romela Irene Ramos, Jennifer A. Linehan, Timothy G. Wilson, Dave S. B. Hoon, Matias A. Bustos   +6 more
wiley   +1 more source

Rational engineering of DNA-nanoparticle motor with high speed and processivity comparable to motor proteins

Nature Communications
DNA-nanoparticle motor is a burnt-bridge Brownian ratchet moving on RNA-modified surface driven by Ribonuclease H (RNase H), and one of the fastest nanoscale artificial motors. However, its speed is still much lower than those of motor proteins.
Takanori Harashima, Akihiro Otomo, Ryota Iino   +2 more
doaj   +1 more source

N-Hydroxypiridinedione: A Privileged Heterocycle for Targeting the HBV RNase H

Molecules
Hepatitis B virus (HBV) remains a global health threat. Ribonuclease H (RNase H), part of the virus polymerase protein, cleaves the pgRNA template during viral genome replication.
Dimitrios Moianos, Maria Makri, Georgia-Myrto Prifti, Aristeidis Chiotellis, Alexandros Pappas, Molly E. Woodson, Razia Tajwar, John E. Tavis, Grigoris Zoidis   +8 more
doaj   +1 more source

Autonomously folding protein fragments reveal differences in the energy landscapes of homologous RNases H. [PDF]

PLoS ONE, 2015
An important approach to understanding how a protein sequence encodes its energy landscape is to compare proteins with different sequences that fold to the same general native structure. In this work, we compare E. coli and T.
Laura E Rosen, Susan Marqusee
doaj   +1 more source

Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM

Molecular Oncology, EarlyView.
CircRNAs are differentially expressed in glioblastoma primary tumors and might serve as therapeutic targets and diagnostic markers. The investigation of circRNA and RNA‐binding proteins (RBPs) interactions shows that distinct RBPs play a role in circRNA biogenesis and function.
Julia Latowska‐Łysiak, Żaneta Zarębska, Marcin P. Sajek, Adriana Grabowska, Alessia Buratin, Paweł Głodowicz, Julia O. Misiorek, Konrad Kuczyński, Stefania Bortoluzzi, Marek Żywicki, Jan G. Kosiński, Agnieszka Rybak‐Wolf, Rafał Piestrzeniewicz, Anna M. Barciszewska, Katarzyna Rolle   +14 more
wiley   +1 more source

On the Second Kahn--Kalai Conjecture [PDF]

arXiv, 2022
For any given graph $H$, we are interested in $p_\mathrm{crit}(H)$, the minimal $p$ such that the Erd\H{o}s-R\'enyi graph $G(n,p)$ contains a copy of $H$ with probability at least $1/2$. Kahn and Kalai (2007) conjectured that $p_\mathrm{crit}(H)$ is given up to a logarithmic factor by a simpler "subgraph expectation threshold" $p_\mathrm{E}(H)$, which ...
arxiv  

Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains

Virology Journal, 2009
Reverse transcriptases from HIV-1 and MuLV respectively prefer Mg2+ and Mn2+ for their polymerase activity, with variable fidelity, on both RNA and DNA templates.
Pandey Virendra N, Upadhyay Alok, Talele Tanaji T   +2 more
doaj   +1 more source

1,2,4-Triazolo[1,5-a]pyrimidines as a Novel Class of Inhibitors of the HIV-1 Reverse Transcriptase-Associated Ribonuclease H Activity

Molecules, 2020
Despite great efforts have been made in the prevention and therapy of human immunodeficiency virus (HIV-1) infection, however the difficulty to eradicate latent viral reservoirs together with the emergence of multi-drug-resistant strains require the ...
Jenny Desantis, Serena Massari, Angela Corona, Andrea Astolfi, Stefano Sabatini, Giuseppe Manfroni, Deborah Palazzotti, Violetta Cecchetti, Christophe Pannecouque, Enzo Tramontano, Oriana Tabarrini   +10 more
doaj   +1 more source

Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. [PDF]

PLoS ONE, 2014
Antisense oligonucleotides (ASOs) are most commonly designed to reduce targeted RNA via RNase H1-dependent degradation. In this paper we demonstrate that cellular proteins can compete for sites targeted by RNase H1-dependent ASOs.
Timothy A Vickers, Stanley T Crooke
doaj   +1 more source