Results 181 to 190 of about 455,663 (298)

β‐Triketone‐Based Ionizable Cationic Lipids Synthesized via Click Chemistry for siRNA Delivery

Advanced Science, EarlyView.
This study innovatively applies β‐triketone‐based click chemistry in the synthesis of ionizable cationic lipids (ICLs), which allows the generation of a lipid library in large quantities and in a short time. The ICLs identified achieved effective lipid nanoparticles‐mediated hepatic gene silencing in vivo using siRNA, and computational simulations are ...
Huatian Li, Haocheng Tang, Yiqing Mu, Shangyu Chen, Paul Edward Floreancig, Junmei Wang, Yixian Huang, Song Li   +7 more
wiley   +1 more source

Synthetic riboswitches for the analysis of tRNA processing by eukaryotic RNase P enzymes. [PDF]

RNA, 2022
Ender A, Grafl N, Kolberg T, Findeiß S, Stadler PF, Mörl M.   +5 more
europepmc   +1 more source

DOT1L Drives Endothelial‐to‐Mesenchymal Transition and Fibrotic Vascular Remodeling via H3K79 Methylation

Advanced Science, EarlyView.
DOT1L as a central epigenetic regulator of EndoMT and pulmonary fibrosis. Acting as an early epigenetic switch, it translates TGFβ–SMAD signaling into H3K79me2‐mediated chromatin remodeling, selectively activates fibrosis‐related genes, and primes ECs for rapid mesenchymal transition.
Yaofeng Wang, Xing Peng, Jingjing Chen, Yun Zhang, Tinghong Zhang, Jingyuan Zhang, Jiaying Fan, Hui Zheng, Qiaoyuan Liu, Zhimin Song, Zhan‐Peng Huang, Shu Meng   +11 more
wiley   +1 more source

Coevolution of RNase P and the ribosome. [PDF]

Proc Natl Acad Sci U S A
Petrov AS, Alvarez-Carreño C, Dean Williams L, Ditzler MA.   +3 more
europepmc   +1 more source

An Artificial Antibody‐Based Toolbox Accelerates Validation of Hidden Microproteins Encoded by the Dark Genome

Advanced Science, EarlyView.
Microproteins are hidden treasures encoded by the “dark proteome” but remain largely underexplored due to the lack of highly efficient tools. We developed a molecularly imprinted polymers (MIPs)‐based toolbox (CLAIMID) to achieve accelerated and ultrasensitive microproteins validation at multiple biological scales (single living cells, cell populations,
Hui He, Anqi Zhang, Zhanchen Guo, Qi Li, Song Gao, Wenyan Guan, Ying Li, Jingran Chen, Sijia Sun, Jian He, Zhen Liu   +10 more
wiley   +1 more source

Resurrecting the Activity of the RNA Subunit of Human RNase P. [PDF]

Chembiochem
Li D, Kirsebom LA, Hartmann RK.
europepmc   +1 more source

Structural basis of RNA processing by human mitochondrial RNase P. [PDF]

Nat Struct Mol Biol, 2021
Bhatta A, Dienemann C, Cramer P, Hillen HS.   +3 more
europepmc   +1 more source

An Open‐Source Pipeline for Calcium Imaging and All‐Optical Physiology in Human Stem Cell‐Derived Neurons

Advanced Science, EarlyView.
This work introduces an open‐source all‐optical platform for functional phenotyping of human stem cell‐derived neurons. The system integrates optogenetics, calcium imaging, automated acquisition, and analysis to resolve single‐cell and network activity, enabling longitudinal measurements, disease modeling, and pharmacological screening in preclinical ...
Wardiya Afshar‐Saber, Federico M. Gasparoli, Ziqin Yang, Nicole A. Teaney, Rachel Hobson, Lahin Lalani, Gayathri Srinivasan, Dosh Whye, Ranit Karmakar, Elizabeth D. Buttermore, Kellen D. Winden, Cidi Chen, Mustafa Sahin   +12 more
wiley   +1 more source

Substrate and enzyme determinants for recognition by human mitochondrial RNase P. [PDF]

Nucleic Acids Res
Hazisllari E, Radovanović D, Toth U, Vilardo E, Hartmann RK, Rossmanith W.   +5 more
europepmc   +1 more source

Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme. [PDF]

Methods Enzymol, 2021
Zahurancik WJ, Norris AS, Lai SM, Snyder DT, Wysocki VH, Gopalan V.   +5 more
europepmc   +1 more source