An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum
Advanced Science, EarlyView.This work presents a strategy for engineering thermophilic Ago proteins to obtain wide catalytic temperature range and broad substrate spectrum. This strategy is applied to engineer PfAgo and TtdAgo, and the biochemical properties of the engineered PfAgo are systematically characterized. The study further elucidates the structural differences involved.
Longyu Wang +11 more
wiley +1 more source
In vitro analysis of processing at the 3'-end of precursors of M1 RNA, the catalytic subunit of Escherichia coli RNase P: Multiple pathways and steps for the processing [PDF]
, 1999 Semi Kim, Soyeong Sim, Younghoon Lee
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Collaborative Duality of CircGLIS3(2) RNA and Protein in human Wound Repair
Advanced Science, EarlyView.CircGLIS3(2), a circRNA transiently upregulated in dermal fibroblasts during acute wound healing, is induced by IL‐1α and TGF‐β. Its RNA activates fibroblasts into matrix‐secreting cells, while its encoded protein enhances proliferation. The RNA stabilizes PCOLCE, and the protein interacts with BTF3, collectively accelerating wound repair. Abstract The
Guanglin Niu +20 more
wiley +1 more source
Maturation of Pre-tRNAfMet by Escherichia coli RNase P Is Specified by a Guanosine of the 5′-Flanking Sequence [PDF]
, 1995 Thierry Meinnel, Sylvain Blanquet
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The Dynamic Network of RNP RNase P Subunits. [PDF]
Int J Mol Sci, 2021 Shaukat AN +3 more
europepmc +1 more source
Recent Advances in mRNA Delivery Systems for Cancer Therapy
Advanced Science, EarlyView.This review systematically investigates the applications of mRNA therapy in cancer treatment, with particular emphasis on nonviral delivery systems, targeting strategies, stimulus‐responsive systems, and local delivery methods. Concluding with a meticulous evaluation, the review sheds light on the prevailing challenges while illuminating promising ...
Zheng Zhang +9 more
wiley +1 more source
Structure-function analysis in nuclear RNase P RNA [PDF]
, 1996 David R. Engelke +2 more
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Synthetic riboswitches for the analysis of tRNA processing by eukaryotic RNase P enzymes. [PDF]
RNA, 2022 Ender A +5 more
europepmc +1 more source
Advanced Science, EarlyView.This study has identified lncRNA‐MEG3 as a key regulator of muscle mass, promoting slow‐twitch muscle fibers and preventing muscle atrophy. By stabilizing the SUZ12/PRC2 complex through liquid–liquid phase separation (LLPS), lncRNA‐MEG3 influences mitochondrial function and lipid metabolism.
Yilong Yao +15 more
wiley +1 more source
Solution structure and metal-ion binding of the P4 element from bacterial RNase P RNA [PDF]
, 2000 M. Lienhard Schmitz, Ignacio Tinoco
openalex +1 more source