Results 61 to 70 of about 436,820 (281)

Decrease in gyrase A protein expression in _E. coli_ cells inhibited by antisense ribozymes [PDF]

, 2008
RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. Nucleic acid-based gene interference technologies represent promising strategies for specific inhibition of
Malathi Raghunathan, Shilpakala Sainath Rao   +1 more
core   +1 more source

Timing molecular motion and production with a synthetic transcriptional clock [PDF]

, 2011
The realization of artificial biochemical reaction networks with unique functionality is one of the main challenges for the development of synthetic biology. Due to the reduced number of components, biochemical circuits constructed in vitro promise to be
Atkinson, Babendure, Buchler, Chamberlin, Danino, Del Vecchio, Dittmer, E. Franco, E. Friedrichs, E. Winfree, Elowitz, F. C. Simmel, Faeder, Ferrari, Fung, Grate, Green, Ishikawa, J. Kim, Kim, Kim, Kurin-Cs  rgei, Liedl, Lima, Luisi, McCarrey, Muller, Panyutin, R. Jungmann, R. Murray, Simpson, Stricker, Tigges, Yurke, Zuker   +34 more
core   +3 more sources

Cytomegalovirus infection is common in prostate cancer and antiviral therapies inhibit progression in disease models

Molecular Oncology, EarlyView.
Human cytomegalovirus infection is common in normal prostate epithelium, prostate tumor tissue, and prostate cancer cell lines. CMV promotes cell survival, proliferation, and androgen receptor signaling. Anti‐CMV pharmaceutical compounds in clinical use inhibited cell expansion in prostate cancer models in vitro and in vivo, motivating investigation ...
Johanna Classon, Moa Stenudd, Margherita Zamboni, Kanar Alkass, Carl‐Johan Eriksson, Lars Pedersen, Alrik Schörling, Anna Thoss, Anders Bergh, Pernilla Wikström, Hans‐Olov Adami, Henrik Toft Sørensen, Henrik Druid, Jonas Frisén   +13 more
wiley   +1 more source

Engineered RNase P Ribozymes Effectively Inhibit Human Cytomegalovirus Gene Expression and Replication

Viruses, 2014
RNase P ribozyme can be engineered to be a sequence-specific gene-targeting agent with promising application in both basic research and clinical settings.
Zhu Yang, Gia-Phong Vu, Hua Qian, Yuan-Chuan Chen, Yu Wang, Michael Reeves, Ke Zen, Fenyong Liu   +7 more
doaj   +1 more source

Evolutionary conundrum of RNase P: from a catalytic RNA form to a protein only enzyme form [PDF]

, 2019
RNaza P je esencijalna endonukleaza koja katalizira procesiranje 5'-vodećeg slijeda pre-tRNA. Postoje dva tipa RNaze P: RNA-ovisni enzim tj ribonukleoprotein te isključivo proteinska RNaza P (PRORP).
Radman, Katarina
core  

The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 angstrom resolution [PDF]

, 2008
Maturation of tRNA precursors into functional tRNA molecules requires trimming of the primary transcript at both the 5' and 3' ends. Cleavage of nucleotides from the 3' stem of tRNA precursors, releasing nucleotide diphosphates, is accomplished in ...
Blagova, Elena V., Fogg, Mark J., Levdikov, Vladimir M., Rawlings, Andrea E., Wilkinson, Anthony J., Wilson, Keith S.   +5 more
core   +2 more sources

Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts [PDF]

, 2020
Extracellular and intracellular degradation of nucleic acids remains an issue in non-viral gene therapy. Understanding biodegradation is critical for the rational design of gene therapeutics in order to maintain stability and functionality at the target ...
Braeckmans, Kevin, De Smedt, Stefaan, Raes, Laurens, Remaut, Katrien, Rombouts, Koen, Xiong, Ranhua, Zhang, Heyang   +6 more
core   +1 more source

Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Molecular Oncology, EarlyView.
Small extracellular vesicles are a promising source of diagnostic molecules. We conducted a comprehensive study, including transcriptome profiling and RT‐qPCR validation on large cohorts of samples. Diagnostic panels enabling sensitive detection of colorectal cancer and precancerous lesions were established. Some molecules were differentially expressed
Petra Vychytilova‐Faltejskova, Marketa Pavlikova, Lucie Pifkova, Robin Jugas, Tana Machackova, Lenka Radova, Milana Sachlova, Renata Bartosova, Tetiana Samoilenko, Zuzana Feckova, Jana Orlickova, Erika Slamova, Elleni Ponechal Michu, Dagmar Al Tukmachi, Michaela Ruckova, Martina Vodinska, Jan Kotoucek, Tina Catela Ivkovic, Marie Boudna, Lucia Bohovicova, Teodor Stanek, Jana Halamkova, Martin Svoboda, Vladimir Prochazka, Tomas Grolich, Zdenek Kala, Ondrej Slaby   +26 more
wiley   +1 more source

Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme

Nature Communications, 2019
Ribonulease P is a conserved ribozyme present in all kingdoms of life that is involved in the 5′ maturation step of tRNAs. Here the authors determine the structure of an archaeal RNase P holoenzyme that reveals how archaeal RNase P recognizes its tRNA ...
Futang Wan, Qianmin Wang, Jing Tan, Ming Tan, Juan Chen, Shaohua Shi, Pengfei Lan, Jian Wu, Ming Lei   +8 more
doaj   +1 more source

Synergies between RNA degradation and trans-translation in Streptococcus pneumoniae: cross regulation and co-transcription of RNase R and SmpB

BMC Microbiology, 2012
Background Ribonuclease R (RNase R) is an exoribonuclease that recognizes and degrades a wide range of RNA molecules. It is a stress-induced protein shown to be important for the establishment of virulence in several pathogenic bacteria. RNase R has also
Moreira Ricardo N, Domingues Susana, Viegas Sandra C, Amblar Mónica, Arraiano Cecília M   +4 more
doaj   +1 more source