Results 251 to 260 of about 97,073 (279)

Multivariate quality assessment of aged RP-HPLC columns

Journal of Chemometrics, 1996
The inner structure of RP-HPLC columns can change ('age') during routine use. The columns may be monitored to check the magnitude and type of the changes. One approach is to investigate, besides the chromatograms, the retention behaviour of a few selected solutes at different mobile phases (markers) during routine use. A distinction can be made between
Bolck, A., Smilde, A. K., Duineveld, K. A. A., Coenegracht, P. M. J.   +3 more
openaire   +3 more sources

Assay of CCNU in Tumor by RP-HPLC

Artificial Cells, Blood Substitutes, and Biotechnology, 2005
This paper reports a RP-HPLC method for the determination of Lomustine (CCNU) in mouse tumor tissue. CCNU was separated and determined on a reverse-phase C18 5 microm column with a mobile phase of H2O-acetonitrile (54:46), detected at 254 nm and no internal standard. The calibration curve was linear (r = 0.9999) within the range of 0.98-15.68 microg/ml
Zhao, Z. Q., Sun, Y., Zhang, Q. Q., Jiang, J.   +3 more
openaire   +2 more sources

Purification of Large “Troublesome” Polypeptides by RP-HPLC

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol addresses the group of large polypeptides that have proved to be troublesome to handle because of their low solubility or the presence of secondary structural elements that favor supramolecular self-self assembly. Included in this group are polypeptides with amphipathic α-helical or β-sheet structures with extensive runs of ...
Hooi Hong, Keah, Milton T W, Hearn
openaire   +2 more sources

Some new selective stationary phases for RP‐HPLC

Journal of Separation Science, 2010
AbstractAt the present time, more complex analyses of apolar compounds with similar chemical structures or of polar compounds, especially basic ones, having diverse properties require more selective stationary phases having better stabilities. This paper describes several new stationary phases with directed selectivities that were prepared by ...
Isabel C S F, Jardim, Liane, Maldaner, Josimara, Lourenço, Livia M A, Fioravanti, Carol H, Collins   +4 more
openaire   +2 more sources

Review On: RP-HPLC

Pharmaceutical analysis plays a vital role in determining the qualitative and quantitative composition of materials, essential for understanding sample characteristics. Analytical chemistry rifts into two branches qualitative, which classifies components, and quantitative, which measures them.
Akshata M. Girase, Bhupendra M. Mahale, Amitkumar R.Dhankani, Sunil p. pawar   +3 more
openaire   +1 more source

[RP-HPLC characteristics of dragon's blood].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2008
To study the fingerprint of dragon's blood resina draconis by high performance liquid chromatography.The samples are extracted with methanol and separated on a Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) with the mobile phase of acetonitrile-H2O in gradient mode, and the flow rate was 1.0 mL x min(-1), the detection wavelength was 275 nm and the
Xiu-Li, Gao, Qian, Jiang, Peng-Jiao, Wang, Min, Zhang   +3 more
openaire   +1 more source

Resolution Optimization in Ion-Interaction RP-HPLC.

Analytical Letters, 1997
Abstract Since in ion-interaction chromatography the dependence of the retention on the concentration of the ion-interaction reagent (IIR) follows different behaviours for the different analytes, the adjustment of IIR concentration can usefully be used to optimize resolution.
M. C. Gennaro, R. Aigotti, S. Angelino
openaire   +1 more source

A NOVEL PACKING MATERIAL FOR RP-HPLC

Journal of Liquid Chromatography & Related Technologies, 2000
A simple, practical, and economical procedure had been developed to prepare novel C18 ether-bond reversed phase packing. A soft and long C18 alkyl chain was coupled onto silica (Sinopak-S, particle size d = 5μm, pore diameter Dp=11nm, and surface area s=170m2/g) with γ-glycidoxypropyltrimethoxysilane as a coupling agent.
Yu Xin, Zhao Rui, Liu Guoquan
openaire   +1 more source

[RP-HPLC characteristics of Panax notoginseng].

Yao xue xue bao = Acta pharmaceutica Sinica, 2008
To develop the chemical characteristics of Panax notoginseng in order to control its quality.The samples was extracted using pressurized liquid extraction (PLE) and analyzed by HPLC-DAD. The data were evaluated using the "similarity evaluation system for chromatographic fingerprint of TCM" software (version 2004A).The chromatographic characteristics of
Jian-bo, Wan, Shao-ping, Li, Yi-tao, Wang   +2 more
openaire   +1 more source

Razdvajanje bjelančevina žitarica RP-HPLC metodom

2005
Za analizu bjelančevina žitarica, zbog njihove heterogenosti, slabe topljivosti i tendencije polimerizaciji razvijaju se i primjenjuju nove metode kojima se postiže bolja rezolucija i ponovljivost pri razdvajanju. Širu primjenu našle su elektroforetske i kromatografske metode.
Horvat, Daniela, Šimić, Gordana, Sudar, Rezica, Jurković, Zorica   +3 more
openaire   +2 more sources