Results 61 to 70 of about 97,740 (305)

Enzymatic DNA Reaction Networks for Orchestrating Stimuli‐Dependent Temporal Molecular Pulse

Advanced Science, EarlyView.
We present an enzymatic DNA reaction network (EDRN) that encodes nucleic‐acid targets in time, converting inputs into a universal strand and then into programmable transient fluorescence pulses. With time‐color multiplexing, EDRN enables single‐tube high‐plex nucleic acid detection and shows strong agreement with clinical sequencing across 32 specimens.
Jiayu Yang, Yali Chang, Zibin Chu, Linghao Zhang, Tengfang Zhang, Dai Erhei, Xin Su, Zhe Yin   +7 more
wiley   +1 more source

Rapid HPLC determination of ciprofloxacin, ofloxacin, and marbofloxacin alone or in a mixture

Results in Chemistry, 2023
A simple, sensitive, and accurate chromatography (RP-HPLC) method for simultaneous estimation of Ciprofloxacin, Ofloxacin, and Marbofloxacin in their combined pharmaceutical dosage form or individually. The HPLC separation was achieved on a Hypersil (C18,
Kh. Elgendy, M. Zaky, T. Alaa Eldin, S. Fadel   +3 more
doaj   +1 more source

Protein processing characterized by a gel-free proteomics approach [PDF]

, 2008
We describe a method for the specific isolation of representative N-terminal peptides of proteins and their proteolytic fragments. Their isolation is based on a gel-free, peptidecentric proteomics approach using the principle of diagonal chromatography ...
A. Staes, C. C. Wu, D. N. Perkins, J. Ji, J. Krijgsveld, K. Gevaert, K. Gevaert, K. Gevaert, K. Kuhn, L. Martens, L. McDonald, M. Mann, M. Miyagi, P. Damme Van, P. Meuleman, R. J. Beynon, S. E. Ong, Z. Szeltner   +17 more
core   +1 more source

Erasable FAP Targeted Spray‐On Probe for Fluorescence‐Guided Surgery

Advanced Science, EarlyView.
We present the first erasable spray‐on probe for fluorescence‐guided surgery. Following topical delivery, FAP‐FLASH550 targets fibroblast activation protein–expressing stromal cells, delineating tumor margins with high tumor‐to‐background ratios. A 365 nm UV light pulse rapidly erases fluorescent signals through triazene photodegradation and subsequent
Zachary Rabinowitz, Riley J. Deutsch‐Williams, Ralph Weissleder   +2 more
wiley   +1 more source

Development and validation of analytical method for the estimation of lamivudine in rabbit plasma

Journal of Pharmaceutical Analysis, 2011
Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma.
Akhilesh Vikram Singh, Lila K. Nath, Nihar R. Pani   +2 more
doaj   +1 more source

Methionine 129 variant of human prion protein oligomerizes more rapidly than the valine 129 variant - Implications for disease susceptibility to Creutzfeldt-Jakob disease [PDF]

, 2004
The human PrP gene (PRNP) has two common alleles that encode either methionine or valine at codon 129. This polymorphism modulates disease susceptibility and phenotype of human transmissible spongiform encyphalopathies, but the molecular mechanism by ...
Abdessamad Tahiri-Alaoui, Andrew C. Gill, Petra Disterer, William James, Prusiner, Weissmann, Will, Bruce, Hill, Prusiner, Prusiner, Collinge, Windl, Palmer, Brown, Baker, Cervenakova, Parchi, Hill, Collinge, Hauw, Goldfarb, Goldfarb, Dlouhy, Dermaut, Croes, Liemann, Riek, Alonso, Baskakov, Jackson, Baskakov, Gill, Lu, Lu, Landon, Safar, Bessen, Zahn, Riek, Nguyen, Tahiri-Alaoui, Santini, Wong, Wildegger, Matouschek, Vendruscolo, Kim, Fasman, Baskakov, Qin, Oesch, Conway, Sayer, Rhie, Schatzl, Mead   +56 more
core   +1 more source

Anti- Parkinsonian Drug Estimation by RP-HPLC

Journal of Pharmaceutical Research International, 2021
Aim: The main aim of the current study is to give best and simple method for the estimation of antiparkinsonian drugs named Carbidopa, levodopa and entacapone. Study Design:  Simultaneous estimation of Carbidopa, levodopa and entacapone was performed by using Quadrapumped (SHIMADZU Prominance-i, LC-2030C) RP-HPLC equipped with PDA detector.
Patchala Abhinandana, Rama Rao Nadendla
openaire   +2 more sources

Beyond PEGylation: Archaeal Lipids for Long‐Circulating Liposomes

Advanced NanoBiomed Research, EarlyView.
Archaeal lipid‐based liposomes, particularly those containing caldarchaeol (GDGT), were found to significantly prolong the circulation time of vancomycin in rats, matching the pharmacokinetic properties of PEGylated systems. These findings suggest archaeal lipids as promising non‐PEG excipients for parenteral applications to minimize drug clearance ...
Viktor Sedlmayr, Andreas D. Meid, Eric Mühlberg, Manuel Ben Böhmann, Julian Quehenberger, David Wurm, Mikko Gynther, Oliver Spadiut, Gert Fricker, Philipp Uhl   +9 more
wiley   +1 more source

Human Leukocyte Elastase Triggered Nanoparticles for Targeted Antimicrobial Delivery and Oral Microbial Modulation

Advanced NanoBiomed Research, EarlyView.
This study explores enzyme‐responsive nanoparticles for targeted antimicrobial release in inflamed oral environments. Nanoparticles formed by complexing peptides and chlorhexidine respond to human leukocyte elastase and release chlorhexidine selectively. P7 (ECAAPVCE)‐based formulations show optimal properties and stability, with antimicrobial activity
Mohammed A. Hadis, Ignacio Insua, Farah N. S. Raja, Álvaro Vila, William M. Palin, Michael R. Milward, Paul R. Cooper, Sarah A. Kuehne, Owen Addison, Paco Fernandez‐Trillo   +9 more
wiley   +1 more source

CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES

Química Nova, 2016
Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides ...
Carla Grazieli Azevedo da Silva, Carla Beatriz Grespan Bottoli, Carol H. Collins   +2 more
doaj   +1 more source