Results 31 to 40 of about 2,193 (173)

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

The FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami, Tomoki Kawase, Taira Uehara, Haruhiko Sakuraba, Tomohiko Matsuzawa, Junji Hayashi, Toshihisa Ohshima   +6 more
wiley   +1 more source

Unraveling the active site cover of coproheme decarboxylase from Listeria monocytogenes

The FEBS Journal, EarlyView.
During heme biosynthesis in Gram‐positive bacteria, coproheme decarboxylase (ChdC) catalyzes the conversion of four‐propionate substrate coproheme to the two propionate product heme b. Its active site is universally covered by a flexible linking loop. This study identifies an important histidine residue, which stabilizes the loop in a ChdC homolog.
Nikolaus Falb, Dominik Hörtnagel, Chris Oostenbrink, Paul G. Furtmüller, Stefan Hofbauer   +4 more
wiley   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source

Radial Basis Point Interpolation Method with Reordering Gauss Domains for 2D Plane Problems

Journal of Applied Mathematics, 2014
We present novel Gauss integration schemes with radial basis point interpolation method (RPIM). These techniques define new Gauss integration scheme, researching Gauss points (RGD), and reconstructing Gauss domain (RGD), respectively.
Shi-Chao Yi, Fu-jun Chen, Lin-Quan Yao
doaj   +1 more source

Meshless Analysis of an Embankment Using Local Galerkin Radial Point Interpolation Method (LGRPIM)

Communications, 2013
The paper deals with use of the meshless method for slope stability analysis. There are many formulations of the meshless methods. The article presents the Local Galerkin Radial Point Interpolation method (LGRPIM) - local weak formulation of the ...
Juraj Muzik, Karel Kovarik, Dana Sitanyiova   +2 more
doaj   +1 more source

Analysis of the heterogenous structural states of the hexameric ATPase PilU of the type IV pili from Vibrio cholerae

Protein Science, Volume 35, Issue 6, June 2026.
Abstract Type IV pili (T4P) mediate surface motility, host interactions, and DNA uptake through cycles of extension and retraction. While the primary retraction ATPase PilT has been extensively characterized, its homolog PilU remains less well understood despite being demonstrated as a PilT‐dependent retraction ATPase.
Yirui Guo, Shantanu Shukla, George Minasov, Nicole L. Inniss, Thomas Klose, Valerie L. Tokars, Alfonso Mondragón, Zbyszek Otwinowski, Dominika Borek, Karla J. F. Satchell   +9 more
wiley   +1 more source

3D Global Weak-Form Mesh-Free Method for Acoustic Attenuation Analysis of Expansion Chambers

Shock and Vibration, 2020
In order to avoid the dependence of mesh method on grids, a 3D global weak-form mesh-free method (MFM) is applied to study the three-dimensional acoustic characteristics of silencers.
Chun-Hui Fang, Cheng-Yang Liu, Zhi Fang
doaj   +1 more source

Kinetic and structural characterisation of domain‐specific angiotensin I‐converting enzyme inhibition by captopril, rentiapril and zofenoprilat

The FEBS Journal, Volume 293, Issue 11, Page 3399-3414, June 2026.
Angiotensin I‐converting enzyme (ACE) contains two catalytic domains (nACE and cACE) and is a key therapeutic target for hypertension and cardiovascular disease. Current ACE inhibitors (ACEi) nonselectively inhibit both domains, causing adverse effects. Selective inhibition requires an understanding of domain‐specific binding.
Kyle S. Gregory, Vinasha Ramasamy, Edward D. Sturrock, K. Ravi Acharya   +3 more
wiley   +1 more source

Characteristic-Based Split Meshless Solution for Couette Flow

Civil and Environmental Engineering, 2014
The paper deals with use of the meshless method for incompressible fluid flow analysis. There are many formulations of the meshless methods. The article presents the Meshless Local Petrov-Galerkin method (MLPG) - local weak formulation of the Navier ...
Mužík Juraj
doaj   +1 more source

Structural and functional insights into TBC1D17 highlight the importance of the previously uncharacterized Rab‐binding domain

Protein Science, Volume 35, Issue 5, May 2026.
Abstract TBC (Tre2/Bub2/Cdc16) domain‐containing proteins constitute the widespread family of GTPase‐activating proteins (GAPs). They interact with the Rab superfamily of small GTPases, stimulate GTP hydrolysis, and regulate vesicle trafficking. TBC1D17, involved in Shiga toxin trafficking, autophagy and glucose metabolism regulation, constitutes an ...
Dominika Nielipińska, Marta Orlikowska, Maciej Nielipiński, Bartosz Sekuła, Katarzyna M. Błażewska, Edyta Gendaszewska‐Darmach, Agnieszka J. Pietrzyk‐Brzezińska   +6 more
wiley   +1 more source