The 5′ end of the 18S rRNA can be positioned from within the mature rRNA [PDF]
In yeast, the 5' end of the mature 18S rRNA is generated by endonucleolytic cleavage at site A1, the position of which is specified by two distinct signals. An evolutionarily conserved sequence immediately upstream of the cleavage site has previously been shown to constitute one of these signals.
Sharma, K, Venema, J, Tollervey, D
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rRNA gene restriction patterns as a characterization tool for Lactobacillus sake strains producing ropy slime [PDF]
http://www.elsevier.nl/locate/01681605The rRNA gene restriction patterns (ribotypes) of 69 ropy slime producing Lactobacillus sake strains isolated mainly from vacuum-packaged meat products of ten meat plants were determined.
Björkroth, Johanna +3 more
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Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules. [PDF]
PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes.
James E McDonald +8 more
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The Bowen–Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Psi1191 in yeast 18S rRNA [PDF]
The Nep1 (Emg1) SPOUT-class methyltransferase is an essential ribosome assembly factor and the human Bowen–Conradi syndrome (BCS) is caused by a specific Nep1D86G mutation. We recently showed in vitro that Methanocaldococcus jannaschii Nep1 is a sequence-
Valeska Schilling +27 more
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Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing [PDF]
Many analyses have examined subnucleolar structures in eukaryotic cells, but the relationship between morphological structures, pre-rRNA processing, and ribosomal particle assembly has remained unclear.
Tollervey, D +13 more
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Complete mitochondrial genome sequence of Black Bengal goat (Capra hircus)
The Black Bengal goat (Capra hircus), is a native breed found in Bangladesh, popular due to its economic contribution. Here, we report the complete mitochondrial genome sequence of Black Bengal goat.
Amam Siddiki +12 more
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Molecular biological methods for studying the gut microbiota : the EU human gut flora project [PDF]
Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies ...
Vos, W.M., de +5 more
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The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy [PDF]
The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes.
Probert, Ian +35 more
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Regulatory Aspects of rRNA Modification and Pre-rRNA Processing
The major types of posttranscriptional modification are the isomerization of uracil to pseudouridine, 2′-O-methylation of the ribose moieties and base methylation. In addition to their roles in pre-rRNA modification, both Dim1p and Cbf5p are required for pre-rRNA processing, and Pet56p is required for synthesis of mitochondrial large ribosomal subunits.
Lafontaine, Denis, Tollervey, D
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Identification of a structural motif of 23S rRNA interacting with 5S rRNA
To identify RNA motifs interacting with 5S rRNA, a systematic evolution of ligands by exponential enrichment experiment was applied. Some of the resulting RNA aptamers contained a consensus sequence similar to the sequence in the loop region of helix 89 of 23S rRNA.
Ko, J +3 more
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