Results 101 to 110 of about 37,763 (207)

Noroviruses Surrogate Detection Using Loop-Mediated Isothermal Amplification (LAMP), Conventional RT-PCR and Quantitative RT-PCR in Sg. Tekala and Sg. Gabai Stream Water

MATEC Web of Conferences, 2017
A preliminary study was carried out in order to evaluate a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detecting Noroviruses (NoV) RNA surrogates as external standard for NoV.The detection limit of the NoV RT-LAMP ...
Khairuddin Dzulaikha, Mohd Yusof Nurul Yuziana, Jani Jan Maizatulriah, Ab. Wahid Marfiah   +3 more
doaj   +1 more source

Detection of each DENV serotype by RT-LAMP [PDF]

, 2018
Background 4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences.
Bakheit, Mohammed, Bekaert, Michaël, Duong, Veasna, Dussart, Philippe, Fall, Cheikh, Faye, Oumar, Frischmann, Sieghard, Harold, Graham, Lambrechts, Louis, Lopez-Jimena, Benjamin, Patel, Pranav, Sall, Amadou Alpha, Simon-Loriere, Etienne, Weidmann, Manfred   +13 more
core   +6 more sources

RT-LAMP for BYV detection v1

This protocol describes the process of detecting beet yellows virus (BYV) in plant material utilising a quick and easy colorimetric assay. The starting point is total RNA extracted from the material. The positive results are shown in yellow color, while negative samples stay bright pink - an example of these results is provided at the end of the ...
Marta Niedzicka, Stephen Byrne
openaire   +1 more source

RT-LAMP AND CRISPR APPLICATIONS IN COVID-19 DETECTION

, 2022
SARS-CoV-2, more commonly known as COVID-19, is a novel coronavirus that has spread on a global scale since its emergence in late 2019. The ongoing pandemic fueled researchers to study new COVID-19 detection methods that are more efficient and accurate alongside new mutations that are coming to light.
Tran, Vivian, Chan, Steven-Jethro, Touserkani, Nicholette, Chanchani, Aryaa, Huynh, Thao, Muwonge, Haroon   +5 more
openaire   +1 more source

Nanomolecular Magnetic Probe for Colorimetric RT-LAMP of Viral RNA

Biointerface Research in Applied Chemistry, 2022
Amplification of the RNA from the Covid-19 virus is considered the main objective of the molecular diagnosis of SARS-Cov-2. However, the use of target-based amplification methods such as polymerase chain reaction requires a step to convert the RNA of the Covid-19 virus into a DNA template to lead to amplification.
openaire   +1 more source

Rapid, visual, label-based biosensor platform for identification of hepatitis C virus in clinical applications

BMC Microbiology
Objectives In the current study, for the first time, we reported a novel HCV molecular diagnostic approach termed reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticles-based lateral flow biosensor (RT-LAMP ...
Yuanfang Shi, Qingxue Zhou, Shilei Dong, Qi Zhao, Xue Wu, Peng Yang, Xiaoyan Zeng, Xinggui Yang, Yan Tan, Xinhua Luo, Zhenghua Xiao, Xu Chen   +11 more
doaj   +1 more source

Cross comparison of alternative diagnostic protocols including substitution to the clinical sample, RNA extraction method and nucleic acid amplification technology for COVID-19 diagnosis

Frontiers in Molecular Biosciences
Backgroundthe gold-standard diagnostic protocol (GSDP) for COVID-19 consists of a nasopharyngeal swab (NPS) sample processed through traditional RNA extraction (TRE) and amplified with retrotranscription quantitative polymerase chain reaction (RT-qPCR ...
Ismael Segura-Ulate, Navilla Apú, Bernal Cortés, Jordi Querol-Audi, Jordi Querol-Audi, Yamitzel Zaldívar, Carlos Alexander Ortega, Fernando Flores-Mora, Andrés Gatica-Arias, Andrés Gatica-Arias, Germán Madrigal-Redondo   +10 more
doaj   +1 more source

Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection of novel avian influenza A (H7N9) virus [PDF]

, 2014
BACKGROUND: The emerged human infection with avian influenza A (H7N9) virus in China since 2013 has aroused global concerns. There is great demand for simple and rapid diagnostic method for early detection of H7N9 to provide timely treatment and disease ...
Cheng-Feng Qin, E-De Qin, Jing Li, Juan Liu, Qing-Gong Nian, Qing-Yu Zhu, Shun-Ya Zhu, Tao Jiang, Xiao-Feng Li, Yi Hu, Yong-Qiang Deng, Yu Zhang   +11 more
core   +1 more source

Comparison of Elva Diagnostic SARS-CoV-2 Saliva Nucleic Acid Test Kit with RT-qPCR and Rapid Antigen Tests in COVID-19 Patients

Journal of Pure and Applied Microbiology
The practical application of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) technique has been confirmed in diagnosing different viral infections. Nevertheless, its accuracy in identifying SARS-CoV-2, particularly in practical
Yudi Agustinus Allositandi, Hartono Kahar, Fauqa Arinil Aulia   +2 more
doaj   +1 more source

Rapid, sensitive and effective diagnostic tools for foot-and-mouth disease virus in Africa

Onderstepoort Journal of Veterinary Research, 2014
Speed is paramount in the diagnosis of highly infectious diseases, such as foot-and-mouth disease (FMD), as well as for emerging diseases; however, simplicity is required if a test is to be deployed in the field.
Christopher J. Kasanga, Wataru Yamazaki, Valerie Mioulet, Donald P. King, Misheck Mulumba, Ezekia Ranga, Jimis Deve, Cornelius Mundia, Patrick Chikungwa, Laureta Joao, Philemon N. Wambura, Mark M. Rweyemamu   +11 more
doaj   +1 more source