Tissue culture and next-generation sequencing: A combined approach for detecting yam (Dioscorea spp.) viruses [PDF]
, 2018 In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that ...
Bömer, Moritz +7 more
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Asymmetric Membrane for Digital Detection of Single Bacteria in Milliliters of Complex Water Samples [PDF]
, 2018 In this work, we introduce an asymmetric membrane as a simple and robust nanofluidic platform for digital detection of single pathogenic bacteria directly in 10 mL of unprocessed environmental water samples. The asymmetric membrane, consisting of uniform
Hoffmann, Michael R. +5 more
core +4 more sources
RT-LAMP for Potato Virus Y detection v1
A rapid test was developed primarily to support rapid identification of Potato Virus Y in aphid or crop samples. A colorimetric RT-LAMP assay was developed to detect the presence of Potato Virus Y (PVY).
Md Munir Mostafiz +2 more
openaire +1 more source
Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques [PDF]
The persistence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) on substrates, and the impact of fomites on Coronavirus Disease 19 (COVID-19) transmission, is until now, widely discussed.
Caligaris, Laura +9 more
core +1 more source
Rapid detection of newly isolated Tembusu-related Flavivirus by reverse-transcription loop-mediated isothermal amplification assay [PDF]
, 2011 Background From April 2010 to January 2011, a severe new viral disease had devastated most duck-farming regions in China. This disease affected not only laying ducks but also meat ducks, causing huge economic losses for the poultry industry.
Li, Yufeng +9 more
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Development of reverse transcription loop-mediated isothermal amplification-based assay for rapid and specific detection of human fungal pathogen, Candida auris [PDF]
Current Medical MycologyBackground and Purpose: Due to the ability of Candida auris, a multidrug-resistant human fungal pathogen, to colonize the skin and hospital surfaces, it is pertinent to control its nosocomial outbreaks through rapid diagnosis.
Ankush Kaushik +2 more
doaj +1 more source
Point of care tests for tuberculosis (TB) : economic analysis report [PDF]
A number of clinical/disease areas have been prioritised by the TSB and DH for the DIIA Innovation Platform. To support commissioning of technology developments in the area of tuberculosis detection in humans, a scoping review has been undertaken to help
Osipenko, Leeza, Szczepura, Ala
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Molecular Techniques for the Detection of Organisms in Aquatic Environments, with Emphasis on Harmful Algal Bloom Species. [PDF]
, 2017 Molecular techniques to detect organisms in aquatic ecosystems are being gradually considered as an attractive alternative to standard laboratory methods.
Medlin, LK, Orazco, J
core +1 more source
, 2015 The Central Marine Fisheries Research Institute (CMFRI) established in 1947, has been carrying out pioneering research and development work in the arena of marine fisheries with the objectives of ensuring fisheries resource, ecosystem and livelihood
Narayanakumar, R +3 more
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Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay [PDF]
, 2018 Background Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets.
10359995 +37 more
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