Results 11 to 20 of about 37,268 (281)
Homebrew reagents for low cost RT-LAMP [PDF]
Journal of Biomolecular Techniques : JBT, 2021 ABSTRACTRT-LAMP (reverse transcription - Loop-mediated isothermal amplification) has gained popularity for the detection of SARS-CoV-2. The high specificity, sensitivity, simple protocols and potential to deliver results without the use of expensive equipment has made it an attractive alternative to RT-PCR.
Matute, Tamara +10 more
openaire +4 more sources
Sample Treatment with Trypsin for RT-LAMP COVID-19 Diagnosis. [PDF]
Biology (Basel), 2023 The SARS-CoV-2 coronavirus is responsible for the COVID-19 pandemic resulting in a global health emergency. Given its rapid spread and high number of infected individuals, a diagnostic tool for a rapid, simple, and cost-effective detection was essential.
García-Sorribes S +13 more
europepmc +5 more sources
LAMP/RT-LAMP Buffer protocol v1 [PDF]
, 2021 This protocol describes the preparation of reaction buffer for homemade LAMP / RT-LAMP reactions which involves the use of home-brewed BstLF and MMLV enzymes. It also contains the instructions to prepare the enzyme storage buffers. Home-brewed enzymes information: BstLF polymerase: expression and purification protocol. expression plasmid sequence.
Tamara Matute +2 more
openaire +1 more source
LAMP/RT-LAMP Reaction protocol v1 [PDF]
, 2021 This protocol describes how to perform LAMP / RT-LAMP reactions with home-made reagents, which means home-made buffers and positive controls, together with home-brewed BstLF and MMLV enzymes. Home-brewed enzymes information: BstLF polymerase: expression and purification protocol. expression plasmid sequence.
Tamara Matute +2 more
openaire +1 more source
Low Cost LAMP and RT-LAMP v1 [PDF]
, 2021 This protocol collection specifies how to carry out LAMP or RT-LAMP (reverse transcription - Loop-mediated isothermal amplification) reactions with homemade reagents and home-brewed enzymes. It describes how to prepare all the solutions and buffers to perform the reactions.
Tamara Matute +2 more
openaire +1 more source
RT‐LAMP for rapid diagnosis of coronavirus SARS‐CoV‐2 [PDF]
Microbial Biotechnology, 2020 Summary The pandemic coronavirus SARS‐CoV‐2 in the world has caused a large infected population suffering from COVID‐19. To curb the spreading of the virus, WHO urgently demanded an extension of screening and testing; thus, a rapid and simple diagnostic method is needed.
Huang, Wei E. +13 more
openaire +4 more sources
LAMP/RT-LAMP COVID positive control v1 [PDF]
, 2021 This protocol explains how to prepare dsDNA and ssRNA target positive control for LAMP and RT-LAMP reactions. Particularly it describes the pipeline for SARS-Cov-2 Nucleocapsid (N) gene positive control which is included in the ReClone collection. The general procedure involves amplifying the gene of interest with primers which add T7 promoter and T7 ...
Anibal Arce Medina +3 more
openaire +1 more source
A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening [PDF]
Biosensors, 2021 The COVID-19 pandemic has changed people’s lives and has brought society to a sudden standstill, with lockdowns and social distancing as the preferred preventative measures. To lift these measurements and reduce society’s burden, developing an easy-to-use, rapid, and portable system to detect SARS-CoV-2 is mandatory.
Meysam Rezaei +6 more
openaire +3 more sources
Electromechanical RT-LAMP device for portable SARS-CoV-2 detection
Talanta, 2023 Rapid point-of-care tests for infectious diseases are essential, especially in pandemic conditions. We have developed a point-of-care electromechanical device to detect SARS-CoV-2 viral RNA using the reverse-transcription loop-mediated isothermal amplification (RT-LAMP) principle.
E. Alperay Tarim +5 more
openaire +4 more sources
SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay [PDF]
Wellcome Open Research, 2021 The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a ...
Michael D. Buck +38 more
openaire +4 more sources