Results 41 to 50 of about 565,475 (306)

Specific quantification of inducible HIV-1 reservoir by RT-LAMP. [PDF]

Commun Med (Lond)
Abstract Background Strategies toward HIV-1 cure aim to clear, inactivate, reduce, or immunologically control the virus from a pool of latently infected cells such that combination antiretroviral therapy (cART) can be safely interrupted. In order to assess the impact of any putative curative interventions on the size and
Hossain T, Lungu C, de Schrijver S, Kuali M, Crespo R, Reddy N, Ngubane A, Kan TW, Reddy K, Rao S, Palstra RJ, Madlala P, Ndung'u T, Mahmoudi T.   +13 more
europepmc   +4 more sources

RT-LAMP performance compared to RT-qPCR on nasal swabs.

, 2022
(A) Nasal RT-qPCR results are stratified by the Ct value of the paired nasopharyngeal swab. The table below the graph summarizes percent agreement and 95% confidence intervals at different Ct thresholds.
Anthony Price (9674439), Chelsey A. Smith (1674598), Kathleen M. Schmeler (6308108), Megan M. Chang (10284661), Akshaya Santhanaraj (12152425), Sai Paul (4012577), Ellen Baker (12152431), Christopher Goh (2034826), Kathryn A. Kundrod (10284664), Mary E. Natoli (2095321), Karen W. Eldin (12152428), Keyur P. Patel (3818284), Rebecca Richards-Kortum (44484), Dereq Ogoe (12152422)   +13 more
core   +1 more source

ナイジェリア南東部および中南部におけるラッサウイルス検出のためのRT-LAMPアッセイの開発 [PDF]

, 2020
Lassa virus (LASV) causes Lassa fever (LF), a viral hemorrhagic fever endemic in West Africa. LASV strains are clustered into six lineages according to their geographic location. To confirm a diagnosis of LF, a laboratory test is required.
Pemba, Christelle Mawonga, Pemba, Mawonga Christelle   +1 more
core   +1 more source

LAMP/RT-LAMP COVID positive control v1 [PDF]

, 2021
This protocol explains how to prepare dsDNA and ssRNA target positive control for LAMP and RT-LAMP reactions. Particularly it describes the pipeline for SARS-Cov-2 Nucleocapsid (N) gene positive control which is included in the ReClone collection. The general procedure involves amplifying the gene of interest with primers which add T7 promoter and T7 ...
Anibal Arce Medina, Isaac Núñez, Tamara Matute, Fernan Federici   +3 more
openaire   +2 more sources

RT-LAMP primer sets evaluation.

, 2023
(A) Mapping of RT-LAMP N1 primer set that targets a region in the nucleocapsid (N) gene. Colored boxes represent the open reading frame (ORF) encoding N protein of SARS-CoV-2. (B) RT-LAMP reactions with N1 primer set. The figure shows the electrophoretic
Luis Gabriel Brieba (14510273), Roberto Ruiz-Medrano (510507), Rosalía Lira-Carmona (510504), Eduardo Ferat-Osorio (14510276), Beatriz Xoconostle-Cázares (510503), Berenice Calderón-Pérez (510502), Brenda Yazmín Vargas-Hernández (12027053), Leandro Alberto Núñez-Muñoz (12027050), Constantino López-Macías (2503060), José Abrahán Ramírez-Pool (12027047), Rogelio González-González (8283537), Diana Angélica Tapia-Sidas (14510270)   +11 more
core   +1 more source

SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay [PDF]

Wellcome Open Research, 2021
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a ...
Michael D. Buck, Enzo Z. Poirier, Ana Cardoso, Bruno Frederico, Johnathan Canton, Sam Barrell, Rupert Beale, Richard Byrne, Simon Caidan, Margaret Crawford, Laura Cubitt, Sonia Gandhi, Robert Goldstone, Paul R. Grant, Kiran Gulati, Steve Hindmarsh, Michael Howell, Michael Hubank, Rachael Instrell, Ming Jiang, George Kassiotis, Wei-Ting Lu, James I. MacRae, Iana Martini, Davin Miller, David Moore, Eleni Nastouli, Jerome Nicod, Luke Nightingale, Jessica Olsen, Amin Oomatia, Nicola O'Reilly, Anett Rideg, Ok-Ryul Song, Amy Strange, Charles Swanton, Samra Turajlic, Mary Wu, Caetano Reis e Sousa   +38 more
openaire   +4 more sources

Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats [PDF]

PeerJ, 2019
Background Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect HTNV and SEOV ...
Xin Sui, Xu Zhang, Dongliang Fei, Zhen Zhang, Mingxiao Ma   +4 more
doaj   +2 more sources

Primer sequences used in RT-LAMP and RT-qPCR.

, 2022
Primer sequences used in RT-LAMP and RT-qPCR.
Yoric Yali-Assy-Oyamli (14204806), Jiro Yasuda (32563), Haruka Abe (3174081), Rodrigue Bikangui (5851262), Yuri Ushijima (223591), Bertrand Lell (224978), Ayola A. Adegnika (9080248), Rokusuke Yoshikawa (401202), Georgelin Nguema Ondo (14204800), Ayong Moure (14204803)   +9 more
core   +1 more source

Self-Sampled Gargle Water Direct RT-LAMP as a Screening Method for the Detection of SARS-CoV-2 Infections

Diagnostics, 2022
We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples.
Skaiste Arbaciauskaite, Pouya Babakhani, Natalia Sandetskaya, Dalius Vitkus, Ligita Jancoriene, Dovile Karosiene, Dovile Karciauskaite, Birute Zablockiene, Dirk Kuhlmeier   +8 more
doaj   +1 more source

Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces. [PDF]

Viruses
The wide host range, potential lethality, and zoonotic potential of SARS-CoV-2 infection in animals highlights the need for additional surveillance strategies. We validated a commercial, pH-based, colorimetric RT-LAMP assay for the detection of SARS-CoV-2 RNA in animal feces. The comparator assay was rRT-PCR. The limit of detection of the RT-LAMP assay
Pepper A, Kayastha S, Miller M, Guag J, Tkachenko A, Allender M, Terio K, Wang L.   +7 more
europepmc   +4 more sources