Results 61 to 70 of about 565,475 (306)

A fully integrated duplex RT-LAMP device for the detection of viral infections

open access: yesBiomedical Microdevices, 2023
AbstractRespiratory viruses can cause epidemics or pandemics, which are worldwide outbreaks of disease. The severity of these events varies depending on the virus, its characteristics, along with environmental factors. The frequency of epidemics and pandemics caused by respiratory viruses is difficult to predict, but the potential severity of such ...
Nicolas Mytzka   +4 more
openaire   +2 more sources

Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases

open access: yesBMC Infectious Diseases, 2009
Background In the global eradication program for poliomyelitis, the laboratory diagnosis plays a critical role by isolating poliovirus (PV) from the stool samples of acute flaccid paralysis (AFP) cases. In this study, we developed a reverse transcription-
Wakita Takaji   +6 more
doaj   +1 more source

Specificity of SVA RT-LAMP-LFD detection method.

open access: yes, 2019
M: 2000 bp DNA marker; lanes 1 to 10 are SVA, FMDV, SVDV, VSV, PDCoV, PEDV, TGEV, PRRSV, CSFV and negative control (N); A): specificity of RT-LAMP agarose gel electrophoresis assay; B): specificity of calcein RT-LAMP assay; and C): specificity of RT-LAMP-
Shuaifei Xu (5741528)   +9 more
core   +1 more source

Reactivities and specificities of RT-LAMP-BART assays detecting RdRp gene and the L452R spike mutation of SARS-CoV-2.

open access: yes, 2022
Reactivities and specificities of RT-LAMP-BART assays detecting RdRp gene and the L452R spike mutation of SARS-CoV-2.
Shinnosuke Ando (12269827)   +11 more
core   +1 more source

Colorimetric LAMP/RT-LAMP Protocol v1

open access: yes, 2022
This protocol describes how to perform colorimetric LAMP / RT-LAMP reactions with homemade buffers, together with home-brewed BstLF and MMLV enzymes. It is based on previous protocols of our lab (see LAMP/RT-LAMP Reaction protocol), but since two of the most common indicators used in colorimetric LAMP (phenol red and neutral red) require weakly ...
openaire   +1 more source

A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing

open access: yesFrontiers in Molecular Biosciences, 2022
RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use.
Max J. Kellner   +31 more
doaj   +1 more source

Sensitivity of SVA RT-LAMP-LFD detection method.

open access: yes, 2019
1: 4.56x10-1 ng/μL; 2: 4.56x10-2 ng/μL; 3: 4.56x10-3 ng/μL; 4: 4.56x10-4 ng/μL; 5: 4.56x10-5 ng/μL; 6: 4.56x10-6 ng/μL; 7: 4.56x10-7 ng/μL; 8: 4.56x10-8 ng/μL; and 9: 4.56x10-9 ng/μL; M: 2000 bp DNA marker; A): sensitivity of RT-LAMP agarose gel ...
Shuaifei Xu (5741528)   +9 more
core   +1 more source

Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome. [PDF]

open access: yes, 2018
A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed.
Michaël Bekaert   +26 more
core   +2 more sources

Primer information for RT-LAMP of HpeVs.

open access: yes, 2021
Primer information for RT-LAMP of HpeVs.
Tadafumi Yokoyama (811274)   +6 more
core   +1 more source

Blood RNA Biomarker Signatures for Early Diagnosis and Prognosis in Ischemic and Hemorrhagic Stroke: The IBIS‐CT1 Study

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the expression of nine blood RNA biomarkers in a clinical trial based on genes previously identified in an experimental monkey model of stroke for diagnosis feasibility and prognostication. Methods IBIS‐CT1 was a prospective longitudinal study enrolling patients with ischemic stroke (IS) or intracerebral hemorrhage (ICH ...
Salomé Retailleau   +11 more
wiley   +1 more source

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