Results 51 to 60 of about 565,475 (306)

Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay

Biosensors, 2023
This study focuses on three key aspects: (a) crude throat swab samples in a viral transport medium (VTM) as templates for RT-LAMP reactions; (b) a biotinylated DNA probe with enhanced specificity for LFA readouts; and (c) a digital semi-quantification of
Saloni Agarwal, Mojdeh Hamidizadeh, Frank F. Bier   +2 more
doaj   +1 more source

Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification

Virology Journal, 2011
For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method.
Zhang Zong-Ying, Liu Xiao-Jun, Li Da-Wei, Yu Jia-Lin, Han Cheng-Gui   +4 more
doaj   +1 more source

Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis

Scientific Reports, 2023
The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. However, a high false-positive rate due to misamplification is one of the major limitations.
Galyah Alhamid, Huseyin Tombuloglu, Ebtesam Al-Suhaimi   +2 more
doaj   +1 more source

RT-LAMP and RT-PCR primer sequences.

, 2019
RT-LAMP and RT-PCR primer sequences.
Michele Tchokwansi (6635822), Léopold Lehman (6635843), Lawrence Ayong (309445), Emmanuel Elanga-Ndille (6635840), Umaru Banlanjo (6635825), Sylvie Kemleu (3337653), Balotin Fogang (6635831), Estelle Essangui (3337656), Carole Else Eboumbou Moukoko (6411722), Niels Nguedia (6635819), Christiane Donkeu (3337671), Marie Biabi (6635834), Glwadys Cheteug (6635837), Franklin Maloba (6635828)   +13 more
core   +1 more source

Rapid diagnostics of genital herpes by loop-mediated isothermal amplification method with fluorescent detection

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Introduction. Due to the high clinical significance of herpesvirus diseases, the searching of fast and effective methods for their diagnosis remains relevant.The aim of the study was to evaluate the diagnostic efficiency of the loop-mediated isothermal ...
D. I. Smirnova, O. A. Petrusha, A. V. Gracheva, E. A. Volynskaya, V. V. Zverev, E. B. Faizuloev   +5 more
doaj   +1 more source

RT-LAMP primer sequences.

, 2016
RT-LAMP primer sequences.
Dylan Guelig (666473), Bernard Melingui (3337662), Lawrence Ayong (309445), Paul LaBarre (221735), Sylvie Kemleu (3337653), Robert Burton (802746), Carole Eboumbou Moukoko (3337668), Steven Diesburg (802744), Abas Mouliom (3337665), Annie Epote (3337659), Estelle Essangui (3337656), Christiane Donkeu (3337671), Jeanne Manga (3337650), Gaëtan Texier (546523)   +13 more
core   +1 more source

Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?

Biosensors, 2022
Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to
Cassidy Mannier, Jeong-Yeol Yoon
doaj   +1 more source

Establishment of SVA RT-LAMP-LFD detection method.

, 2019
A): RT-LAMP with gel electrophoresis detection: M: 2000 bp DNA marker, lane 1 for positive template (P), lane 2 for negative control (N); B): calcein RT-LAMP detection method: tube 1 for positive template (P), tube 2 for negative control (N); C): RT-LAMP-
Shuaifei Xu (5741528), Li Yu (81651), Guangbin Si (6659090), Xia Zhou (291729), Dongsheng He (1295079), Jingfeng Xiong (6659087), Jinhui Li (625245), Bowen Liu (2009599), Jian Shi (141852), Weifang Liang (6659084)   +9 more
core   +1 more source

Developing RT-LAMP assays for rapid diagnosis of SARS-CoV-2 in saliva

EBioMedicine, 2022
Summary: Background: The coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has killed millions of people worldwide. The current crisis has created an unprecedented demand for rapid test of SARS-CoV-2 infection. Methods: Reverse transcription loop-
Xin Huang, Gongyu Tang, Nahed Ismail, Xiaowei Wang   +3 more
doaj   +1 more source

The results of RT-LAMP using different primer sets.

, 2021
The RT-LAMP experiments were conducted using Sets A, B, and C. All sets show the same results. Neg, negative; Pos, positive; RT-LAMP, reverse transcription loop-mediated isothermal amplification. (TIF)
Tadafumi Yokoyama (811274), Eri Nariai (8938511), Taizo Wada (3415721), Sanae Kuramoto (8938508), Naotoshi Sugimoto (301261), Yuko Tasaki (11767782), Natsumi Inoue (3415712)   +6 more
core   +1 more source