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Rapid and sensitive detection of novel avian-origin influenza A (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device. [PDF]
A severe disease in humans caused by a novel avian-origin influenza A (H7N9) virus emerged in China recently, which has caused at least 128 cases and 26 deaths.
Yiyue Ge +10 more
doaj +1 more source
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak. Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a ...
Rokusuke Yoshikawa +5 more
doaj +1 more source
Sample Treatment with Trypsin for RT-LAMP COVID-19 Diagnosis
The SARS-CoV-2 coronavirus is responsible for the COVID-19 pandemic resulting in a global health emergency. Given its rapid spread and high number of infected individuals, a diagnostic tool for a rapid, simple, and cost-effective detection was essential.
Soraya García-Sorribes +13 more
openaire +4 more sources
Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples
During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse ...
Konrad Herbst +5 more
doaj +1 more source
A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening [PDF]
The COVID-19 pandemic has changed people’s lives and has brought society to a sudden standstill, with lockdowns and social distancing as the preferred preventative measures. To lift these measurements and reduce society’s burden, developing an easy-to-use, rapid, and portable system to detect SARS-CoV-2 is mandatory.
Meysam Rezaei +6 more
openaire +3 more sources
Although the reverse transcription-polymerase chain reaction (RT-PCR) is considered a standard-of-care assay for the laboratory diagnosis of SARS-CoV-2, several limitations of this method have been described.
Alexander Domnich +11 more
doaj +1 more source
RT-PCR versus extraction-less RT-LAMP workflow.
RT-PCR versus extraction-less RT-LAMP workflow.
Arun Manoharan (4622767) +3 more
core +1 more source
Development of a RT-LAMP assay for detection of SARS-CoV-2
Background & objectives The pandemic of SARS-COV-2 began in Wuhan, China in December 2019 and has caused more than 101 million cases worldwide. Diagnostic technologies possessing sensitivity and specificity equivalent to real-time reverse-transcriptase polymerase chain reaction (rRT-PCR) assays are needed to ramp up testing
Nandi, Shyam Sundar +4 more
openaire +2 more sources

