Results 11 to 20 of about 565,475 (306)
Homebrew reagents for low cost RT-LAMP [PDF]
Journal of Biomolecular Techniques : JBT, 2021 ABSTRACT RT-LAMP (reverse transcription - Loop-mediated isothermal amplification) has gained popularity for the detection of SARS-CoV-2. The high specificity, sensitivity, simple protocols and potential to deliver results without the use of expensive equipment has made it an attractive alternative to RT-PCR.
Matute, Tamara +10 more
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Development of RT-LAMP assay for variants of HCV in Pakistan. [PDF]
Braz J MicrobiolMumtaz Z, Saqib S, Yousaf MZ, Haque MFU.
europepmc +3 more sources
RT‐LAMP for rapid diagnosis of coronavirus SARS‐CoV‐2 [PDF]
Microbial Biotechnology, 2020 Summary The pandemic coronavirus SARS‐CoV‐2 in the world has caused a large infected population suffering from COVID‐19. To curb the spreading of the virus, WHO urgently demanded an extension of screening and testing; thus, a rapid and simple diagnostic method is needed.
Huang, Wei E. +13 more
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Low Cost LAMP and RT-LAMP v1 [PDF]
, 2021 This protocol collection specifies how to carry out LAMP or RT-LAMP (reverse transcription - Loop-mediated isothermal amplification) reactions with homemade reagents and home-brewed enzymes. It describes how to prepare all the solutions and buffers to perform the reactions.
Tamara Matute +2 more
openaire +1 more source
Advances in RT-LAMP for COVID-19 testing and diagnosis
Expert Review of Molecular Diagnostics, 2023 The SARS-CoV-2 pandemic, and the subsequent limitations on standard diagnostics, has vastly expanded the user base of Reverse Transcription Loop-mediated isothermal Amplification (RT-LAMP) in fundamental research and development. RT-LAMP has also penetrated commercial markets, with emergency use authorizations for clinical diagnosis.This review ...
Gihoon Choi +2 more
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LAMP/RT-LAMP Reaction protocol v1 [PDF]
, 2021 This protocol describes how to perform LAMP / RT-LAMP reactions with home-made reagents, which means home-made buffers and positive controls, together with home-brewed BstLF and MMLV enzymes. Home-brewed enzymes information: BstLF polymerase: expression and purification protocol. expression plasmid sequence.
Tamara Matute +2 more
openaire +1 more source
A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats. [PDF]
Microbiol Spectr, 2023 ABSTRACT The availability of simple, inexpensive assays for coronavirus (CoV) detection is critical for conducting animal surveillance studies to prevent new zoonotic epidemics. We have previously developed a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for diagnosing coronavirus ...
Chan TT-Y +13 more
europepmc +6 more sources
Development of a Saliva-Optimized RT-LAMP Assay for SARS-CoV-2 [PDF]
Journal of Biomolecular Techniques : JBT, 2021 Abstract Conventional reverse transcription quantitative polymerase chain reaction (RT-qPCR) technology has struggled to fulfill the unprecedented need for diagnostic testing created by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic.
Albert D, Yu +6 more
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LAMP/RT-LAMP Buffer protocol v1 [PDF]
, 2021 This protocol describes the preparation of reaction buffer for homemade LAMP / RT-LAMP reactions which involves the use of home-brewed BstLF and MMLV enzymes. It also contains the instructions to prepare the enzyme storage buffers. Home-brewed enzymes information: BstLF polymerase: expression and purification protocol. expression plasmid sequence.
Tamara Matute +2 more
openaire +1 more source
RT-LAMP as Diagnostic Tool for Influenza—A Virus Detection in Swine
Veterinary Sciences, 2023 Point-of-care diagnostic technologies are becoming more widely available for production species. Here, we describe the application of reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect the matrix (M) gene of influenza A virus in swine (IAV-S).
Suzanna M. Storms +4 more
openaire +3 more sources