RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods [PDF]
Diagnostics, 2021 Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription–quantitative polymerase chain reaction (RT-qPCR).
Kasturi Selvam +6 more
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Specific quantification of inducible HIV-1 reservoir by RT-LAMP. [PDF]
Commun Med (Lond)Abstract Background Strategies toward HIV-1 cure aim to clear, inactivate, reduce, or immunologically control the virus from a pool of latently infected cells such that combination antiretroviral therapy (cART) can be safely interrupted. In order to assess the impact of any putative curative interventions on the size and
Hossain T +13 more
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Advances in RT-LAMP for COVID-19 testing and diagnosis
Expert Review of Molecular Diagnostics, 2023 The SARS-CoV-2 pandemic, and the subsequent limitations on standard diagnostics, has vastly expanded the user base of Reverse Transcription Loop-mediated isothermal Amplification (RT-LAMP) in fundamental research and development. RT-LAMP has also penetrated commercial markets, with emergency use authorizations for clinical diagnosis.This review ...
Gihoon Choi +2 more
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PLoS ONE, 2020 Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV.
Ning Qiao +6 more
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Evaluation of a direct reverse transcription loop-mediated isothermal amplification method without RNA extraction for the detection of human enterovirus 71 subgenotype C4 in nasopharyngeal swab specimens. [PDF]
PLoS ONE, 2012 Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD) worldwide and has been associated with neurological complications which resulted in fatalities during recent outbreak in Asia pacific region.
Kai Nie +10 more
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Annals of Hepatology, 2023 Introduction and Objectives: SARS-CoV-2 active infection diagnosis is currently performed through RT-qPCR. Despite the fact that PCR-based assays can provide results relatively fast, these techniques require capable professionals, specific equipment and ...
Vanessa Duarte da Costa +13 more
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Measures for diagnosing and treating infections by a novel coronavirus responsible for a pneumonia outbreak originating in Wuhan, China. [PDF]
, 2020 On 10 January 2020, a new coronavirus causing a pneumonia outbreak in Wuhan City in central China was denoted as 2019-nCoV by the World Health Organization (WHO).
Du, Lanying +4 more
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Development of a Saliva-Optimized RT-LAMP Assay for SARS-CoV-2 [PDF]
Journal of Biomolecular Techniques : JBT, 2021 AbstractConventional reverse transcription quantitative polymerase chain reaction (RT-qPCR) technology has struggled to fulfill the unprecedented need for diagnostic testing created by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic.
Albert D, Yu +6 more
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Rapid and sensitive detection of novel avian-origin influenza A (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device. [PDF]
PLoS ONE, 2013 A severe disease in humans caused by a novel avian-origin influenza A (H7N9) virus emerged in China recently, which has caused at least 128 cases and 26 deaths.
Yiyue Ge +10 more
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RT-LAMP as Diagnostic Tool for Influenza-A Virus Detection in Swine. [PDF]
Vet Sci, 2023 Point-of-care diagnostic technologies are becoming more widely available for production species. Here, we describe the application of reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect the matrix (M) gene of influenza A virus in swine (IAV-S).
Storms SM +4 more
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