Results 21 to 30 of about 37,268 (281)

Specific quantification of inducible HIV-1 reservoir by RT-LAMP. [PDF]

Commun Med (Lond)
Abstract Background Strategies toward HIV-1 cure aim to clear, inactivate, reduce, or immunologically control the virus from a pool of latently infected cells such that combination antiretroviral therapy (cART) can be safely interrupted. In order to assess the impact of any putative curative interventions on the size and
Hossain T, Lungu C, de Schrijver S, Kuali M, Crespo R, Reddy N, Ngubane A, Kan TW, Reddy K, Rao S, Palstra RJ, Madlala P, Ndung'u T, Mahmoudi T.   +13 more
europepmc   +4 more sources

RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods [PDF]

Diagnostics, 2021
Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription–quantitative polymerase chain reaction (RT-qPCR).
Kasturi Selvam, Mohamad Najib, Muhammad Khalid, Suharni Mohamad, Fahreddin Palaz, Mehmet Ozsoz, Ismail Aziah   +6 more
openaire   +3 more sources

Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR [PDF]

, 2023
Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymer- ase chain reaction (RT-PCR).
ALP, ALPASLAN, Andac, Ayse, Erdem, Murat, Karahan, Gizem, Palaz, Fahreddin, Tozluyurt, Abdullah, Özketen, Ahmet Çağlar, ÜNAL, Serhat   +7 more
core   +1 more source

RT-LAMP as Diagnostic Tool for Influenza-A Virus Detection in Swine. [PDF]

Vet Sci, 2023
Point-of-care diagnostic technologies are becoming more widely available for production species. Here, we describe the application of reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect the matrix (M) gene of influenza A virus in swine (IAV-S).
Storms SM, Shisler J, Nguyen TH, Zuckermann FA, Lowe JF.   +4 more
europepmc   +4 more sources

Evaluation of a saliva molecular point of care for the detection of SARS-CoV-2 in ambulatory care

Scientific Reports, 2021
Rapid identification of SARS-CoV-2-infected individuals is a cornerstone for the control of virus spread. The sensitivity of SARS-CoV-2 RNA detection by RT-PCR is similar in saliva and nasopharyngeal swabs.
Jérôme LeGoff, Solen Kernéis, Caroline Elie, Séverine Mercier-Delarue, Nabil Gastli, Laure Choupeaux, Jacques Fourgeaud, Marie-Laure Alby, Pierre Quentin, Juliette Pavie, Patricia Brazille, Marie Laure Néré, Marine Minier, Audrey Gabassi, Chrystel Leroy, Béatrice Parfait, Jean-Marc Tréluyer, Constance Delaugerre   +17 more
doaj   +1 more source

Evaluation of a direct reverse transcription loop-mediated isothermal amplification method without RNA extraction for the detection of human enterovirus 71 subgenotype C4 in nasopharyngeal swab specimens. [PDF]

PLoS ONE, 2012
Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD) worldwide and has been associated with neurological complications which resulted in fatalities during recent outbreak in Asia pacific region.
Kai Nie, Shun-Xiang Qi, Yong Zhang, Le Luo, Yun Xie, Meng-Jie Yang, Yi Zhang, Jin Li, Hongwei Shen, Qi Li, Xue-Jun Ma   +10 more
doaj   +1 more source

P-14 OPTIMIZATION OF MOLECULAR METHODS FOR SARS-CoV-2 QUALITATIVE DETECTION AND GENOTYPING IN RESPIRATORY SPECIMENS FROM PATIENTS WITH LIVER DISEASE

Annals of Hepatology, 2023
Introduction and Objectives: SARS-CoV-2 active infection diagnosis is currently performed through RT-qPCR. Despite the fact that PCR-based assays can provide results relatively fast, these techniques require capable professionals, specific equipment and ...
Vanessa Duarte da Costa, Alanna Calheiros Santos, Lucas Limas da Silva, Wilian Jean Wiggers, Claudia Alexandra Pontes Ivantes, Danielle Malta Lima, Jeová Keny Baima Colares, Deusilene Souza Vieira Dallacqua, Ana Rita Coimbra Motta-Castro, Vanessa Salete de Paula, Alberto Martín Rivera Dávila, Priscilla Pollo-Flores, Lia Laura Lewis-Ximenez, Livia Melo Villar   +13 more
doaj   +1 more source

Advances in RT-LAMP for COVID-19 testing and diagnosis

Expert Review of Molecular Diagnostics, 2023
The SARS-CoV-2 pandemic, and the subsequent limitations on standard diagnostics, has vastly expanded the user base of Reverse Transcription Loop-mediated isothermal Amplification (RT-LAMP) in fundamental research and development. RT-LAMP has also penetrated commercial markets, with emergency use authorizations for clinical diagnosis.This review ...
Gihoon Choi, Taylor J. Moehling, Robert J. Meagher   +2 more
openaire   +2 more sources

Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay.

PLoS ONE, 2020
Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV.
Ning Qiao, Huijie Dai, Jie Liu, Xiaoping Zhu, Jintang Li, Dezhen Zhang, Yongguang Liu   +6 more
doaj   +1 more source

Compact highly sensitive photothermal RT-LAMP chip for simultaneous multidisease detection. [PDF]

Sci Adv
Developing instant detection systems with disease diagnostic capabilities holds immense importance for remote or resource-limited areas. However, the task of creating these systems—which are simultaneously easy to operate, rapid in detection, and cost-effective—remains a challenge.
Guo W, Tao Y, Yang R, Mao K, Zhou H, Xu M, Sun T, Li X, Shi C, Ge Z, Xue R, Zhou H, Ren Y.   +12 more
europepmc   +3 more sources