Results 51 to 60 of about 37,763 (207)

Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP [PDF]

, 2015
In this paper, we asked if it is possible to identify the best primers and reaction conditions based on improvements in reaction speed when optimizing isothermal reactions.
Ismagilov, Rustem F., Karymov, Mikhail A., Khorosheva, Eugenia M., Selck, David A.   +3 more
core   +2 more sources

Detection of Acute HIV-1 Infection by RT-LAMP

PLOS ONE, 2015
A rapid, cost-effective diagnostic test for the detection of acute HIV-1 infection is highly desired. Isothermal amplification techniques, such as reverse-transcription loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for the development of a rapid nucleic acid amplification test (NAAT) because they are quick ...
Donna L Rudolph, Vickie Sullivan, S Michele Owen, Kelly A Curtis   +3 more
openaire   +4 more sources

Self-Sampled Gargle Water Direct RT-LAMP as a Screening Method for the Detection of SARS-CoV-2 Infections

Diagnostics, 2022
We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples.
Skaiste Arbaciauskaite, Pouya Babakhani, Natalia Sandetskaya, Dalius Vitkus, Ligita Jancoriene, Dovile Karosiene, Dovile Karciauskaite, Birute Zablockiene, Dirk Kuhlmeier   +8 more
doaj   +1 more source

Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification [PDF]

, 2020
Isothermal amplification assays, such as loop-mediated isothermal amplification (LAMP), show great utility for the development of rapid diagnostics for infectious diseases because they have high sensitivity, pathogen-specificity and potential for ...
Barlow, Jacob T., Ismagilov, Rustem F., Jue, Erik, Rolando, Justin C.   +3 more
core   +2 more sources

[RETRACTED] LAMP/RT-LAMP v1

, 2022
[RETRACTED]LAMP/RT-LAMP
openaire   +1 more source

Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons [PDF]

Genome Biology, 2021
Abstract Background Rapid spread of SARS-CoV-2 has led to a global pandemic, resulting in the need for rapid assays to allow diagnosis and prevention of transmission. Reverse transcription-polymerase chain reaction (RT-PCR) provides a gold standard assay for SARS-CoV-2 RNA, but instrument costs
Scott Sherrill-Mix, Young Hwang, Aoife M. Roche, Abigail Glascock, Susan R. Weiss, Yize Li, Leila Haddad, Peter Deraska, Caitlin Monahan, Andrew Kromer, Jevon Graham-Wooten, Louis J. Taylor, Benjamin S. Abella, Arupa Ganguly, Ronald G. Collman, Gregory D. Van Duyne, Frederic D. Bushman   +16 more
openaire   +3 more sources

Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis

Scientific Reports, 2023
The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. However, a high false-positive rate due to misamplification is one of the major limitations.
Galyah Alhamid, Huseyin Tombuloglu, Ebtesam Al-Suhaimi   +2 more
doaj   +1 more source

Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification

Virology Journal, 2011
For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method.
Zhang Zong-Ying, Liu Xiao-Jun, Li Da-Wei, Yu Jia-Lin, Han Cheng-Gui   +4 more
doaj   +1 more source

Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay

Biosensors, 2023
This study focuses on three key aspects: (a) crude throat swab samples in a viral transport medium (VTM) as templates for RT-LAMP reactions; (b) a biotinylated DNA probe with enhanced specificity for LFA readouts; and (c) a digital semi-quantification of
Saloni Agarwal, Mojdeh Hamidizadeh, Frank F. Bier   +2 more
doaj   +1 more source

Mini review: Recent progress in RT-LAMP enabled COVID-19 detection

Sensors and Actuators Reports, 2020
The coronavirus disease 2019 (COVID-19) pandemic has infected millions of people around the globe. The outbreak caused by the novel coronavirus (SARS-CoV-2) poses a great health risk to the public. Therefore, rapid and accurate diagnosis of the virus plays a crucial role in treatment of the disease and saving lives.
Thompson, Dorian, Lei, Yu
openaire   +4 more sources