Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?
Biosensors, 2022 Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to
Cassidy Mannier, Jeong-Yeol Yoon
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Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification [PDF]
, 2011 A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C.
Chen, Hao-tai +3 more
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Development of reverse transcription loop mediated isothermal amplification assay for visual detection of Grapevine fleck virus (GFkV) [PDF]
, 2018 Grapevine fleck virus (GFkV) produces a ubiquitous disease, latent in grapevine causing simple or complex infections with other more dangerous viruses.
Almasi, M. A.
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Developing RT-LAMP assays for rapid diagnosis of SARS-CoV-2 in saliva
EBioMedicine, 2022 Summary: Background: The coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has killed millions of people worldwide. The current crisis has created an unprecedented demand for rapid test of SARS-CoV-2 infection. Methods: Reverse transcription loop-
Xin Huang +3 more
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Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification [PDF]
, 2020 Isothermal amplification assays, such as loop-mediated isothermal amplification (LAMP), show great utility for the development of rapid diagnostics for infectious diseases because they have high sensitivity, pathogen-specificity and potential for ...
Barlow, Jacob T. +3 more
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A simple direct RT-LAMP SARS-CoV-2 saliva diagnostic [PDF]
, 2020 AbstractWidespread, frequent testing is essential for curbing the ongoing COVID-19 pandemic. Because its simplicity makes it ideal for widely distributed, high throughput testing, RT-LAMP provides an attractive alternative to RT-qPCR. However, most RT-LAMP protocols require the purification of RNA, a complex and low-throughput bottleneck that has often
Michael J. Flynn +7 more
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A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing
Frontiers in Molecular Biosciences, 2022 RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use.
Max J. Kellner +31 more
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Advanced Functional Materials, EarlyView.Novel ferrocene derivatives (e.g., FcPhc2) are used as an ultrathin layer hole‐blocking layer, reducing hole injection from the Ag contact. This results in an ultralow noise spectral density of 1.2 × 10−14 A Hz−1/2, and a high specific detectivity of 8.1 × 1012 Jones at −0.5 V.
Eunyoung Hong +16 more
wiley +1 more source
Вопросы вирусологии, 2022 Introduction. Currently, the basis for molecular diagnostics of most infections is the use of reverse transcription polymerase chain reaction (RT-PCR). Technologies based on reverse transcription isothermal loop amplification (RT-LAMP) can be used as an ...
V. G. Akimkin +9 more
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Artificial Synthesis of Conserved Segment S Gene Fragment of Rift Valley Fever Virus and Preliminary Study of Its Reverse Transcription Loop-Mediated Isothermal Amplification Detection Method [PDF]
, 2016 Purpose: To develop a rapid detection method for Rift Valley fever virus (RVFV) diagnosis.Methods: According to the reference sequences of RVFV published in GenBank, nine overlapping polymerase chain reaction (PCR) primers and four specific reverse ...
Hou, Yihong +8 more
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