Results 61 to 70 of about 37,763 (207)
Colorimetric LAMP/RT-LAMP Protocol v1
, 2022 This protocol describes how to perform colorimetric LAMP / RT-LAMP reactions with homemade buffers, together with home-brewed BstLF and MMLV enzymes. It is based on previous protocols of our lab (see LAMP/RT-LAMP Reaction protocol), but since two of the most common indicators used in colorimetric LAMP (phenol red and neutral red) require weakly ...
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Журнал микробиологии, эпидемиологии и иммунобиологии, 2019 Introduction. Due to the high clinical significance of herpesvirus diseases, the searching of fast and effective methods for their diagnosis remains relevant.The aim of the study was to evaluate the diagnostic efficiency of the loop-mediated isothermal ...
D. I. Smirnova +5 more
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Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?
Biosensors, 2022 Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to
Cassidy Mannier, Jeong-Yeol Yoon
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A Field-Tailored Reverse Transcription Loop-Mediated Isothermal Assay for High Sensitivity Detection of Plasmodium falciparum Infections. [PDF]
PLoS ONE, 2016 Highly sensitive and field deployable molecular diagnostic tools are critically needed for detecting submicroscopic, yet transmissible levels of malaria parasites prevalent in malaria endemic countries worldwide.
Sylvie Kemleu +13 more
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BMC Infectious Diseases, 2009 Background In the global eradication program for poliomyelitis, the laboratory diagnosis plays a critical role by isolating poliovirus (PV) from the stool samples of acute flaccid paralysis (AFP) cases. In this study, we developed a reverse transcription-
Wakita Takaji +6 more
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A simple direct RT-LAMP SARS-CoV-2 saliva diagnostic [PDF]
, 2020 AbstractWidespread, frequent testing is essential for curbing the ongoing COVID-19 pandemic. Because its simplicity makes it ideal for widely distributed, high throughput testing, RT-LAMP provides an attractive alternative to RT-qPCR. However, most RT-LAMP protocols require the purification of RNA, a complex and low-throughput bottleneck that has often
Michael J. Flynn +7 more
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A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing
Frontiers in Molecular Biosciences, 2022 RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use.
Max J. Kellner +31 more
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Development of reverse transcription loop mediated isothermal amplification assay for visual detection of Grapevine fleck virus (GFkV) [PDF]
, 2018 Grapevine fleck virus (GFkV) produces a ubiquitous disease, latent in grapevine causing simple or complex infections with other more dangerous viruses.
Almasi, M. A.
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Review Updated Values for Molecular Diagnosis for Highly Pathogenic Avian Influenza Virus [PDF]
, 2012 viruse
Akira Sakurai, Futoshi Shibasaki
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Developing RT-LAMP Assays for Detection of SARS-CoV-2 in Saliva [PDF]
, 2021 AbstractThe coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has killed millions of people worldwide. The current crisis has created an unprecedented demand for rapid test of SARS-CoV-2 infection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a fast and convenient method to amplify and identify the transcripts of a ...
Xin Huang +3 more
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