Results 221 to 230 of about 1,155,913 (246)
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2011
Reverse transcription PCR (RT-PCR) is one of the most important techniques for analyzing RNA abundance. MicroRNAs (miRNAs) are a group of 20- to 24-nucleotide regulatory small RNAs which play an important role in plants and animals. However, the small size of miRNAs makes them difficult to be detected and quantified by conventional RT-PCR techniques ...
Rui Shi +3 more
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Reverse transcription PCR (RT-PCR) is one of the most important techniques for analyzing RNA abundance. MicroRNAs (miRNAs) are a group of 20- to 24-nucleotide regulatory small RNAs which play an important role in plants and animals. However, the small size of miRNAs makes them difficult to be detected and quantified by conventional RT-PCR techniques ...
Rui Shi +3 more
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2003
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
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Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
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2010
Part I: The RT-PCR Detective: Hunting Down the Best Method 1. Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology Wolfgang Mann and Thomas Haaf 2. Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers 3. Transcriptome Profiling of Host-Microbe
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Part I: The RT-PCR Detective: Hunting Down the Best Method 1. Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology Wolfgang Mann and Thomas Haaf 2. Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers 3. Transcriptome Profiling of Host-Microbe
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2002
Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small ...
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Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small ...
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Epidermal Cell Analysis by RT-PCR
2004Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction ...
Robert Man-Kit Cheung +2 more
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1994
The application of the polymerase chain reaction technique (PCR) to the study of gene expression, variously referred in the literature to as cDNA-PCR reverse transcription-PCR (RT-PCR) (Chelly et al., 1988; Rappolee et al., 1988a) and sometimes as Patty (PCR aided transcript titration assay, Becker-Andre and Hahlbrock, 1989), represents a dramatic ...
Jamel Chelly +3 more
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The application of the polymerase chain reaction technique (PCR) to the study of gene expression, variously referred in the literature to as cDNA-PCR reverse transcription-PCR (RT-PCR) (Chelly et al., 1988; Rappolee et al., 1988a) and sometimes as Patty (PCR aided transcript titration assay, Becker-Andre and Hahlbrock, 1989), represents a dramatic ...
Jamel Chelly +3 more
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1994
Quantitative analysis of RNA is an important aspect of gene expression studies and central to the understanding of the mechanisms that regulate gene activity. Northern gels, dot or slot blots, are currently used for analyzing RNA levels, however, Northern analysis requires large quantities of RNA and is not very sensitive for quantitative use in ...
Jamel Chelly, Axel Kahn
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Quantitative analysis of RNA is an important aspect of gene expression studies and central to the understanding of the mechanisms that regulate gene activity. Northern gels, dot or slot blots, are currently used for analyzing RNA levels, however, Northern analysis requires large quantities of RNA and is not very sensitive for quantitative use in ...
Jamel Chelly, Axel Kahn
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Immunobead RT-PCR versus regular RT-PCR amplification of CEA mRNA in peripheral blood
Journal of Cancer Research and Clinical Oncology, 2001The reverse transcription polymerase chain reaction (RT-PCR) amplification of tumor-specific mRNA has been used for the detection of cancer cells in peripheral blood. More recently, an immunomagnetic isolation and reverse transcription polymerase chain reaction (immunobead RT-PCR) was developed which has reportedly significant advantages over the ...
Hong-Keun Chung +10 more
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Automated high throughput RT‐PCR
Laboratory Robotics and Automation, 1996AMGEN's genome project is designed to discover and assess genes that may be of therapeutic value. As an early screen, novel genes of potential importance are investigated by analysis of their in vivo expression pattern using reverse transcriptase-polymerase chain reaction (RT-PCR).
Arlen R. Thomason +4 more
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2017
RT-PCR is that technology by which RNA molecules are converted into their complementary DNA (cDNA) sequences by reverse transcriptase, followed by the amplification of the newly synthesized cDNA by standard PCR procedures. This approach to study gene expression is universally known as RT-PCR, because of the role of reverse transcriptase (RT) in the ...
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RT-PCR is that technology by which RNA molecules are converted into their complementary DNA (cDNA) sequences by reverse transcriptase, followed by the amplification of the newly synthesized cDNA by standard PCR procedures. This approach to study gene expression is universally known as RT-PCR, because of the role of reverse transcriptase (RT) in the ...
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