Results 231 to 240 of about 1,155,913 (246)
Some of the next articles are maybe not open access.
2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA +3 more
openaire +3 more sources
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA +3 more
openaire +3 more sources
Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genotyping by Fragment RT-PCR
2021Polymerase chain reaction (PCR) is a widely used technique in the diagnosis of viral infections due to its low cost, high sensitivity, and specificity. Although the more advanced variations of PCR, such as real-time PCR and digital PCR are now available to researchers, conventional PCR is still used in many research studies.
openaire +2 more sources
Quantitative RT-PCR for MicroRNAs in Biofluids
2017MicroRNAs in biofluids hold great promise as minimally invasive diagnostic biomarkers for a wide range of diseases and biological processes. One of the most sensitive technologies for detection and measuring expression levels of microRNA is quantitative RT-PCR.
Michael Thorsen +2 more
openaire +3 more sources
1995
Many PCR protocols for determination of specific mRNAs are based on the synthesis of cDNA in one sample tube and following amplification of an aliquot of RT sample in another reaction tube. This way is preferable when estimating different mRNAs in one RNA/cDNA sample (chapter 2.3). In this case oligo (dT) priming is recommended to obtain cDNA templates
openaire +2 more sources
Many PCR protocols for determination of specific mRNAs are based on the synthesis of cDNA in one sample tube and following amplification of an aliquot of RT sample in another reaction tube. This way is preferable when estimating different mRNAs in one RNA/cDNA sample (chapter 2.3). In this case oligo (dT) priming is recommended to obtain cDNA templates
openaire +2 more sources
Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
openaire +2 more sources
RT-PCR OF FATTY ACID ELONGASES [PDF]
Biochemical Society Transactions, 1997 P. V. Maynard, Allan P. Kells
openaire +2 more sources