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Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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Epidermal Cell Analysis by RT-PCR
2004Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction ...
Tammy-Claire, Troy +2 more
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1995
Many PCR protocols for determination of specific mRNAs are based on the synthesis of cDNA in one sample tube and following amplification of an aliquot of RT sample in another reaction tube. This way is preferable when estimating different mRNAs in one RNA/cDNA sample (chapter 2.3). In this case oligo (dT) priming is recommended to obtain cDNA templates
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Many PCR protocols for determination of specific mRNAs are based on the synthesis of cDNA in one sample tube and following amplification of an aliquot of RT sample in another reaction tube. This way is preferable when estimating different mRNAs in one RNA/cDNA sample (chapter 2.3). In this case oligo (dT) priming is recommended to obtain cDNA templates
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