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2002
Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small ...
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Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small ...
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Volumetric, Microfluidic Plasmonic RT‐PCR
Small MethodsAbstractDecentralized molecular detection of pathogens remains an important goal for public health. Although polymerase chain reaction (PCR) remains the gold‐standard molecular detection method, thermocycling using Peltier heaters presents challenges in decentralized settings. Recent work has demonstrated plasmonic PCR, where nanomaterials on a surface
Harshit Harpaldas Chellani +15 more
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Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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Epidermal Cell Analysis by RT-PCR
2004Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction ...
Tammy-Claire, Troy +2 more
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1995
Many PCR protocols for determination of specific mRNAs are based on the synthesis of cDNA in one sample tube and following amplification of an aliquot of RT sample in another reaction tube. This way is preferable when estimating different mRNAs in one RNA/cDNA sample (chapter 2.3). In this case oligo (dT) priming is recommended to obtain cDNA templates
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Many PCR protocols for determination of specific mRNAs are based on the synthesis of cDNA in one sample tube and following amplification of an aliquot of RT sample in another reaction tube. This way is preferable when estimating different mRNAs in one RNA/cDNA sample (chapter 2.3). In this case oligo (dT) priming is recommended to obtain cDNA templates
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