Label‐Free FET Based Biosensor for Detection of Engineered CAR T‐Cells at Single Cell Resolution
A silicon nanowire field‐effect transistor (SiNW FET) biosensor that allows for label‐free, real‐time detection and quantification of engineered Chimeric Antigen Receptor (CAR) T‐cells at single‐cell resolution is presented. By mimicking the CAR‐specific antigen interface, the platform provides highly selective analysis of individual cell surface ...
Trang Anh Nguyen‐Le+5 more
wiley +1 more source
Computational identification and experimental validation of novel <i>Saccharum officinarum</i> microRNAs along with their targets through RT-PCR approach. [PDF]
Baqi A+11 more
europepmc +1 more source
Analysis of Igf2 and Igf2r Genes Expression by RT-PCR in Bovine Fetuses.
Yosuke Kawase+5 more
openalex +2 more sources
Digital Bioassays on the Slipchip Microfluidic Devices
This review summarizes recent advances in SlipChip‐based digital bioassays, highlighting their simple “load and slip” operation for fluid partitioning which eliminate the need for sophisticated fluidic systems. Applications include digital nucleic acid analysis, digital protein analysis and single‐cell analysis, demonstrating the platform‘s potential ...
Qingqing Luo+5 more
wiley +1 more source
Tau protein plays a critical role in the pathology of Alzheimer's disease, utilizing extracellular vesicles (EVs) as a mechanism for intercellular transmission. This study develops a highly sensitive sandwich assay biosensor for detecting and quantifying tau‐associated EVs using surface‐enhanced Raman spectroscopy (SERS).
Saqer Al Abdullah+6 more
wiley +1 more source
Gut microbiome and clinical and lifestyle host factors associated with recurrent positive RT-PCR for SARS-CoV-2. [PDF]
Jiménez-Arroyo C+8 more
europepmc +1 more source
Cloning of Human Picobirnavirus Genomic Segments and Development of an RT-PCR Detection Assay
B I Rosen+3 more
openalex +1 more source
Cancer cell‐intrinsic SMYD3 orchestrates an immunosuppressive microenvironment and impairs the response to PD‐1 blockade by reprogramming immune cells landscape in the tumor microenvironment of clear cell renal cell carcinoma (ccRCC). The immunomodulatory function of SMYD3 is mediated via the SREBP1/CD47 pathway.
Zhengfang Liu+14 more
wiley +1 more source
Establishment of a duplex TaqMan-based real time RT-PCR assay for simultaneous detection of BRSV and BVDV. [PDF]
Hao F+6 more
europepmc +1 more source
Loss of NR2F6 Protects from Salmonella Typhimurium Infection
Loss of nuclear receptor NR2F6 reduces tissue‐resident macrophage populations. Nr2f6‐deficient mice are protected from weight loss and bacterial load during infection with Salmonella Typhimurium. Pro‐inflammatory cytokines and iron levels are altered in infected Nr2f6‐deficient mice.
Johannes Woelk+8 more
wiley +1 more source