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Molecules and Cells: RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature ...
Dajeong Bong +2 more
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Tutorial: Guidelines for Single-Cell RT-qPCR [PDF]
Cells, 2021 Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha +2 more
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Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells [PDF]
Biological Procedures Online, 2010 Background Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs
Chen Gang +3 more
doaj +2 more sources
A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing [PDF]
BiosensorsPoint-of-care testing (POCT) has emerged as a vital diagnostic approach in emergency medicine, primary care, and resource-limited environments because of its convenience, affordability, and capacity to provide immediate results.
Baihui Zhang +10 more
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Intratumoral heterogeneity of microRNA expression in rectal cancer [PDF]
, 2016 Introduction: An increasing number of studies have investigated microRNAs (miRNAs) as potential markers of diagnosis, treatment and prognosis.
A Esquela-Kerscher +34 more
core +17 more sources
Selection of stable reference genes for RT–qPCR in Salmo trutta
Aquaculture Reports, 2022 To identify suitable reference genes for Salmo trutta, real-time fluorescent quantitative PCR (RT–qPCR) technology was used to determine the expression of eight candidate genes in 11 tissues (heart, liver, spleen, head kidney, muscle, brain, gills, ovary,
Shuaijie Sun +10 more
doaj +1 more source
A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]
PLoS ONE, 2016 Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier +5 more
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In Situ Capture RT-qPCR Method for Detection of Human Norovirus in Food and Environmental Samples
Proceedings, 2020 Human noroviruses (HuNoVs) are the major cause of non-bacterial acute gastroenteritis worldwide. RT-qPCR is a widely used method to detect HuNoVs. However, the method is unable to extract a virus from environmental samples and to discriminate between ...
Peng Tian
doaj +1 more source
Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection
International Journal of Infectious Diseases, 2021 Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study
Masato Inaba +7 more
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Pathogens, 2021 Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the ...
Laurence Thirion +7 more
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