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Brief guide to RT-qPCR. [PDF]

Mol Cells
RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature, sensitivity, and specificity.
Bong D, Sohn J, Lee SV.
europepmc   +4 more sources

Tutorial: Guidelines for Single-Cell RT-qPCR [PDF]

Cells, 2021
Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha, Mikael Kubista, Lukas Valihrach   +2 more
doaj   +3 more sources

A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing [PDF]

Biosensors
Point-of-care testing (POCT) has emerged as a vital diagnostic approach in emergency medicine, primary care, and resource-limited environments because of its convenience, affordability, and capacity to provide immediate results.
Baihui Zhang, Xiao Li, Mengjie Huang, Maojie Jiang, Leilei Du, Peng Yin, Xuan Fang, Xiangyu Jiang, Feihu Qi, Yanna Lin, Fuqiang Ma   +10 more
doaj   +2 more sources

Intratumoral heterogeneity of microRNA expression in rectal cancer [PDF]

, 2016
Introduction: An increasing number of studies have investigated microRNAs (miRNAs) as potential markers of diagnosis, treatment and prognosis.
A Esquela-Kerscher, AHM Eriksen, Anders Jakobsen, Ane Lindegaard Appelt, Anne Haahr Mellergaard Eriksen, Boye Schnack Nielsen, BS Nielsen, CJ Wang, D Hanahan, D Hanahan, EA Kheirelseid, EM Laurila, Flemming Brandt Sørensen, GH Heppner, HJ Peltier, J Peveling-Oberhag, JG Kuremsky, KH Chang, KN Knudsen, M Agostini, M Raychaudhuri, M Svoboda, O Slaby, O Slaby, PA Davoren, Rajvir Dahiya, Rikke Fredslund Andersen, S Jorgensen, Scarpati G Della Vittoria, SH Lim, T Li, Torben Frøstrup Hansen, U Drebber, W Filipowicz, Z Wotschofsky   +34 more
core   +14 more sources

Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater

ACS ES&T water, 2022
We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low-prevalence COVID-19 locations in Queensland, Australia, using CDC N1 and CDC N2 ...
W. Ahmed, W. Smith, S. Metcalfe, Greg Jackson, Phil M. Choi, Mary Morrison, Daniel Field, P. Gyawali, A. Bivins, K. Bibby, S. Simpson   +10 more
semanticscholar   +1 more source

A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]

PLoS ONE, 2016
Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier, Audrey Fraisse, Sandra Martin-Latil, Sabine Delannoy, Patrick Fach, Sylvie Perelle   +5 more
doaj   +1 more source

Selection of stable reference genes for RT–qPCR in Salmo trutta

Aquaculture Reports, 2022
To identify suitable reference genes for Salmo trutta, real-time fluorescent quantitative PCR (RT–qPCR) technology was used to determine the expression of eight candidate genes in 11 tissues (heart, liver, spleen, head kidney, muscle, brain, gills, ovary,
Shuaijie Sun, Zhitong Wang, Dongdong Yuan, Mengke Ni, Huifen Xu, Wanliang Wang, Chi Zhang, Meiqun Chen, Lamu ZhaXi, Jianshe Zhou, Ming Li   +10 more
doaj   +1 more source

Multiplex RT-qPCR Application in Early Detection of Bovine Respiratory Disease in Healthy Calves

Viruses, 2023
Bovine respiratory diseases (BRD) are associated with various predisposing factors, such as physical and physiological stress factors, and bacterial and viral pathogens. These stressors and viruses suppress immune defenses, leading to bacterial growth in
Yusuke Goto, K. Fukunari, Tohru Suzuki
semanticscholar   +1 more source

The Inhibition and Variability of Two Different RT-qPCR Assays Used for Quantifying SARS-CoV-2 RNA in Wastewater

Food and Environmnetal Virology, 2023
Faecal shedding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its subsequent detection in wastewater turned the spotlight onto wastewater-based epidemiology (WBE) for monitoring the coronavirus-disease 2019 (COVID-19) pandemic.
G. Scott, N. Evens, J. Porter, David I. Walker   +3 more
semanticscholar   +1 more source

COVID-19 Diagnosis: A Comprehensive Review of the RT-qPCR Method for Detection of SARS-CoV-2

Diagnostics, 2022
The world is grappling with the coronavirus disease 2019 (COVID-19) pandemic, the causative agent of which is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Debashis Dutta, Sarah Naiyer, Sabanaz Mansuri, Neeraj Soni, Vandana Singh, K. H. Bhat, Nishant Singh, G. Arora, M. Mansuri   +8 more
semanticscholar   +1 more source