Failure to remove bluetongue serotype 8 virus (BTV-8) from in vitro produced and in vivo derived bovine embryos and subsequent transmission of BTV-8 to recipient cows after embryo transfer [PDF]
, 2019 The behavior of BTV-8 in cattle is different from most other serotypes not only with regards to clinical signs but certainly with respect to virus transmission (transplacental, contact). Therefore, the possibility of virus transmission by means of embryo
De Clercq, Kris +6 more
core +1 more source
Detection of Chikungunya Virus RNA in Oral Fluid and Urine: An Alternative Approach to Diagnosis?
VirusesTo evaluate whether oral fluids (OF) and urine can serve as alternative, non-invasive samples to diagnose chikungunya virus (CHIKV) infection via RT-qPCR, we employed the same RNA extraction and RT-qPCR protocols on paired serum, OF and urine samples ...
Leile Camila Jacob-Nascimento +8 more
doaj +1 more source
STAT3, a hub protein of cellular signaling pathways, is triggered by β-hexaclorocyclohexane [PDF]
, 2018 Background: Organochlorine pesticides (OCPs) are widely distributed in the environment and their toxicity is mostly associated with the molecular mechanisms of endocrine disruption.
Altieri, F +7 more
core +2 more sources
Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages [PDF]
, 2018 Background: As crucial regulators of the immune response against pathogens, macrophages have been extensively shown also to be important players in several diseases, including cancer. Specifically, breast cancer macrophages tightly control the angiogenic
Blandino, Giovanni +14 more
core +3 more sources
Journal of Rawalpindi Medical CollegeObjective: The objective of this study was to compare the diagnostic accuracy of RT-qPCR in endometrial cancer using novel biomarkers in clinical specimens, keeping immunohistochemical analysis as the gold standard.
Sampana Fatima +4 more
doaj +1 more source
Circular RNAs in Clear Cell Renal Cell Carcinoma: Their Microarray-Based Identification, Analytical Validation, and Potential Use in a Clinico-Genomic Model to Improve Prognostic Accuracy [PDF]
, 2019 Circular RNAs (circRNAs) may act as novel cancer biomarkers. However, a genome-wide evaluation of circRNAs in clear cell renal cell carcinoma (ccRCC) has yet to be conducted. Therefore, the objective of this study was to identify and validate circRNAs in
Erbersdobler, Andreas +9 more
core +2 more sources
Rabbit calicivirus capsid Taqman RT-qPCR v1 [PDF]
, 2019 To improve the sensitivity and throughput of molecular testing for rabbit caliciviruses we have developed a multiplex TaqMan RT-qPCR assay that detects the three different pathogenic rabbit calicivirus capsid genotypes currently circulating in Australia.
Robyn Hall +4 more
openaire +1 more source
Microrna response of primary human macrophages to Arcobacter Butzleri infection [PDF]
, 2016 The role of microRNAs (miRNAs) in infectious diseases is becoming more and more apparent, and the use of miRNAs as a diagnostic tool and their therapeutic application has become the major focus of investigation.
Alter, Thomas +9 more
core +3 more sources
Rat ACTB one-step RT-qPCR v1 [PDF]
, 2018 RNA from matched FFPE, PFPE and cryo preserved rat tissues, stored for up to nine years at 22°C, 4°C, -20°C or -80°C, examined for integrity and usability in quantitative RT-PCR
Daniel Groelz, Nadine Dettmann
openaire +1 more source
Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions [PDF]
, 2019 Fungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population.
Coucke, Paul +3 more
core +1 more source