Results 111 to 120 of about 1,081,218 (337)
A convenient, optimized pipeline for isolation, fluorescence microscopy and molecular analysis of live single cells [PDF]
, 2014 BACKGROUND: Heterogeneity within cell populations is relevant to the onset and progression of disease, as well as development and maintenance of homeostasis.
Colleen P Ziegler +4 more
core +2 more sources
A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
Scientific Reports, 2020 The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step in RT-qPCR, often achieved using commercial kits.
A. Wozniak +15 more
semanticscholar +1 more source
Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics
Archives of Virology, 2021 Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the “gold standard” in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we
A. Domnich +6 more
semanticscholar +1 more source
, 2018 RNA from matched FFPE, PFPE and cryo preserved human tissues, stored for up to seven years at 22°C and 4°C, examined for integrity and usability in quantitative RT-PCR
openaire +1 more source
RT-qPCR Detection of Senescence-Associated Circular RNAs [PDF]
, 2016 Primary cells that reach the end of their replicative potential, encounter sublethal stress, or experience the activation of certain oncogenes cease proliferation and enter a state of long-term growth arrest named senescence. The senescent process has been implicated in a variety of age-related diseases and also in the pathogenesis of cancer ...
Amaresh C, Panda +2 more
openaire +2 more sources
Molecular Oncology, EarlyView.Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié +16 more
wiley +1 more source
Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR.
PLoS ONEIntroductionDengue fever is a growing global concern with an estimated 100-400 million infections every year and rising mortality over the past decade. In 2017, 40,000 deaths were attributed to dengue. Real-time reverse transcription PCR (RT-qPCR) is the
Vilayouth Phimolsannousith +5 more
doaj +1 more source
Frontiers in MicrobiologyBackgroundThere are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-
Hua-Wei Yi +15 more
doaj +1 more source
Canine distemper virus detection by different methods of One-Step RT-qPCR
Ciência Rural, 2016 : Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical ...
Claudia de Camargo Tozato +3 more
doaj +1 more source
Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice [PDF]
, 2014 Clinical and epidemiologic data suggest that obesity is associated with more aggressive forms of prostate cancer, poor prognosis, and increased mortality.
Cotignola, Javier Hernan +11 more
core +1 more source