Results 11 to 20 of about 491,020 (188)

Rapid and accurate quantification of isomiRs by RT-qPCR

Scientific Reports, 2022
Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we
Sandra Franco, Raquel Pluvinet, Jose Francisco Sanchez-Herrero, Lauro Sumoy, Miguel Angel Martinez   +4 more
doaj   +5 more sources

Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses

Engineering, 2020
Human noroviruses (HuNoVs) are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide. As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus, detection is mainly dependent on molecular ...
Danlei Liu, Zilei Zhang, Qingping Wu, Peng Tian, Haoran Geng, Ting Xu, Dapeng Wang   +6 more
doaj   +1 more source

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA

Scientific Reports, 2023
Rift valley fever (RVF) is an important zoonotic disease caused by the Rift valley fever virus (RVFV) which can affect ruminants and humans. In this study, a comparison was done of the reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Changwoo Park, Dongju Park, Zohaib Ul Hassan, Sang Ho Choi, Seil Kim   +4 more
doaj   +1 more source

SARS-CoV-2 nucleic acid shedding is variable for every person

Revista da Sociedade Brasileira de Medicina Tropical, 2021
INTRODUCTION: Considering the persistent positivity on RT-qPCR tests, the results of SARS-CoV-2 were monitored to evaluate the viral RNA shedding period.
Daiana de Lima-Morales, Priscila Lamb Wink, Fabiana Caroline Zempulski Volpato, Joíza Lins Camargo, Fernanda de-Paris, Afonso Luís Barth   +5 more
doaj   +1 more source

Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]

, 2016
Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude, De Smet, Els, Mestdagh, Pieter, Vanderheyden, Katrien, Vandesompele, Jo, Zeka, Fjoralba   +5 more
core   +2 more sources

Molekularbiologische Diagnostik von Pflanzenkrankkeiten

Julius-Kühn-Archiv, 2014
In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine, Gottsberger, Richard A., Leichtfried, Thomas, Reisenzein, Helga   +3 more
doaj   +1 more source

RT-qPCR half-reaction optimization for the detection of SARS-CoV-2

Revista da Sociedade Brasileira de Medicina Tropical, 2021
INTRODUCTION: The main laboratory test for the diagnosis of coronavirus disease 2019 (COVID-19) is the reverse transcription real-time polymerase chain reaction (RT-qPCR). However, RT-qPCR is expensive because of the number of tests required. This study
Priscila Lamb Wink, Fabiana Volpato, Daiana de Lima-Morales, Rodrigo Minuto Paiva, Julia Biz Willig, Hugo Bock, Fernanda de Paris, Afonso Luís Barth   +7 more
doaj   +1 more source

Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]

, 2012
Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Van Peer, Gert, Mestdagh, Pieter, Vandesompele, Jo   +2 more
core   +1 more source

High-throughput microfluidic single-cell RT-qPCR [PDF]

Proceedings of the National Academy of Sciences, 2011
A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run.
Adam K, White, Michael, VanInsberghe, Oleh I, Petriv, Mani, Hamidi, Darek, Sikorski, Marco A, Marra, James, Piret, Samuel, Aparicio, Carl L, Hansen   +8 more
openaire   +2 more sources

Analytic lymph node number establishes staging accuracy by occult tumor burden in colorectal cancer. [PDF]

, 2012
BACKGROUND AND OBJECTIVES: Recurrence in lymph node-negative (pN0) colorectal cancer suggests the presence of undetected occult metastases. Occult tumor burden in nodes estimated by GUCY2C RT-qPCR predicts risk of disease recurrence.
Barkun, Alan, Hyslop, Terry, Schulz, Stephanie, Waldman, Scott A., Weinberg, David S.   +4 more
core   +2 more sources