Results 11 to 20 of about 1,081,218 (337)
RT-qPCR Testing of SARS-CoV-2: A Primer [PDF]
International Journal of Molecular Sciences, 2020 Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely ...
S. Bustin, T. Nolan
semanticscholar +5 more sources
Rapid and accurate quantification of isomiRs by RT-qPCR
Scientific Reports, 2022 Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we
Sandra Franco +4 more
doaj +5 more sources
Viruses, 2022 Avian influenza virus (AIV) variants emerge frequently, which challenges rapid diagnosis. Appropriate diagnosis reaching the sub- and pathotype level is the basis of combatting notifiable AIV infections.
Kareem E Hassan +7 more
semanticscholar +1 more source
In Situ Capture RT-qPCR Method for Detection of Human Norovirus in Food and Environmental Samples
Proceedings, 2020 Human noroviruses (HuNoVs) are the major cause of non-bacterial acute gastroenteritis worldwide. RT-qPCR is a widely used method to detect HuNoVs. However, the method is unable to extract a virus from environmental samples and to discriminate between ...
Peng Tian
doaj +1 more source
Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection
International Journal of Infectious Diseases, 2021 Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study
Masato Inaba +7 more
doaj +1 more source
Evaluation of COVID-19 RT-qPCR test in multi-sample pools
medRxiv, 2020 The recent emergence of SARS-CoV-2 lead to a current pandemic of unprecedented levels. Though diagnostic tests are fundamental to the ability to detect and respond, many health systems are already experiencing shortages of reagents associated with this ...
I. Yelin +15 more
semanticscholar +1 more source
BMC Bioinformatics, 2021 The analyses of amplification and melting curves have been shown to provide valuable information on the quality of the individual reactions in quantitative PCR (qPCR) experiments and to result in more reliable and reproducible quantitative results.
A. Untergasser +3 more
semanticscholar +1 more source
Quantitative SARS-CoV-2 Alpha Variant B.1.1.7 Tracking in Wastewater by Allele-Specific RT-qPCR
Environmental Science and Technology Letters, 2021 The critical need for surveillance of SARS-CoV-2 variants of concern has prompted the development of methods that can track variants in wastewater. Here, we develop and present an open-source method based on allele-specific RT-qPCR (AS RT-qPCR) that ...
W. Lee +21 more
semanticscholar +1 more source
Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses
Engineering, 2020 Human noroviruses (HuNoVs) are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide. As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus, detection is mainly dependent on molecular ...
Danlei Liu +6 more
doaj +1 more source
Pathogens, 2021 Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the ...
Laurence Thirion +7 more
doaj +1 more source