Results 21 to 30 of about 491,020 (188)
Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]
, 2013 Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra +6 more
core +2 more sources
Impact of Capsid and Genomic Integrity Tests on Norovirus Extraction Recovery Rates
Foods, 2023 Human norovirus (HuNoV) is the leading pathogen responsible for food-borne illnesses. However, both infectious and non-infectious HuNoV can be detected by RT-qPCR.
Philippe Raymond +2 more
doaj +1 more source
Angiopoietins differentially influence in vitro angiogenesis by endothelial cells of different origin [PDF]
, 2011 Angiopoietins are important growth factors for vascular development and quiescence. They are promising targets for pro-or anti-angiogenic therapies in diverse pathologies, but the mechanisms of the ANGPT/TIE2 system are complex and not well understood ...
Bahramsoltani, Mahtab +4 more
core +2 more sources
BMC Infectious Diseases, 2020 Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya +4 more
doaj +1 more source
Scientific Reports, 2022 Saliva has been demonstrated as feasible alternative to naso-oropharyngeal swab (NOS) for SARS-CoV-2 detection through reverse transcription quantitative/real-time polymerase chain reaction (RT-qPCR).
Michael L. Tee +9 more
doaj +1 more source
Brazilian Journal of Infectious Diseases, 2023 Introdução/objetivos: As gastroenterites agudas (GEA) são doenças que se constituem como a segunda maior causa de morte em crianças de todo mundo, especialmente em crianças menores de cinco anos, ocorrendo principalmente em países de baixa e média renda.
Ana Luiza da Mota Raminho +5 more
doaj +1 more source
Stem‐Loop RT‐qPCR for miRNAs [PDF]
Current Protocols in Molecular Biology, 2011 AbstractThis unit presents a specific and sensitive quantitative reverse‐transcription PCR (RT‐qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24 nucleotides (nt) in length. Standard and quantitative PCR methods require a template that is at least two times the length of either of the specific ...
openaire +2 more sources
COVID-19 diagnosis by RT-qPCR in alternative specimens
Memorias do Instituto Oswaldo Cruz, 2021 BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent ...
Cássia Cristina Alves Gonçalves +22 more
doaj +1 more source
Cross platform standardisation and normalisation experimental pipeline for use in the biodiscovery of dysregulated human circulating miRNAs [PDF]
, 2015 Introduction. Micro RNAs (miRNAs) are a class of highly conserved small non-coding RNAs that play an important part in the post-transcriptional regulation of gene expression.
Downing, Tim, Kelly, Helena
core +1 more source
Large-Scale RT-qPCR Diagnostics for Seed Potato Certification [PDF]
Potato Research, 2021 AbstractEvery year, Agroscope examines nearly 300,000 tubers for the presence of viruses, as regulated for the certification of seed potatoes intended for Swiss growers. Since 2016, this examination has been performed via RT-qPCR on dormant tubers directly after harvest.
Olivier Schumpp +7 more
openaire +2 more sources