Results 21 to 30 of about 1,081,218 (337)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA

Scientific Reports, 2023
Rift valley fever (RVF) is an important zoonotic disease caused by the Rift valley fever virus (RVFV) which can affect ruminants and humans. In this study, a comparison was done of the reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Changwoo Park, Dongju Park, Zohaib Ul Hassan, Sang Ho Choi, Seil Kim   +4 more
doaj   +1 more source

Obtaining Reliable RT-qPCR Results in Molecular Diagnostics—MIQE Goals and Pitfalls for Transcriptional Biomarker Discovery

Life, 2022
In this review, we discuss the development pipeline for transcriptional biomarkers in molecular diagnostics and stress the importance of a reliable gene transcript quantification strategy.
C. Grätz, Maria L U Bui, Granit Thaqi, B. Kirchner, Robert P. Loewe, M. Pfaffl   +5 more
semanticscholar   +1 more source

SARS-CoV-2 nucleic acid shedding is variable for every person

Revista da Sociedade Brasileira de Medicina Tropical, 2021
INTRODUCTION: Considering the persistent positivity on RT-qPCR tests, the results of SARS-CoV-2 were monitored to evaluate the viral RNA shedding period.
Daiana de Lima-Morales, Priscila Lamb Wink, Fabiana Caroline Zempulski Volpato, Joíza Lins Camargo, Fernanda de-Paris, Afonso Luís Barth   +5 more
doaj   +1 more source

S-variant SARS-CoV-2 lineage B1.1.7 is associated with significantly higher viral loads in samples tested by ThermoFisher TaqPath RT-qPCR

Journal of Infectious Diseases, 2021
A SARS-CoV-2 variant B1.1.7 containing a mutation Δ69/70 has spread rapidly in the UK and shows an identifiable profile in ThermoFisher TaqPath RTqPCR (S-gene target failure; SGTF). We analysed recent test data for trends and significance.
M. Kidd, A. Richter, A. Best, Nicola Cumley, J. Mirza, B. Percival, M. Mayhew, O. Megram, F. Ashford, T. White, E. Moles-Garcia, L. Crawford, A. Bosworth, S. Atabani, T. Plant, A. McNally   +15 more
semanticscholar   +1 more source

DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP

bioRxiv, 2020
The ongoing SARS-CoV-2 pandemic has caused an unprecedented need for rapid diagnostic screening [1]. The primary assay employed is a reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay that requires the use of an RNA extraction ...
E. Bruce, S. Tighe, Jessica J Hoffman, Pheobe Laaguiby, D. Gerrard, S. Diehl, D. Leonard, C. Huston, B. Kirkpatrick, Jessica W. Crothers, J. Dragon, J. Botten   +11 more
semanticscholar   +2 more sources

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19

Journal of Clinical Microbiology, 2020
The clinical performances of six molecular diagnostic tests and a rapid antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were clinically evaluated for the diagnosis of coronavirus disease 2019 (COVID-19) in self-collected ...
M. Nagura-Ikeda, K. Imai, S. Tabata, K. Miyoshi, Nami Murahara, Tsukasa Mizuno, M. Horiuchi, Kento Kato, Yoshitaka Imoto, Makiko Iwata, S. Mimura, Toshimitsu Ito, Kaku Tamura, Y. Kato   +13 more
semanticscholar   +1 more source

Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children

Nature Communications, 2022
Systematic SARS-CoV-2 testing is a valuable tool for infection control and surveillance. However, broad application of high sensitive RT-qPCR testing in children is often hampered due to unpleasant sample collection, limited RT-qPCR capacities and high ...
F. Dewald, I. Suárez, R. Johnen, J. Grossbach, Roberto Morán-Tovar, G. Steger, Alexander Joachim, Gibran Horemheb Rubio, Mira Fries, Florian Behr, J. Kley, A. Lingnau, A. Kretschmer, Carina Gude, Guadelupe Baeza-Flores, David Laveaga del Valle, Alberto Roblero-Hernández, Jesús M. Magaña-Cerino, Adriana Torres Hernandez, J. Ruiz-Quiñones, Konstantin Schega, Viktoria Linne, Lena Junker, Marie Wunsch, E. Heger, E. Knops, V. Di Cristanziano, M. Meyer, C. Hünseler, L. Weber, J. Lüers, G. Quade, H. Wisplinghoff, C. Tiemann, R. Zotz, H. Jomaa, A. Pranada, I. Herzum, Paul A. Cullen, F. Schmitz, Paul Philipsen, G. Kirchner, C. Knabbe, M. Hellmich, M. Buess, A. Wolff, A. Kossow, J. Niessen, Sebastian Jeworutzki, Jörg-Peter Schräpler, M. Lässig, J. Dötsch, G. Fätkenheuer, R. Kaiser, A. Beyer, J. Rybniker, F. Klein   +56 more
semanticscholar   +1 more source

Molekularbiologische Diagnostik von Pflanzenkrankkeiten

Julius-Kühn-Archiv, 2014
In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine, Gottsberger, Richard A., Leichtfried, Thomas, Reisenzein, Helga   +3 more
doaj   +1 more source

Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]

, 2016
Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude, De Smet, Els, Mestdagh, Pieter, Vanderheyden, Katrien, Vandesompele, Jo, Zeka, Fjoralba   +5 more
core   +2 more sources

Direct comparison of Xpert Xpress, FilmArray Respiratory Panel, Lumipulse antigen test, and RT-qPCR in 165 nasopharyngeal swabs

BMC Infectious Diseases, 2022
Background The nucleic acid amplification test (NAAT) and antigen test are approved diagnostic tests for COVID-19. In this study, we aimed to investigate the assay performance of two NAATs (Xpert Xpress SARS-CoV-2 and FilmArray Respiratory Panel) and a ...
Y. Hirotsu, M. Maejima, M. Shibusawa, Yume Natori, Y. Nagakubo, K. Hosaka, H. Sueki, K. Amemiya, M. Hayakawa, H. Mochizuki, T. Tsutsui, Y. Kakizaki, Y. Miyashita, Masao Omata   +13 more
semanticscholar   +1 more source