Results 31 to 40 of about 1,081,218 (337)

RT-qPCR half-reaction optimization for the detection of SARS-CoV-2

Revista da Sociedade Brasileira de Medicina Tropical, 2021
INTRODUCTION: The main laboratory test for the diagnosis of coronavirus disease 2019 (COVID-19) is the reverse transcription real-time polymerase chain reaction (RT-qPCR). However, RT-qPCR is expensive because of the number of tests required. This study
Priscila Lamb Wink, Fabiana Volpato, Daiana de Lima-Morales, Rodrigo Minuto Paiva, Julia Biz Willig, Hugo Bock, Fernanda de Paris, Afonso Luís Barth   +7 more
doaj   +1 more source

Comparison of Electronegative Filtration to Magnetic Bead-Based Concentration and V2G-qPCR to RT-qPCR for Quantifying Viral SARS-CoV-2 RNA from Wastewater.

ACS ES&T water, 2022
Methods of wastewater concentration (electronegative filtration (ENF) versus magnetic bead-based concentration (MBC)) were compared for the analysis of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), beta-2 microglobulin, and human ...
K. Babler, A. Amirali, M. Sharkey, Sion L. Williams, M. Boone, Gabriella A. Cosculluela, B. Currall, G. Grills, J. Laine, C. Mason, B. Reding, S. Schürer, M. Stevenson, D. Vidović, H. Solo-Gabriele   +14 more
semanticscholar   +1 more source

Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]

, 2012
Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Mestdagh, Pieter, Van Peer, Gert, Vandesompele, Jo   +2 more
core   +2 more sources

In silico evaluation of the impact of Omicron variant of concern sublineage BA.4 and BA.5 on the sensitivity of RT‐qPCR assays for SARS‐CoV‐2 detection using whole genome sequencing

Journal of Medical Virology, 2022
Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) variant of concern (VoC) Omicron (B.1.1.529) has rapidly spread around the world, presenting a new threat to global public human health. Due to the large number of mutations accumulated by SARS‐
Divya Sharma, K. Notarte, R. A. Fernandez, G. Lippi, M. Michael Gromiha, B. Henry   +5 more
semanticscholar   +1 more source

High-throughput microfluidic single-cell RT-qPCR [PDF]

Proceedings of the National Academy of Sciences, 2011
A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run.
Adam K, White, Michael, VanInsberghe, Oleh I, Petriv, Mani, Hamidi, Darek, Sikorski, Marco A, Marra, James, Piret, Samuel, Aparicio, Carl L, Hansen   +8 more
openaire   +2 more sources

A novel and universal method for microRNA RT-qPCR data normalization [PDF]

, 2009
Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR
De Weer, An, Mestdagh, Pieter, Muth, Daniel, Speleman, Franki, Van Vlierberghe, Pieter, Vandesompele, Jo, Westermann, Frank   +6 more
core   +2 more sources

True or false: what are the factors that influence COVID-19 diagnosis by RT-qPCR?

Expert Review of Molecular Diagnostics, 2022
Introduction The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease has had a catastrophic impact on the world resulting in several deaths.
Luina Benevides Lima, F. P. Mesquita, Laís Lacerda Brasil de Oliveira, Francisca Andréa da Silva Oliveira, Maria Elisabete Amaral de Moraes, P. F. Souza, R. Montenegro   +6 more
semanticscholar   +1 more source

Impact of BCR::ABL1 transcript type on RT-qPCR amplification performance and molecular response to therapy

Leukemia, 2022
Several studies have reported that chronic myeloid leukaemia (CML) patients expressing e14a2 BCR::ABL1 have a faster molecular response to therapy compared to patients expressing e13a2.
Matthew Salmon, H. White, H. Žižková, A. Gottschalk, E. Motlova, N. Cerveira, D. Colomer, Daniel Coriu, G. Franke, E. Gottardi, B. Izzo, T. Jurček, T. Lion, V. Schäfer, C. Venturi, P. Vigneri, M. Zawada, J. Zuna, Lenka Hovorkova, J. Koblihova, H. Klamová, M. Marková, D. Srbová, A. Benesova, V. Polívková, D. Žáčková, J. Mayer, I. Roeder, I. Glauche, T. Ernst, A. Hochhaus, K. Polakova, N. Cross   +32 more
semanticscholar   +1 more source

Analytic lymph node number establishes staging accuracy by occult tumor burden in colorectal cancer. [PDF]

, 2012
BACKGROUND AND OBJECTIVES: Recurrence in lymph node-negative (pN0) colorectal cancer suggests the presence of undetected occult metastases. Occult tumor burden in nodes estimated by GUCY2C RT-qPCR predicts risk of disease recurrence.
Barkun, Alan, Hyslop, Terry, Schulz, Stephanie, Waldman, Scott A., Weinberg, David S.   +4 more
core   +2 more sources

Combination of RT‐qPCR testing and clinical features for diagnosis of COVID‐19 facilitates management of SARS‐CoV‐2 outbreak

Journal of Medical Virology, 2020
In December 2019, a cluster of acute respiratory illness occurred in Wuhan, Hubei Province, China. This disease is now officially known as 2019 novel coronavirus disease (COVID-19) from WHO, novel coronavirus pneumonia This article is protected by ...
Yishan Wang, Hanyujie Kang, Xuefeng Liu, Z. Tong   +3 more
semanticscholar   +1 more source