Results 31 to 40 of about 491,020 (188)
Isoform-level gene signature improves prognostic stratification and accurately classifies glioblastoma subtypes. [PDF]
, 2014 Molecular stratification of tumors is essential for developing personalized therapies. Although patient stratification strategies have been successful; computational methods to accurately translate the gene-signature from high-throughput platform to a ...
Bi, Yingtao +5 more
core +1 more source
Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
FASEB BioAdvances, 2019 Extracellular microRNAs (miRs) have been proposed as important blood‐based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable and easily detectable in body fluids.
Maria Fauth +4 more
doaj +1 more source
International Journal of Statistics in Medical Research, 2012 We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
Perkins, James R. +2 more
openaire +2 more sources
A convenient, optimized pipeline for isolation, fluorescence microscopy and molecular analysis of live single cells [PDF]
, 2014 BACKGROUND: Heterogeneity within cell populations is relevant to the onset and progression of disease, as well as development and maintenance of homeostasis.
Colleen P Ziegler +4 more
core +2 more sources
Journal of Integrative Agriculture, 2014 Two microRNA (miRNA) quantification methods, namely, poly(A) reverse transcription (RT)-quantitative real-time polymerase chain reaction (qPCR) and stem-loop RT-qPCR, have been developed for quantifying miRNA expression. In the present study, five miRNAs,
Ran HAN +3 more
doaj +1 more source
RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts [PDF]
, 2009 Background : The quantitative polymerase chain reaction (qPCR) is a widely utilized method for gene-expression analysis. However, insufficient material often compromises large-scale gene-expression studies. The aim of this study is to evaluate an RNA pre-
Vermeulen, Joëlle +9 more
core +2 more sources
, 2021 Protocol-RT-qPCR to the paper: Viral mapping in COVID-19 deceased in the Augsburg autopsy series of the first wave: a multiorgan and multimethodological approach Full organ mapping of patients with COVID-19: an autopsy study
openaire +1 more source
Custom RT-qPCR-array for glaucoma filtering surgery prognosis. [PDF]
PLoS ONE, 2017 Excessive subconjunctival scarring is the main reason of failure of glaucoma filtration surgery. We analyzed conjunctival and systemic gene expression patterns after non penetrating deep sclerectomy (NPDS). To find expression patterns related to surgical
Iñaki Rodriguez-Agirretxe +4 more
doaj +1 more source
Biological subtyping of early breast cancer : a study comparing RT-qPCR with immunohistochemistry [PDF]
, 2016 The biological subtype of breast cancer influences the selection of systemic therapy. Distinction between luminal A and B cancers depends on consistent assessment of Ki-67, but substantial intra-observer and inter-observer variability exists when ...
Auvinen, Paivi +14 more
core +2 more sources
Prognostic value of CIP2A gene expression in adult Egyptian acute myeloid leukemia patients
Egyptian Journal of Medical Human Genetics, 2020 Background Protein phosphatase 2A (PP2A) functions as a tumor suppressor in many cancers. Cancerous inhibitor of protein phosphatase 2A (CIP2A) inhibits PP2A proteolytic degradation of c-Myc and enhances cell growth and tumor formation in several tissues.
Roxan E. Shafik +5 more
doaj +1 more source