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Frontiers in Cellular and Infection Microbiology, 2021 Background Several variants of the SARS-CoV-2 have been documented globally during the current COVID-19 pandemic. The N501Y, 69-70del, K417N, and E484K SARS-CoV-2 mutations have been documented among the most relevant due to their potential pathogenic ...
Natali Vega-Magaña +11 more
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Impact of Capsid and Genomic Integrity Tests on Norovirus Extraction Recovery Rates
Foods, 2023 Human norovirus (HuNoV) is the leading pathogen responsible for food-borne illnesses. However, both infectious and non-infectious HuNoV can be detected by RT-qPCR.
Philippe Raymond +2 more
doaj +1 more source
Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]
, 2013 Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra +6 more
core +2 more sources
Scientific Reports, 2021 Choroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for
Kim Hoa Ho, Annarita Patrizi
semanticscholar +1 more source
BMC Infectious Diseases, 2020 Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya +4 more
doaj +1 more source
Scientific Reports, 2022 Saliva has been demonstrated as feasible alternative to naso-oropharyngeal swab (NOS) for SARS-CoV-2 detection through reverse transcription quantitative/real-time polymerase chain reaction (RT-qPCR).
Michael L. Tee +9 more
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COVID-19 diagnosis by RT-qPCR in alternative specimens
Memorias do Instituto Oswaldo Cruz, 2021 BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent ...
Cássia Cristina Alves Gonçalves +22 more
doaj +1 more source
Angiopoietins differentially influence in vitro angiogenesis by endothelial cells of different origin [PDF]
, 2011 Angiopoietins are important growth factors for vascular development and quiescence. They are promising targets for pro-or anti-angiogenic therapies in diverse pathologies, but the mechanisms of the ANGPT/TIE2 system are complex and not well understood ...
Bahramsoltani, Mahtab +4 more
core +2 more sources
40 minutes RT-qPCR Assay for Screening Spike N501Y and HV69-70del Mutations
bioRxiv, 2021 A one-step reverse transcription and real-time PCR (RT-qPCR) test was developed for rapid screening (40 minutes) of the Spike N501Y and HV69-70del mutations in SARS-CoV-2 positive samples.
G. Korukluoglu +6 more
semanticscholar +1 more source
Stem‐Loop RT‐qPCR for miRNAs [PDF]
Current Protocols in Molecular Biology, 2011 AbstractThis unit presents a specific and sensitive quantitative reverse‐transcription PCR (RT‐qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24 nucleotides (nt) in length. Standard and quantitative PCR methods require a template that is at least two times the length of either of the specific ...
openaire +2 more sources