Results 51 to 60 of about 1,081,218 (337)
qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data
BMC Bioinformatics, 2022 Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-established method for analysing gene expression. Most RT-qPCR experiments in the field of microbiology aim for the detection of transcriptional changes by relative quantification ...
Daniel Flatschacher +2 more
semanticscholar +1 more source
Isoform-level gene signature improves prognostic stratification and accurately classifies glioblastoma subtypes. [PDF]
, 2014 Molecular stratification of tumors is essential for developing personalized therapies. Although patient stratification strategies have been successful; computational methods to accurately translate the gene-signature from high-throughput platform to a ...
Bi, Yingtao +5 more
core +1 more source
Large-Scale RT-qPCR Diagnostics for Seed Potato Certification [PDF]
Potato Research, 2021 AbstractEvery year, Agroscope examines nearly 300,000 tubers for the presence of viruses, as regulated for the certification of seed potatoes intended for Swiss growers. Since 2016, this examination has been performed via RT-qPCR on dormant tubers directly after harvest.
Olivier Schumpp +7 more
openaire +2 more sources
Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
FASEB BioAdvances, 2019 Extracellular microRNAs (miRs) have been proposed as important blood‐based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable and easily detectable in body fluids.
Maria Fauth +4 more
doaj +1 more source
Cross platform standardisation and normalisation experimental pipeline for use in the biodiscovery of dysregulated human circulating miRNAs [PDF]
, 2015 Introduction. Micro RNAs (miRNAs) are a class of highly conserved small non-coding RNAs that play an important part in the post-transcriptional regulation of gene expression.
Downing, Tim, Kelly, Helena
core +1 more source
Journal of Integrative Agriculture, 2014 Two microRNA (miRNA) quantification methods, namely, poly(A) reverse transcription (RT)-quantitative real-time polymerase chain reaction (qPCR) and stem-loop RT-qPCR, have been developed for quantifying miRNA expression. In the present study, five miRNAs,
Ran HAN +3 more
doaj +1 more source
Brazilian Journal of Infectious Diseases, 2023 Introdução/objetivos: As gastroenterites agudas (GEA) são doenças que se constituem como a segunda maior causa de morte em crianças de todo mundo, especialmente em crianças menores de cinco anos, ocorrendo principalmente em países de baixa e média renda.
Ana Luiza da Mota Raminho +5 more
doaj +1 more source
International Journal of Statistics in Medical Research, 2012 We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
Perkins, James R. +2 more
openaire +2 more sources
Custom RT-qPCR-array for glaucoma filtering surgery prognosis. [PDF]
PLoS ONE, 2017 Excessive subconjunctival scarring is the main reason of failure of glaucoma filtration surgery. We analyzed conjunctival and systemic gene expression patterns after non penetrating deep sclerectomy (NPDS). To find expression patterns related to surgical
Iñaki Rodriguez-Agirretxe +4 more
doaj +1 more source
Biological subtyping of early breast cancer : a study comparing RT-qPCR with immunohistochemistry [PDF]
, 2016 The biological subtype of breast cancer influences the selection of systemic therapy. Distinction between luminal A and B cancers depends on consistent assessment of Ki-67, but substantial intra-observer and inter-observer variability exists when ...
Auvinen, Paivi +14 more
core +2 more sources