Results 51 to 60 of about 491,020 (188)
Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
Advanced Science, 2023 The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic
Kiran Shrestha +18 more
doaj +1 more source
Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays [PDF]
, 2013 BACKGROUND: Human adenoviruses (HAdVs) are the second-leading cause of childhood gastroenteritis worldwide. This virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially UV light ...
Alessandra D’ A da Silva +6 more
core +1 more source
RT-qPCR Testing of SARS-CoV-2: A Primer [PDF]
International Journal of Molecular Sciences, 2020 Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR)
Stephen A. Bustin, Tania Nolan
openaire +4 more sources
Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR
Journal of Clinical Virology PlusBackground: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward.
David Alejandro Cabrera Gaytán +11 more
doaj +1 more source
Plants, 2022 C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C.
Luis Rubio +3 more
doaj +1 more source
Regulation of arginine transport by GCN2 eIF2 kinase is important for replication of the intracellular parasite Toxoplasma gondii [PDF]
, 2019 Toxoplasma gondii is a prevalent protozoan parasite that can infect any nucleated cell but cannot replicate outside of its host cell. Toxoplasma is auxotrophic for several nutrients including arginine, tryptophan, and purines, which it must acquire from ...
Amin, Parth H. +3 more
core +2 more sources
BMC Pediatrics, 2010 Background Neonatal sepsis is difficult to diagnose and pathogens cannot be detected from blood cultures in many cases. Development of a rapid and accurate method for detecting pathogens is thus essential.
Sato Hiroaki +10 more
doaj +1 more source
Algorithm Optimization in Methylation Detection with Multiple RT-qPCR. [PDF]
PLoS ONE, 2016 Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to ...
Lele Song +10 more
doaj +1 more source
How to perform RT-qPCR accurately in plant species?: a case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population [PDF]
, 2013 Background: Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids).
De Keyser, Ellen +3 more
core +2 more sources