Results 61 to 70 of about 1,081,218 (337)

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

PLoS ONE, 2021
Re-opening of communities in the midst of the ongoing COVID-19 pandemic has ignited new waves of infections in many places around the world. Mitigating the risk of reopening will require widespread SARS-CoV-2 testing, which would be greatly facilitated ...
T. G. Graham, C. Dugast-Darzacq, G. Dailey, X. Nguyenla, Erik Van Dis, Meagan N. Esbin, A. Abidi, S. Stanley, X. Darzacq, R. Tjian   +9 more
semanticscholar   +1 more source

Protocol-RT-qPCR v1

, 2021
Protocol-RT-qPCR to the paper: Viral mapping in COVID-19 deceased in the Augsburg autopsy series of the first wave: a multiorgan and multimethodological approach Full organ mapping of patients with COVID-19: an autopsy study
openaire   +1 more source

TaqMan RT-qPCR for tRNA half quantification. [PDF]

Methods Enzymol
When quantifying tRNA-derived short non-coding RNAs (sncRNAs), two key considerations must be addressed. First, sequencing analyses have revealed significant heterogeneity in the lengths and terminal sequences of tRNA-derived sncRNAs. Second, within the total RNA fraction, these sncRNAs coexist with more abundant mature tRNAs and their precursors (pre ...
Shigematsu M, Kawamura T, Kirino Y.
europepmc   +3 more sources

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]

, 2012
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Kaiser, Sebastian, Meyer, Swanhild U., Pfaffl, Michael W., Thirion, Christian, Wagner, Carola   +4 more
core   +1 more source

Identification and validation of extracellular vesicle reference genes for the normalization of RT‐qPCR data

Journal of Extracellular Vesicles
Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with diverse diagnostic and therapeutic application potential.
Cláudio Pinheiro, Niké Guilbert, L. Lippens, Quentin Roux, Robin Boiy, Suzanne Fischer, Sofie Van Dorpe, Bram De Craene, G. Berx, T. Boterberg, G. Sys, H. Denys, I. Miinalainen, P. Mestdagh, J. Vandesompele, O. De Wever, An Hendrix   +16 more
semanticscholar   +1 more source

Prognostic value of CIP2A gene expression in adult Egyptian acute myeloid leukemia patients

Egyptian Journal of Medical Human Genetics, 2020
Background Protein phosphatase 2A (PP2A) functions as a tumor suppressor in many cancers. Cancerous inhibitor of protein phosphatase 2A (CIP2A) inhibits PP2A proteolytic degradation of c-Myc and enhances cell growth and tumor formation in several tissues.
Roxan E. Shafik, Azza M. Ibrahim, Fadwa Said, Naglaa M. Hassan, Hanan E. Shafik, Hala A. Shokralla   +5 more
doaj   +1 more source

TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways [PDF]

, 2016
Primer Sequences used for RT-qPCR (5’ to 3’).
Hui-Ying Sun, Jia-Huan Zhang, Jun-Feng Qiu, Li Liang, Mei-Rong He, Meng Wang, Ming-Xuan Li, Run-Wei Yang, Sheng-Lu Jiang, Wen-Ting Liao, Wen-Ting Wei, Xin-Xin Nian, Ya-Li Zhao, Yan-Mei Cui, Yan-Qing Ding, Ying-Yue Zeng, Yu-Ping Quan, Yue-Long Cai, Yun-Teng Hu   +18 more
core   +5 more sources

RT-qPCR v1

, 2018

Thomas P Howard, Taylor Arnoff
openaire   +1 more source

Regulation of arginine transport by GCN2 eIF2 kinase is important for replication of the intracellular parasite Toxoplasma gondii [PDF]

, 2019
Toxoplasma gondii is a prevalent protozoan parasite that can infect any nucleated cell but cannot replicate outside of its host cell. Toxoplasma is auxotrophic for several nutrients including arginine, tryptophan, and purines, which it must acquire from ...
Amin, Parth H., Augusto, Leonardo, Sullivan, William J., Wek, Ronald C.   +3 more
core   +2 more sources

Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR

Plant Methods, 2022
Background Seeds were an important medium for long-distance transmission of plant viruses. Therefore, appropriate, more sensitive methods for detecting low concentrations of virus-infected in seeds were crucial to ensure the quality of seed lots. In this
Yin Xinying, Li Xin, Yang Lili, Zheng Qiuyue, Piao Yongzhe, Cao Jijuan   +5 more
doaj   +1 more source