BMC Infectious Diseases, 2022 Background The COVID-19 pandemic remains the main public health problem, due to the quick and easy dissemination of the causal agent, SARS-CoV-2 virus, around the world.
Diana Flores-León +5 more
doaj +1 more source
Evaluation of eleven reference genes for Reverse Transcriptase Quantitative PCR of rubber tree under water deficit [PDF]
, 2015 Reverse Transcriptase Quantitative PCR (RTqPCR) is a powerful technique in order to detect low abundance of mRNA in the plant cell. The measurement of transcript abundance is relative to the control of expression such as housekeeping genes.
Leclercq, Julie +2 more
core
The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing [PDF]
, 2011 Background The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo, mainly relating to inefficient delivery and non-specific effects of short RNA ...
Mike Herbert +4 more
core +2 more sources
TaqMan RT-qPCR for tRNA half quantification. [PDF]
Methods EnzymolWhen quantifying tRNA-derived short non-coding RNAs (sncRNAs), two key considerations must be addressed. First, sequencing analyses have revealed significant heterogeneity in the lengths and terminal sequences of tRNA-derived sncRNAs. Second, within the total RNA fraction, these sncRNAs coexist with more abundant mature tRNAs and their precursors (pre ...
Shigematsu M, Kawamura T, Kirino Y.
europepmc +3 more sources
Levels of feline infectious peritonitis virus in blood, effusions, and various tissues and the role of lymphopenia in disease outcome following experimental infection. [PDF]
, 2015 Twenty specific pathogen free cats were experimentally infected with a virulent cat-passaged type I field strain of FIPV. Eighteen cats succumbed within 2-4 weeks to effusive abdominal FIP, one survived for 6 weeks, and one seroconverted without outward ...
Eckstrand, Chrissy +4 more
core +2 more sources
Hepatitis A virus subgenotyping based on RT-qPCR assays [PDF]
BMC Microbiology, 2014 The hepatitis A virus (HAV) is the most frequent cause of viral hepatitis worldwide and is recognized as one of the most widespread foodborne pathogens. HAV genotypes and subtypes differ in their geographic distribution and the incidence of HAV infection varies considerably among countries, and is particularly high in areas with poor sanitation and ...
Coudray-Meunier, Coralie +5 more
openaire +2 more sources
The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region [PDF]
, 2019 BACKGROUND: The cell-cycle inhibitor p57kip2 plays a critical role in mammalian development by coordinating cell proliferation and differentiation in many cell types.
Andresini, Oriella +5 more
core +4 more sources
, 2018 RNA from matched FFPE, PFPE and cryo preserved human tissues, stored for up to seven years at 22°C and 4°C, examined for integrity and usability in quantitative RT-PCR
openaire +1 more source
RT-qPCR Detection of Senescence-Associated Circular RNAs [PDF]
, 2016 Primary cells that reach the end of their replicative potential, encounter sublethal stress, or experience the activation of certain oncogenes cease proliferation and enter a state of long-term growth arrest named senescence. The senescent process has been implicated in a variety of age-related diseases and also in the pathogenesis of cancer ...
Amaresh C, Panda +2 more
openaire +2 more sources
Development and characterization of a new human hepatic cell line [PDF]
, 2015 The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines.
Berx, Geert +6 more
core +3 more sources