Results 71 to 80 of about 491,020 (188)

RT-qPCR v1

, 2017

openaire   +1 more source

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR.

PLoS ONE
IntroductionDengue fever is a growing global concern with an estimated 100-400 million infections every year and rising mortality over the past decade. In 2017, 40,000 deaths were attributed to dengue. Real-time reverse transcription PCR (RT-qPCR) is the
Vilayouth Phimolsannousith, Malavanh Vongsouvath, Padthana Kiedsathid, Manivanh Vongsouvath, Elizabeth A Ashley, Audrey Dubot-Pérès   +5 more
doaj   +1 more source

Simultaneous detection of human norovirus GI, GII and SARS-CoV-2 by a quantitative one-step triplex RT-qPCR

Frontiers in Microbiology
BackgroundThere are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-
Hua-Wei Yi, Hua-Wei Yi, Xian-Mo Wang, Xian-Mo Wang, Xin Tan, Cai-Zhi Ding, Chang-Li Zhang, Chang-Li Zhang, Jia-Hao Wu, Jia-Hao Wu, Qi Li, Qi Li, Chen-Qi Xin, Chen-Qi Xin, Wen Fan, Wen Fan   +15 more
doaj   +1 more source

RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings

Bio-Protocol, 2020
Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions.
Tianran Jia, Brandon Le
doaj   +1 more source

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

The EuroBiotech Journal, 2021
A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2.
Tuncel Gulten, Ergoren Mahmut Cerkez, Baddal Buket, Tulay Pinar, Arikan Ayse, Guler Emrah, Ozverel Cenk Serhan, Kaya Suer H., Sayan Murat, Sanlidag Tamer,   +10 more
doaj   +1 more source

The Long Noncoding RNA HEAL Regulates HIV-1 Replication through Epigenetic Regulation of the HIV-1 Promoter. [PDF]

, 2019
A major challenge in finding a cure for HIV-1/AIDS is the difficulty in identifying and eradicating persistent reservoirs of replication-competent provirus.
Chang, Kungyen, Chao, Ti-Chun, Karris, Maile Ann Young, Kaul, Marcus, Li, Zhonghan, Qin, Yue, Rana, Tariq M, Sanchez, Ana, Singh, Gatikrushna, Tiwari, Shashi Kant, Yau, Edwin, Zhang, Qiong   +11 more
core   +1 more source

RT-qPCR v1

Gene expression levels by RT-qPCR
Karyna Tarasova, Sinan Gültekin, Angkana Kidtiwong, iris.gerner Gerner, Florien Jenner   +4 more
openaire   +1 more source

RNA Extraction and RT-qPCR v1

, 2022
This protocol is used to extract RNA from mouse brain tissue and quantify with RT-qPCR.
openaire   +1 more source

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

JMIR Public Health and Surveillance
BackgroundMany countries have applied the wastewater surveillance of the COVID-19 pandemic to their national public health monitoring measures.
Annika Länsivaara, Kirsi-Maarit Lehto, Rafiqul Hyder, Erja Sinikka Janhonen, Anssi Lipponen, Annamari Heikinheimo, Tarja Pitkänen, Sami Oikarinen   +7 more
doaj   +1 more source

Heterogeneous expression of EPCAM in human circulating tumour cells from patient-derived xenografts

Biomarker Research, 2018
Background We aim to characterize the heterogeneous circulating tumour cells (CTCs) in peripheral blood, independently of physical or immunological purification, by using patient-derived xenografts (PDXs) models.
Chiara Agnoletto, Linda Minotti, Laura Brulle-Soumare, Lorenzo Pasquali, Marco Galasso, Fabio Corrà, Federica Baldassari, Jean-Gabriel Judde, Stefano Cairo, Stefano Volinia   +9 more
doaj   +1 more source