Results 81 to 90 of about 1,081,218 (337)

RAW data for RT-qPCR

, 2023
RAW data for RT ...
openaire   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR

Journal of Clinical Virology Plus
Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward.
David Alejandro Cabrera Gaytán, Porfirio Felipe Hernández Bautista, Clara Esperanza Santacruz Tinoco, Julio Elias Alvarado Yaah, Yu-Mei Anguiano Hernández, Bernardo Martínez Miguel, Alfonso Vallejos Parás, Lumumba Arriaga Nieto, Nancy Sandoval Gutiérrez, Concepción Grajales Muñiz, Alejandro Moctezuma Paz, Leticia Jaimes Betancourt   +11 more
doaj   +1 more source

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR. [PDF]

PLoS ONE, 2016
Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to ...
Lele Song, Yuemin Li, Jia Jia, Guangpeng Zhou, Jianming Wang, Qian Kang, Peng Jin, Jianqiu Sheng, Guoxiang Cai, Sanjun Cai, Xiaoliang Han   +10 more
doaj   +1 more source

The dual nature of trehalose in citrus canker disease: A virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses [PDF]

, 2015
Xanthomonas citri subsp. citri (Xcc) is a bacterial pathogen that causes citrus canker in susceptible Citrus spp. The Xcc genome contains genes encoding enzymes from three separate pathways of trehalose biosynthesis.
Feil, Regina, Ficarra, Florencia Andrea, Garavaglia, Betiana Soledad, Gehring, Chris, Gottig Schor, Natalia, Lunn, John E., Marondedze, Claudius, Ottado, Jorgelina, Piazza, Ainelén Melanie, Thomas, Ludivine, Zimaro, Tamara   +10 more
core   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: a case control study

BMC Pediatrics, 2010
Background Neonatal sepsis is difficult to diagnose and pathogens cannot be detected from blood cultures in many cases. Development of a rapid and accurate method for detecting pathogens is thus essential.
Sato Hiroaki, Shoji Hiromichi, Komatsu Mitsutaka, Matsunaga Nobuaki, Nagata Satoru, Hisata Ken, Fujimori Makoto, Yamashiro Yuichiro, Asahara Takashi, Nomoto Koji, Shimizu Toshiaki   +10 more
doaj   +1 more source

Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing

Plants, 2022
C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C.
Luis Rubio, Francisco J. J. Guinot-Moreno, Carmen Sanz-López, Luis Galipienso   +3 more
doaj   +1 more source

Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays [PDF]

, 2013
BACKGROUND: Human adenoviruses (HAdVs) are the second-leading cause of childhood gastroenteritis worldwide. This virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially UV light ...
Alessandra D’ A da Silva, Caroline Rigotto, Célia R M Barardi, Giseli Ritterbusch, Gislaine Fongaro, Mariana A do Nascimento, Paulo A Esteves   +6 more
core   +1 more source

Validation of Circular RNAs Using RT‐qPCR After Effective Removal of Linear RNAs by Ribonuclease R

Current Protocols, 2021
Circular RNAs (circRNAs) are a class of endogenous noncoding RNAs that have been shown to play a role in normal development, homeostasis, and disease, including cancer.
Marieke Vromman, N. Yigit, K. Verniers, Steve Lefever, J. Vandesompele, P. Volders   +5 more
semanticscholar   +1 more source