Results 81 to 90 of about 491,020 (188)

Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats [PDF]

, 2014
BACKGROUND: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR ...
Balkema-Buschmann, Anne, Beer, Martin, Chriél, Mariann, Fischer, Melina, Freuling, Conrad M., Hoffmann, Bernd, Müller, Thomas, Rasmussen, Thomas Bruun, Schatz, Juliane   +8 more
core   +1 more source

Linear-hairpin variable primer RT-qPCR for MicroRNA

Chemical Science, 2019
Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR.
Lin Lan, Qiuping Guo, Hemin Nie, Chang Zhou, Qingyun Cai, Jin Huang, Xiangxian Meng   +6 more
openaire   +3 more sources

Improving the standardization of mRNA measurement by RT-qPCR [PDF]

Biomolecular Detection and Quantification, 2018
Human health and safety depend on reliable measurements in medical diagnosis and on tests that support the selection and evaluation of therapeutic intervention and newly discovered molecular biomarkers must pass a rigorous evaluation process if they are to be of benefit to patients.
Rebecca Sanders, Stephen Bustin, Jim Huggett, Deborah Mason   +3 more
openaire   +3 more sources

Canine distemper virus detection by different methods of One-Step RT-qPCR

Ciência Rural, 2016
: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical ...
Claudia de Camargo Tozato, Vívian Ferreira Zadra, Caroline Rodrigues Basso, João Pessoa Araújo Junior   +3 more
doaj   +1 more source

Bta-miR-10b secreted by bovine embryos negatively impacts preimplantation embryo quality [PDF]

, 2019
In a previous study, we found miR-10b to be more abundant in a conditioned culture medium of degenerate embryos compared to that of blastocysts. Here, we show that miR-10b mimics added to the culture medium can be taken up by embryos. This uptake results
Deforce, Dieter, Heindryckx, Björn, Lin, Xiaoyuan, Pavani, Krishna, Peelman, Luc, Smits, Katrien, Van Soom, Ann   +6 more
core   +1 more source

Characterization of Zika virus endocytic pathways in human glioblastoma cells [PDF]

, 2020
Zika virus (ZIKV) infections can cause microcephaly and neurological disorders. However, the early infection events of ZIKV in neural cells remain to be characterized. Here, by using a combination of pharmacological and molecular approaches and the human
Du, T, Fu, M, Griffin, GE, Hu, Q, Li, C, Li, M, Liu, Y, Ni, F, Wang, H, Zhang, D, Zhang, M, Zheng, Z   +11 more
core   +1 more source

Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms

Frontiers in Genetics, 2018
MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3′-end length variants
Charlotte Nejad, Charlotte Nejad, Geneviève Pépin, Geneviève Pépin, Mark A. Behlke, Michael P. Gantier, Michael P. Gantier   +6 more
doaj   +1 more source

WetLab-2 RT-qPCR procedure v2

, 2017
Procedure used for RT-qPCR in the WetLab-2 ...
Jimmy Jung, Macarena Parra
openaire   +1 more source

Diagnostic value of reverse transcription-quantitative PCR in skin pus specimens for HIV-negative talaromycosis marneffei

Guangxi Yike Daxue xuebao
Objective To evaluate the diagnostic value of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) detection of skin pus specimens in HIV-negative patients with talaromycosis marneffei (TSM).
Lan HUANG, Qihua HUANG, Dongyan ZHENG, Weixuan WU, Kaisu PAN, Yanqing ZHENG, Wanqing LIAO, Cunwei CAO   +7 more
doaj   +1 more source

Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR

Molecular Cytogenetics, 2012
Background ZAP70 gene expression is associated with poor prognosis in B-cell lymphoproliferative disorders especially chronic lymphocytic leukaemia (CLL) but its role in adult B-ALL has not been established.
Chakupurakal Geothy, Bell Andrew, Griffiths Mike, Wandroo Farooq, Moss Paul   +4 more
doaj   +1 more source