Results 11 to 20 of about 191 (49)

Site-specific analysis of protein S-acylation by resin-assisted capture[S]

Journal of Lipid Research, 2011
Protein S-acylation is a major posttranslational modification whereby a cysteine thiol is converted to a thioester. A prototype is S-palmitoylation (fatty acylation), in which a protein undergoes acylation with a hydrophobic 16 carbon lipid chain ...
Michael T. Forrester, Douglas T. Hess, J. Will Thompson, Rainbo Hultman, M. Arthur Moseley, Jonathan S. Stamler, Patrick J. Casey   +6 more
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In vitro reconstitution of substrate S-acylation by the zDHHC family of protein acyltransferases

Open Biology, 2022
Protein S-acylation, more commonly known as protein palmitoylation, is a biological process defined by the covalent attachment of long chain fatty acids onto cysteine residues of a protein, effectively altering the local hydrophobicity and influencing ...
R. Elliot Murphy, Anirban Banerjee
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Assaying protein palmitoylation in plants

Plant Methods, 2008
Background Protein S-acylation (also known as palmitoylation) is the reversible post-translational addition of acyl lipids to cysteine residues in proteins through a thioester bond. It allows strong association with membranes.
Taylor Laura, Hemsley Piers A, Grierson Claire S   +2 more
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Efficient S-Acylation of Thiourea

SynOpen, 2018
Efficient S-acylation of thiourea using a variety of acid chlorides is reported. Structurally diverse aryl and alkyl substrates are compatible with this methodology.
David J. Jones, Uday B. Rao Khandavilli, Eileen M. O’Leary, Simon E. Lawrence, Timothy P. O’Sullivan   +4 more
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2-Bromopalmitate reduces protein deacylation by inhibition of acyl-protein thioesterase enzymatic activities. [PDF]

PLoS ONE, 2013
S-acylation, the covalent attachment of palmitate and other fatty acids on cysteine residues, is a reversible post-translational modification that exerts diverse effects on protein functions.
Maria P Pedro, Aldo A Vilcaes, Vanesa M Tomatis, Rafael G Oliveira, Guillermo A Gomez, Jose L Daniotti   +5 more
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S-acylation dependent post-translational cross-talk regulates large conductance calcium- and voltage- activated potassium (BK) channels

Frontiers in Physiology, 2014
Mechanisms that control surface expression and/or activity of large conductance calcium-activated potassium (BK) channels are important determinants of their (patho)physiological function.
Michael J Shipston
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Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) are membrane-enclosed particles that play an important role in cancer progression and have emerged as a promising source of circulating biomarkers. Protein S-acylation, frequently called palmitoylation, has been proposed as a
Javier Mariscal, Tatyana Vagner, Minhyung Kim, Bo Zhou, Andrew Chin, Mandana Zandian, Michael R. Freeman, Sungyong You, Andries Zijlstra, Wei Yang, Dolores Di Vizio   +10 more
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Dynamics of CLIMP-63 S-acylation control ER morphology

Nature Communications, 2023
A key player in the formation of endoplasmic reticulum sheets is CLIMP-63, but mechanistic details remained elusive. Here authors combined cellular experiments and mathematical modelling to show that S-acylation of CLIMP-63 regulates its function by ...
Patrick A. Sandoz, Robin A. Denhardt-Eriksson, Laurence Abrami, Luciano A. Abriata, Gard Spreemann, Catherine Maclachlan, Sylvia Ho, Béatrice Kunz, Kathryn Hess, Graham Knott, Francisco S. Mesquita, Vassily Hatzimanikatis, F. Gisou van der Goot   +12 more
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Dynamic acylome reveals metabolite driven modifications in Syntrophomonas wolfei

Frontiers in Microbiology, 2022
Syntrophomonas wolfei is an anaerobic syntrophic microbe that degrades short-chain fatty acids to acetate, hydrogen, and/or formate. This thermodynamically unfavorable process proceeds through a series of reactive acyl-Coenzyme A species (RACS). In other
Janine Y. Fu, John M. Muroski, Mark A. Arbing, Jessica A. Salguero, Neil Q. Wofford, Michael J. McInerney, Robert P. Gunsalus, Robert P. Gunsalus, Robert P. Gunsalus, Joseph A. Loo, Joseph A. Loo, Joseph A. Loo, Joseph A. Loo, Rachel R. Ogorzalek Loo, Rachel R. Ogorzalek Loo, Rachel R. Ogorzalek Loo   +15 more
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Metabolically controlled histone H4K5 acylation/acetylation ratio drives BRD4 genomic distribution

Cell Reports, 2021
Summary: In addition to acetylation, histones are modified by a series of competing longer-chain acylations. Most of these acylation marks are enriched and co-exist with acetylation on active gene regulatory elements.
Mengqing Gao, Jin Wang, Sophie Rousseaux, Minjia Tan, Lulu Pan, Lijun Peng, Sisi Wang, Wenqian Xu, Jiayi Ren, Yuanfang Liu, Martin Spinck, Sophie Barral, Tao Wang, Florent Chuffart, Ekaterina Bourova-Flin, Denis Puthier, Sandrine Curtet, Lisa Bargier, Zhongyi Cheng, Heinz Neumann, Jian Li, Yingming Zhao, Jian-Qing Mi, Saadi Khochbin   +23 more
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