Results 181 to 190 of about 333,929 (218)
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Cloning, characterization and overproduction of nuclease S1 gene (nucS) from Aspergillus oryzae

Applied Microbiology and Biotechnology, 1995
The nuclease S1 gene (nucS) from Aspergillus oryzae was isolated using a polymerase-chain-reaction-amplified DNA fragment as a probe, and a 2.6-kb SalI-EcoRI fragment containing the nucS gene was sequenced. It was deduced that the nucS gene had two short introns, 49 and 50 nucleotides in length. The nucS gene had an open-reading frame of 963 base pairs
B R, Lee   +3 more
openaire   +2 more sources

Ribosomal Protein S1(RpsA) of Buchnera aphidicola , the Endosymbiont of Aphids: Characterization of the Gene and Detection of the Product

Current Microbiology, 1996
Buchnera aphidicola is a prokaryotic endosymbiont found in specialized cells of the aphid Schizaphis graminum. Many of the previously cloned B. aphidicola genes are preceded by a poor ribosome-binding site. Ribosomal protein S1 (RpsA) allows the translation of messenger RNAs that lack or have a poor ribosome binding site. We have cloned and sequenced a
M A, Clark   +3 more
openaire   +2 more sources

Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain

Applied Microbiology and Biotechnology, 2013
Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is a highly contagious chicken disease, and can lead to serious economic losses in poultry enterprises. The continual introduction of new IBV serotypes requires alternative strategies for the production of timely and safe vaccines against the emergence of variants. Modification of
Yan-Quan, Wei   +9 more
openaire   +2 more sources

Isolation and characterization of mutants with impaired regulation of rpsA, the gene encoding ribosomal protein S1 of Escherichia coli

Molecular and General Genetics MGG, 1993
In order to select mutants that would help to characterize the post-transcriptional regulation of rpsA, we constructed a strain in which the growth rate on lactose minimal medium is determined by the amount of an rpsA-lacZ' alpha-fragment fusion protein produced, even when this is encoded by a high-copy-number plasmid. In the parental strain, synthesis
Rasmussen, M.D.   +2 more
openaire   +2 more sources

Development of gene transfer methods forRubrivivax gelatinosus S1: construction, characterization and complementation of apuf operon deletion strain

Molecular and General Genetics MGG, 1996
Gene transfer systems were developed in Rubrivivax (Rx.) gelatinosus S1. First, a system for conjugative transfer of mobilizable plasmids from Escherichia coli to Rx. gelatinosus S1 was established. Secondly, optimal conditions for the transformation of Rx. gelatinosus S1 by electroporation were determined.
S, Ouchane   +4 more
openaire   +2 more sources

Molecular cloning and characterization of buffalo alpha(s1)-casein gene.

DNA sequence : the journal of DNA sequencing and mapping, 2008
Buffaloes in Indian subcontinent play an important role as the producer of milk and milk products. The alpha(s1)-casein constitutes 38% of the total milk proteins. The present study was carried out to characterize the gene in Murrah breed of Riverine buffalo.
S, Sukla   +4 more
openaire   +1 more source

Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries: identification of intertypic recombination in the S1 gene

Avian Pathology, 2011
Infectious bronchitis virus (IBV) isolates recovered in Russia, Ukraine, and Kazakhstan between 2007 and 2010 were subjected to molecular characterization and compared with those isolated a decade ago. The IBV genome was detected in 202 out of 605 field samples from chickens with various clinical signs.
Evgeniya V, Ovchinnikova   +8 more
openaire   +2 more sources

Molecular Characterization and Expression Analysis of S1 self‐incompatibility Locus‐linked Pollen 3.15 Gene in Citrus reticulata

Journal of Integrative Plant Biology, 2013
AbstractGametophytic self‐incompatibility (GSI) is controlled by a highly polymorphic locus called the S‐locus, which is an important factor that can result in seedless fruit in Citrus. The S1 self‐incompatibility locus‐linked pollen 3.15 gene (S1‐3.15) belongs to a type of S locus gene. The role of S1‐3.15 in the SI reaction of Citrus has not yet been
Hong Xia, Miao   +3 more
openaire   +2 more sources

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