Results 111 to 120 of about 260,956 (156)
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Classical NLS Proteins from Saccharomyces cerevisiae
Journal of Molecular Biology, 2008Proteins can enter the nucleus through various receptor-mediated import pathways. One class of import cargos carries a classical nuclear localization signal (cNLS) containing a short cluster of basic residues. This pathway involves importin alpha (Impalpha), which possesses the cNLS binding site, and importin beta (Impbeta), which translocates the ...
Silvia, Hahn +3 more
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Protein arginine methylation in Saccharomyces cerevisiae
The FEBS Journal, 2012Recent research has implicated arginine methylation as a major regulator of cellular processes, including transcription, translation, nucleocytoplasmic transport, signalling, DNA repair, RNA processing and splicing. Arginine methylation is evolutionarily conserved, and it is now thought that it may rival other post‐translational modifications such as ...
Jason K K, Low, Marc R, Wilkins
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Rapid protein extraction from Saccharomyces cerevisiae
Yeast, 1994AbstractWe have developed and evaluated an easy and rapid method for extraction of proteins from yeast cells for sodium dodecyl sulphate (SDS)–gel electrophoresis and Western blotting. The procedure comprises a centrifugation step to harvest the cells, addition of a sample buffer and heating, then another centrifugation step before applying the ...
A, Horvath, H, Riezman
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Saccharomyces Cerevisiae Strains that Overexpress Heterologous Proteins
Nature Biotechnology, 1991We describe a system that facilitates the selection of host mutants that overproduce a range of secreted and internally produced heterologous proteins in Saccharomyces cerevisiae. These mutants were initially selected for their ability to oversecrete recombinant human albumin (rHA), as detected by a direct visual assay that relies upon antibody ...
D, Sleep +7 more
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Assaying protein ubiquitination in Saccharomyces cerevisiae
2002Publisher Summary The covalent modification of target proteins by the polypeptide ubiquitin (Ub) is involved in a wide array of cellular processes, ranging from cell cycle progression and receptor-mediated endocytosis to endoplasmic reticulum-associated degradation and cell-type specification.
Jeffrey D, Laney, Mark, Hochstrasser
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High mobility group proteins of Saccharomyces cerevisiae
Biochemistry, 1980The yeast Saccharomyces cerevisiae contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but it is eluted by 0.25 N HCl. It follows that HMGs cannot, in general, be defined by extractability criteria.
S, Weber, I, Isenberg
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UV-inducible proteins in Saccharomyces cerevisiae
Current Genetics, 1985Two UV-inducible proteins have been detected in the yeast, Saccharomyces cerevisiae. The proteins have molecular weights of 78,000 Daltons and 23,000 Daltons. This induction is specific for UV-irradiation as exposure to X-rays, mitomycin C and heat shock does not result in the synthesis of the proteins.
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Scarless Genomic Protein Labeling in Saccharomyces cerevisiae
2020Labeling a protein of interest is widely used to examine its quantity, modification, localization, and dynamics in the budding yeast Saccharomyces cerevisiae. Fluorescent proteins and epitope tags are often used as protein fusion tags to study target proteins.
Qian, Wang, Yu V, Fu, Wei, Xiao
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Saccharomyces cerevisiae Ssb proteins
1997Abstract The SSB1, SSB2 genes (initially known as YG101, YG103) were isolated by virtue of their high degree of similarity to Drosophila HSP70 genes (Ingolia et al., 1982; Craig, Jacobsen, 1985).
T Ziegelhoffer, E A Craig
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Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae
2022Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to MP characterization include ample overexpression, solubilization, and purification of target proteins while maintaining native activity and structure.
Landon, Haslem +4 more
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