Results 111 to 120 of about 274,580 (343)
Identification of Essential Proteins Using Induced Stars in Protein-Protein Interaction Networks [PDF]
arXiv, 2017 In this work, we propose a novel centrality metric, referred to as star centrality, which incorporates information from the closed neighborhood of a node, rather than solely from the node itself, when calculating its topological importance. More specifically, we focus on degree centrality and show that in the complex protein-protein interaction ...
arxiv
Journal of Cell Biology, 1996 We identified a Saccharomyces cerevisiae peroxisomal membrane protein, Pex13p, that is essential for protein import. A point mutation in the COOH-terminal Src homology 3 (SH3) domain of Pex13p inactivated the protein but did not affect its membrane ...
Y. Elgersma +8 more
semanticscholar +1 more source
Single Cell Protein Production From Ethanol: Model‐Based Bioreactor Operation at Industrial Scale
Biotechnology and Bioengineering, EarlyView.ABSTRACT Alternative fermentation feedstocks such as ethanol can be produced from CO2 via electrocatalytic processes that coproduce O2. In this study, industrial‐scale fermentation of ethanol with pure O2 for single cell protein (SCP) production was studied using a modeling approach.
Eduardo Almeida Benalcázar +6 more
wiley +1 more source
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for protein concentration in different compartments of single living cells, validated using realistic ...
arxiv +1 more source
Molecular and Cellular Biology, 1996 Mcm2, Mcm3, and Mcm5/Cdc46 are conserved proteins essential for the initiation of DNA synthesis at replication origins in Saccharomyces cerevisiae. The accumulation of these proteins in the nucleus before the onset of DNA synthesis suggests that they ...
Ming Lei, Yasuo Kawasaki, B. Tye
semanticscholar +1 more source
Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments
Biotechnology and Bioengineering, EarlyView.The authors investigated how three Saccharomyces cerevisiae strains respond to dynamic substrate and pH oscillations using dynamic microfluidic single‐cell cultivation (dMSCC) and intracellular biosensors. By combining performance and robustness analysis of selected intracellular parameters, growth and morphological analysis, the authors identified ...
Luca Torello Pianale +3 more
wiley +1 more source
Microbial Biotechnology, 2019 Summary Heterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well‐established and widely used technique.
Mario González +3 more
doaj +1 more source
Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
Bio-Protocol, 2019 Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial step,
Laura Knighton +3 more
doaj +1 more source
Bioinformatics analysis of experimentally determined protein complexes in the yeast, S. cerevisiae [PDF]
arXiv, 2003 Many important cellular functions are implemented by protein complexes that act as sophisticated molecular machines of varying size and temporal stability. Here we demonstrate quantitatively that protein complexes in the yeast, Saccharomyces cerevisiae, are comprised of a core in which subunits are highly coexpressed, display the same deletion ...
arxiv
RNase H2 of Saccharomyces cerevisiae is a complex of three proteins.
Nucleic Acids Research, 2004 The composition of RNase H2 has been a long-standing problem. Whereas bacterial and archaeal RNases H2 are active as single polypeptides, the Saccharomyces cerevisiae homolog, Rnh2Ap, when expressed in Escherichia coli, fails to produce an active RNase ...
H. Jeong +4 more
semanticscholar +1 more source