Results 71 to 80 of about 13,319,037 (325)

A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells (Adv. Biology 10/2023) [PDF]

, 2023
Antentor Hinton, Prasanna Katti, Trace Christensen, Margaret Mungai, Jianqiang Shao, Liang Zhang, Sergey Trushin, Ahmad F. Alghanem, Adam Jaspersen, Rachel E. Geroux, Kit Neikirk, Michelle Biete, Edgar Garza-López, Bryanna Shao, Zer Vue, Larry Vang, Heather K. Beasley, Andrea G. Marshall, Dominique Stephens, Steven M. Damo, Jessica Ponce, Christopher K. E. Bleck, Innes Hicsasmaz, Sandra Murray, Ranthony A. C. Edmonds, Andres Dajles, Young Do Koo, Serif Bacevac, Jeffrey L. Salisbury, Renata O. Pereira, Brian Glancy, Eugenia Trushina, E. Dale Abel   +32 more
openalex   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Sample preparation for routine and advanced structural biology, including serial data collection, MicroED and cryo-EM [PDF]

, 2023
Stefan Kolek, Patrick D. Shaw Stewart, Jack Stubbs, Peter Baldock   +3 more
openalex   +1 more source

Automated sample preparation with SP3 for low‐input clinical proteomics

bioRxiv, 2019
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including fresh frozen tissue, FFPE tissue, or blood.
T. Müller, Mathias Kalxdorf, Rémi Longuespée, D. Kazdal, A. Stenzinger, J. Krijgsveld   +5 more
semanticscholar   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Sample Preparation

Sample preparation. Documentation of the sample preparation of samples from year 2.
Jacqueline A. Hubbard, Judith A. Stone, J. Grace van der Gugten   +2 more
  +6 more sources

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Toxins, 2020
As a class of mycotoxins with regulatory and public health significance, aflatoxins (e.g., aflatoxin B1, B2, G1 and G2) have attracted unparalleled attention from government, academia and industry due to their chronic and acute toxicity.
Kai Zhang, K. Banerjee
semanticscholar   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source