Results 241 to 250 of about 1,812,833 (310)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Performance of Artificial Intelligence Systems for Automated Segmentation and Quantification of Retinal Fluid and Pathology in Optical Coherence Tomography Scans: A Systematic Review and Meta-Analysis. [PDF]

Cureus
Awan B, Elsaigh M, Hesham Gamal M, Elbahnasawy SE, Badee M.   +4 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Application of Quantitative PCR (qPCR) for the Detection of Psittacine Beak and Feather Disease Virus (PBFDV) in Air Conditioning Systems: A Model Study from a Veterinary Hospital in Thailand. [PDF]

Vet Sci
Lorsunyaluck B, Charachit J, Putsetkun S, Pumipuntu N.   +3 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Distance-Restraint-Guided Diffusion Models for Sampling Protein Conformational Changes and Ligand Dissociation Pathways. [PDF]

J Chem Theory Comput
Hori T, Moriwaki Y, Ishitani R.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Genomic Epidemiology of Antibiotic-Resistant Bacteria Sampled from Metropolitan Wastewater. [PDF]

Microorganisms
Deslouches JT, Raabe NJ, Mills EG, Fleres G, Wallace NR, Yassin MH, Van Tyne D.   +6 more
europepmc   +1 more source

How nature discovers rare Turing islands: Exploration by common limit cycles. [PDF]

Proc Natl Acad Sci U S A
Kim S, Matas-Gil A, Endres RG.
europepmc   +1 more source

Comparison of Protein-Glycosaminoglycan Interactions in ff14sb/GLYCAM06j-1 and CHARMM36m Force Fields. [PDF]

J Chem Inf Model
Bojarski KK, Wesołowski PA, Dewan D, Dziadek ŁJ, Neuman V, Brooks BR, Czub J, Zacharias M, Sieradzan AK, Wales DJ.   +9 more
europepmc   +1 more source