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Influence of the administration route and dose on the expression and antibody responses of a reporter and avian influenza self-amplifying mRNA vaccine in poultry [PDF]

Veterinary Quarterly
Vaccination is routinely used in industrial poultry to control infectious diseases. Vaccines based on mRNA and self-amplifying RNA (saRNA) are approved for human use, but research on their application in poultry is limited.
Janne Snoeck, Xiaole Cui, Pieter Vervaeke, Niek N. Sanders, An Garmyn   +4 more
doaj   +2 more sources

Optimization of In Vitro Transcription by Design of Experiment to Achieve High Self-Amplifying RNA Integrity [PDF]

Vaccines
Background: Self-amplifying mRNA (saRNA) holds promising application prospects. However, due to the inclusion of a replicase sequence, its extended length leads to premature termination during in vitro transcription (IVT), resulting in poor product ...
Chaoying Hu, Haixin Wang, Guanxing Liu, Kelei Li, Xuanxuan Zhang, Lifang Song, Fan Gao, Xing Wu, Qian Wang, Mingchen Liu, Jianyang Liu, Zhihao Fu, Xiao Ma, Miao Xu, Qunying Mao, Zhenglun Liang, Qian He   +16 more
doaj   +2 more sources

Impact of pre-existing anti-replicase immunity on the efficacy of self-amplifying mRNA vaccines [PDF]

Nature Communications
Self-amplifying mRNA vaccines use a viral replicase for self-amplification. It is currently unclear whether anti-replicase immunity occurs after saRNA vaccination, and whether such pre-existing anti-replicase immunity impacts the efficacy of subsequent ...
Xiaole Cui, Laura Amelinck, Oriane Gillon, João Paulo Portela Catani, Ya Gao, Qing Sun, Elisabeth De Sutter, Pieter Vervaeke, Janne Snoeck, Stefan Lienenklaus, Xavier Saelens, Zifu Zhong, Niek N. Sanders   +12 more
doaj   +2 more sources

Impact of B18R-Encoding Messenger Ribonucleic Acid Co-Delivery on Neutralizing Antibody Production in Self-Amplifying Messenger Ribonucleic Acid Vaccines [PDF]

Vaccines
Objectives: The COVID-19 pandemic has brought mRNA vaccines to the forefront due to their widespread use. In this study, we explored the potential advantages of the self-amplifying mRNA (saRNA) vaccine over conventional mRNA vaccines. Methods: Initially,
Yutao Wang, Lei Li, Min Liang, Gan Liu, Yinying Lu   +4 more
doaj   +2 more sources

A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants [PDF]

npj Vaccines
Pseudorabies virus (PRV) causes an acute febrile infectious disease of pigs. Since 2011, PRV variants have appeared and spread nationwide in China. mRNA vaccines present a safe alternative and can stimulate humoral and cellular immunity. We constructed a
Tong Ling, Zhang Xin, Li Huan-huan, Zheng Ya-ting, Liu Ya-mei, Guo Rong-li, Armando Mario Damiani, Chen Sai-sai, Zhang Chuan-jian, Duan Rui, Mao Shu-yu, Yu Jia-li, Zhang Qian-qian, Tao Ruo-nan, Nikolaus Osterrieder, Xia Shu-hua, Wang Ji-chun   +16 more
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Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity [PDF]

FEBS Open Bio
Self‐amplifying RNA (saRNA) vectors have garnered significant attention for their potential in transient recombinant protein expression and vaccination strategies.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
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Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity [PDF]

eLife
Self-amplifying RNA (saRNA) holds promise for durable therapeutic gene expression, but its broader utility beyond vaccines is limited by potent innate immune responses triggered during replication.
Tony KY Lim, Anne Ritoux, Luke W Paine, Larissa Ferguson, Tawab Abdul, Laura J Grundy, Ewan St John Smith   +6 more
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Divergent Delivery and Expression Kinetics of Lipid and Polymeric Nanoparticles across mRNA Modalities [PDF]

Advanced Science
Messenger ribonucleic acid (mRNA)‐based therapies, including conventional linear mRNA (linRNA), circular RNA (circRNA), and self‐amplifying RNA (saRNA), are being developed not only for vaccination but also for protein replacement, gene editing, and ...
Irafasha C. Casmil, Josh J. Friesen, Nuthan V. Bathula, Anneke Strumpel, Chia Hao Ho, Ilana Guez, Kristen Y. S. Kong, Andrew J. Varley, Shigeki J. Miyake‐Stoner, Parinaz Aliahmad, Nathaniel S. Wang, Andrew J. Geall, Anna K. Blakney   +12 more
doaj   +2 more sources

Self-Amplifying RNA: Advantages and Challenges of a Versatile Platform for Vaccine Development [PDF]

Viruses
Self-amplifying RNA is synthetic nucleic acid engineered to replicate within cells without generating viral particles. Derived from alphavirus genomes, saRNA retains the non-structural elements essential for replication while replacing the structural ...
Thomas Vallet, Marco Vignuzzi
doaj   +2 more sources

A bivalent self-amplifying RNA vaccine against yellow fever and Zika viruses [PDF]

Frontiers in Immunology
IntroductionYellow fever (YFV) and Zika (ZIKV) viruses cause significant morbidity and mortality, despite the existence of an approved YFV vaccine and the development of multiple ZIKV vaccine candidates to date.
Peter Battisti, Matthew R. Ykema, Darshan N. Kasal, Madeleine F. Jennewein, Samuel Beaver, Abbie E. Weight, Derek Hanson, Jasneet Singh, Julie Bakken, Noah Cross, Pauline Fusco, Jacob Archer, Sierra Reed, Alana Gerhardt, Justin G. Julander, Corey Casper, Corey Casper, Corey Casper, Corey Casper, Emily A. Voigt   +19 more
doaj   +2 more sources