Results 1 to 10 of about 54,168 (197)

Influence of the administration route and dose on the expression and antibody responses of a reporter and avian influenza self-amplifying mRNA vaccine in poultry [PDF]

Veterinary Quarterly
Vaccination is routinely used in industrial poultry to control infectious diseases. Vaccines based on mRNA and self-amplifying RNA (saRNA) are approved for human use, but research on their application in poultry is limited.
Janne Snoeck, Xiaole Cui, Pieter Vervaeke, Niek N. Sanders, An Garmyn   +4 more
doaj   +2 more sources

The evaluation of novel oral vaccines based on self-amplifying RNA lipid nanparticles (saRNA LNPs), saRNA transfected Lactobacillus plantarum LNPs, and saRNA transfected Lactobacillus plantarum to neutralize SARS-CoV-2 variants alpha and delta

Scientific Reports, 2021
The aim of this study was to present and evaluate novel oral vaccines, based on self-amplifying RNA lipid nanparticles (saRNA LNPs), saRNA transfected Lactobacillus plantarum LNPs, and saRNA transfected Lactobacillus plantarum, to neutralize severe acute
Reza Keikha, Seyed Mohammad Hashemi-Shahri, Ali Jebali   +2 more
doaj   +2 more sources

Optimization of In Vitro Transcription by Design of Experiment to Achieve High Self-Amplifying RNA Integrity [PDF]

Vaccines
Background: Self-amplifying mRNA (saRNA) holds promising application prospects. However, due to the inclusion of a replicase sequence, its extended length leads to premature termination during in vitro transcription (IVT), resulting in poor product ...
Chaoying Hu, Haixin Wang, Guanxing Liu, Kelei Li, Xuanxuan Zhang, Lifang Song, Fan Gao, Xing Wu, Qian Wang, Mingchen Liu, Jianyang Liu, Zhihao Fu, Xiao Ma, Miao Xu, Qunying Mao, Zhenglun Liang, Qian He   +16 more
doaj   +2 more sources

Impact of pre-existing anti-replicase immunity on the efficacy of self-amplifying mRNA vaccines [PDF]

Nature Communications
Self-amplifying mRNA vaccines use a viral replicase for self-amplification. It is currently unclear whether anti-replicase immunity occurs after saRNA vaccination, and whether such pre-existing anti-replicase immunity impacts the efficacy of subsequent ...
Xiaole Cui, Laura Amelinck, Oriane Gillon, João Paulo Portela Catani, Ya Gao, Qing Sun, Elisabeth De Sutter, Pieter Vervaeke, Janne Snoeck, Stefan Lienenklaus, Xavier Saelens, Zifu Zhong, Niek N. Sanders   +12 more
doaj   +2 more sources

Engineered VPg saRNA achieves cap-independent, low-immunogenic and precise encoding of therapeutic proteins in vivo [PDF]

Nature Communications
Self-amplifying mRNA (saRNA) vectors hold promise for the sustained expression of mRNA vaccines in vivo. However, their inherently high immunogenicity and low-fidelity replication—stemming from the RNA viral genome’s replication mechanisms—limit their ...
Zunyong Feng, Liuxi Chu, Qiang Li, Jing Zhou, Ping Wu, Xuanbo Zhang, Yuanbo Pan, Jianhua Zou, Qun Chen, Zhiliang Xu, Liang Yan, Yanjiao Huang, Xiaokun Li, Zhouguang Wang, Xiaoyuan Chen   +14 more
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Impact of B18R-Encoding Messenger Ribonucleic Acid Co-Delivery on Neutralizing Antibody Production in Self-Amplifying Messenger Ribonucleic Acid Vaccines [PDF]

Vaccines
Objectives: The COVID-19 pandemic has brought mRNA vaccines to the forefront due to their widespread use. In this study, we explored the potential advantages of the self-amplifying mRNA (saRNA) vaccine over conventional mRNA vaccines. Methods: Initially,
Yutao Wang, Lei Li, Min Liang, Gan Liu, Yinying Lu   +4 more
doaj   +2 more sources

A Self-amplifying mRNA vaccine for infectious laryngotracheitis virus (ILTV) induces efficient protective immunity [PDF]

Poultry Science
Infectious laryngotracheitis (ILT) continues to pose a significant threat to intensive poultry farming regions worldwide. Current control strategies primarily rely on live attenuated and recombinant live ILT virus (ILTV) vaccines.
Huan-huan Li, Ling Tong, Sai-sai Chen, Zhi-xiang Bi, Gang Hu, Wan-li Chen, Jia-li Yu, Ruo-nan Tao, Teng Huang, Shu-hua Xia, Nikolaus Osterrieder, Ji-chun Wang   +11 more
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A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants [PDF]

npj Vaccines
Pseudorabies virus (PRV) causes an acute febrile infectious disease of pigs. Since 2011, PRV variants have appeared and spread nationwide in China. mRNA vaccines present a safe alternative and can stimulate humoral and cellular immunity. We constructed a
Tong Ling, Zhang Xin, Li Huan-huan, Zheng Ya-ting, Liu Ya-mei, Guo Rong-li, Armando Mario Damiani, Chen Sai-sai, Zhang Chuan-jian, Duan Rui, Mao Shu-yu, Yu Jia-li, Zhang Qian-qian, Tao Ruo-nan, Nikolaus Osterrieder, Xia Shu-hua, Wang Ji-chun   +16 more
doaj   +2 more sources

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity [PDF]

FEBS Open Bio
Self‐amplifying RNA (saRNA) vectors have garnered significant attention for their potential in transient recombinant protein expression and vaccination strategies.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
doaj   +2 more sources

Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity [PDF]

eLife
Self-amplifying RNA (saRNA) holds promise for durable therapeutic gene expression, but its broader utility beyond vaccines is limited by potent innate immune responses triggered during replication.
Tony KY Lim, Anne Ritoux, Luke W Paine, Larissa Ferguson, Tawab Abdul, Laura J Grundy, Ewan St John Smith   +6 more
doaj   +2 more sources