Results 71 to 80 of about 99,522,423 (293)

Evaluating SARS-CoV-2 Spike Protein Transfection in HEK-293T Cells for VLP Applications

Journal of Experimental Life Science
The spike protein in SARS-CoV-2 works mainly to initiate infections, especially for binding to host cells and fusing with cell membranes. Characterizing SARS-CoV-2 spikes protein transfection cells could optimize the production of VLP in HEK-293T cells ...
Ilmiana Nurur Rohmah, Kavana Hafil Kusuma, Yuyun Ika Christina, Dinia Rizqi Dwijayanti, Dewi Mustikaningtyas, Nashi Widodo, Muhammad Sasmito Djati   +6 more
doaj   +1 more source

SARS-CoV-2 variants – Evolution, spike protein, and vaccines

Biomedical Journal, 2022
Despite the rising natural and vaccines mediated immunity, several countries have experienced a resurgence of the Coronavirus disease of 2019 (COVID-19) due to the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. From Alpha to Omicron, the variants of concern (VOC) have evolved several spike protein mutations that may
Kai-Wei K. Chen, Daniel Tsung-Ning Huang, Li-Min Huang   +2 more
openaire   +3 more sources

Avoiding culture shock with the SARS-CoV-2 spike protein

eLife, 2021
When culturing SARS-CoV-2 in the laboratory it is vital to avoid deletions in the gene for the spike protein that could affect the interpretation of experiments.
openaire   +3 more sources

Sea Urchin Pigments as Potential Therapeutic Agents Against the Spike Protein of SARS-CoV-2 Based on in Silico Analysis [PDF]

, 2020
Several studies have been published regarding the interaction between the spike protein of the novel coronavirus SARS-CoV-2 and ACE2 receptor in the host cells.
Jimena Pía, Fernandez, Ayelén, Gázquez, Mercedes, Vera-Piombo, Elena Susana, Barbieri, Florencia, Chaar, Marisa, Avaro, Agustín, Gittardi, Lucas, Sepulveda, Tamara, Rubilar, Erina Noé, Seiler   +9 more
core   +5 more sources

A Single-Component Luminescent Biosensor for the SARS-CoV-2 Spike Protein

Journal of the American Chemical Society, 2022
Abstract Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of E ...
Matthew Ravalin, Heegwang Roh, Rahul Suryawanshi, G. Renuka Kumar, John Pak, Melanie Ott, Alice Y. Ting   +6 more
openaire   +3 more sources

Evolution of increased positive charge on the SARS-CoV-2 spike protein may be adaptation to human transmission

iScience, 2023
Summary: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve and infect individuals. The exterior surface of the SARS-CoV-2 virion is dominated by the spike protein, and the current work examined spike protein biochemical
Matthew Cotten, My V.T. Phan
doaj   +1 more source

Adsorption of pulmonary and exogeneous surfactants on SARS-CoV-2 spike protein

Journal of Colloid and Interface Science, 2022
ABSTRACT COVID-19 is transmitted by inhaling SARS-CoV-2 virions, which are enveloped by a lipid bilayer decorated by a “crown” of Spike protein protrusions. In the respiratory tract, virions interact with surfactant films composed of phospholipids and cholesterol that coat lung airways. Here, we explore by using coarse-grained molecular
Santo, Kolattukudy P., Neimark, Alexander V.   +1 more
openaire   +3 more sources

Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein [PDF]

ACS Central Science, 2020
The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date. Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the ...
Lorenzo Casalino, Zied Gaieb, Jory A. Goldsmith, Christy K. Hjorth, Abigail C. Dommer, Aoife M. Harbison, Carl A. Fogarty, Emilia P. Barros, Bryn C. Taylor, Jason S. McLellan, Elisa Fadda, Rommie E. Amaro   +11 more
openaire   +5 more sources

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

T cell epitopes of SARS-CoV-2 spike protein and conserved surface protein of Plasmodium malariae share sequence homology

Open Life Sciences, 2021
Since its emergence in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading remarkably fast worldwide. Effective countermeasures require the rapid development of data and tools to monitor its spread and better ...
Hassan Md. Mehedi, Sharmin Shirina, Hong Jinny, Lee Hoi-Seon, Kim Hyeon-Jin, Hong Seong-Tshool   +5 more
doaj   +1 more source