Results 1 to 10 of about 312 (58)
A novel and safe SmartCap® SC101 to develop the COVID-19 mRNA vaccine STP2104 inducing potent immune responses in humans [PDF]
Frontiers in ImmunologyWe have developed a 5′-capping library screening (CLS) method using over 30 different novel cap analogues. The optimal 5′-cap for the coronavirus disease 2019 (COVID-19) mRNA vaccine STP2104 was selected and applied.
Rachel Kim +23 more
doaj +4 more sources
Screening and Identification of Trichoderma Strains isolated from Natural Habitats in China with Potential Agricultural Applications. [PDF]
Biomed Res Int, 2021 Trichoderma spp. are widely distributed in natural habitats and have been evaluated as a potential biocontrol agent (BCA) for disease control and plant growth promotion. In this study, 1308 Trichoderma strains were obtained from the plant rhizosphere soil, above‐ground plants, and decaying wood from natural habitats in China. Among them, 49 Trichoderma
Xue M +9 more
europepmc +2 more sources
Discovery and development of a safe and efficient COVID-19 mRNA vaccine, STP2104, using a novel capping library screening method [PDF]
Frontiers in ImmunologyMessenger RNA (mRNA) vaccines represent a critical avenue for coronavirus disease 2019 (COVID-19) prevention. We developed a COVID-19 mRNA vaccine encoding a codon-optimized full-length ancestral spike (S) protein with a signal peptide, which employs our
Kanghyun Choi +36 more
doaj +2 more sources
Pathway Evolution Through a Bottlenecking-Debottlenecking Strategy and Machine Learning-Aided Flux Balancing. [PDF]
Adv Sci (Weinh)A biofoundry‐assisted strategy for pathway bottlenecking and debottlenecking enables the parallel evolution of all pathway enzymes along a predictable evolutionary trajectory. A machine learning model can further relax the epistasis of the evolved pathway by optimizing the corresponding promoter combinations.
Deng H +13 more
europepmc +2 more sources
The serine transporter SdaC prevents cell lysis upon glucose depletion in Escherichia coli. [PDF]
Microbiologyopen, 2020 Our results reveal that the serine transporter SdaC plays a critical role in maintaining amino acid homeostasis during shifts in nutrient availability in Escherichia coli. Abstract The amino acid serine plays diverse metabolic roles, yet bacteria actively degrade exogenously provided serine via deamination to pyruvate.
Kriner MA, Subramaniam AR.
europepmc +2 more sources
Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides. [PDF]
Microb BiotechnolEscherichia coli W demonstrates enhanced flavonoid tolerance while the optimisation of sucrose metabolism in this strain enables efficient chemical glycosylation. Elevated UDP‐glucose production requires both pathway overexpression and removal of native glucose catabolism.
Carranza-Saavedra D +8 more
europepmc +2 more sources
Nitrogen Fertilizer Recommendations for Sugarcane Production for Sugar on Florida Sand Soils
EDIS, 2016 Sands used in sugarcane production in Florida have low levels of organic matter, silt, and clay, and they provide little nitrogen (N) through mineralization of organic matter and possess a low capacity for N retention as a result.
J. Mabry McCray +2 more
doaj +5 more sources
Advanced Electronic Materials, Volume 9, Issue 3, March 2023., 2023 Pd NPs‐decorated ultrathin SnO2 film for hydrogen gas detection at low temperature is prepared on PI. This kind of flexible film hydrogen sensor exhibits superb sensitivity and wide detection range. The combination of atomic layer deposition technology (ALD) and cluster beam deposition technology (CBD) provides a feasible strategy for manufacturing ...
Qiang Ren +9 more
wiley +1 more source
Engineered acetoacetate‐inducible whole‐cell biosensors based on the AtoSC two‐component system
Biotechnology and Bioengineering, Volume 118, Issue 11, Page 4278-4289, November 2021., 2021 Rutter and coworkers engineer a range of acetoacetate sensitive whole‐cell biosensors, based on the AtoSC two‐component system. A modelling and sensitivity analysis approach is applied to identify factors that are important in determining the response of these biosensors.
Jack W. Rutter +9 more
wiley +1 more source
MicrobiologyOpen, Volume 9, Issue 8, August 2020., 2020 Previous work on antibiotic‐tolerant persisters of pathogenic Escherichia coli highlighted the role of PasT, described as a toxin of a toxin–antitoxin system, in their resilience. Here, we show that PasT is not a toxin but rather the bacterial homolog of mitochondrial Coq10 that guides the electron carrier ubiquinone in respiratory electron transport ...
Cinzia Fino +5 more
wiley +1 more source