Results 11 to 20 of about 306 (57)
Pathway Evolution Through a Bottlenecking-Debottlenecking Strategy and Machine Learning-Aided Flux Balancing. [PDF]
Adv Sci (Weinh)A biofoundry‐assisted strategy for pathway bottlenecking and debottlenecking enables the parallel evolution of all pathway enzymes along a predictable evolutionary trajectory. A machine learning model can further relax the epistasis of the evolved pathway by optimizing the corresponding promoter combinations.
Deng H +13 more
europepmc +2 more sources
The serine transporter SdaC prevents cell lysis upon glucose depletion in Escherichia coli. [PDF]
Microbiologyopen, 2020 Our results reveal that the serine transporter SdaC plays a critical role in maintaining amino acid homeostasis during shifts in nutrient availability in Escherichia coli. Abstract The amino acid serine plays diverse metabolic roles, yet bacteria actively degrade exogenously provided serine via deamination to pyruvate.
Kriner MA, Subramaniam AR.
europepmc +2 more sources
Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides. [PDF]
Microb BiotechnolEscherichia coli W demonstrates enhanced flavonoid tolerance while the optimisation of sucrose metabolism in this strain enables efficient chemical glycosylation. Elevated UDP‐glucose production requires both pathway overexpression and removal of native glucose catabolism.
Carranza-Saavedra D +8 more
europepmc +2 more sources
Advanced Electronic Materials, Volume 9, Issue 3, March 2023., 2023 Pd NPs‐decorated ultrathin SnO2 film for hydrogen gas detection at low temperature is prepared on PI. This kind of flexible film hydrogen sensor exhibits superb sensitivity and wide detection range. The combination of atomic layer deposition technology (ALD) and cluster beam deposition technology (CBD) provides a feasible strategy for manufacturing ...
Qiang Ren +9 more
wiley +1 more source
Engineered acetoacetate‐inducible whole‐cell biosensors based on the AtoSC two‐component system
Biotechnology and Bioengineering, Volume 118, Issue 11, Page 4278-4289, November 2021., 2021 Rutter and coworkers engineer a range of acetoacetate sensitive whole‐cell biosensors, based on the AtoSC two‐component system. A modelling and sensitivity analysis approach is applied to identify factors that are important in determining the response of these biosensors.
Jack W. Rutter +9 more
wiley +1 more source
MicrobiologyOpen, Volume 9, Issue 8, August 2020., 2020 Previous work on antibiotic‐tolerant persisters of pathogenic Escherichia coli highlighted the role of PasT, described as a toxin of a toxin–antitoxin system, in their resilience. Here, we show that PasT is not a toxin but rather the bacterial homolog of mitochondrial Coq10 that guides the electron carrier ubiquinone in respiratory electron transport ...
Cinzia Fino +5 more
wiley +1 more source
Measuring Colloidal Forces With Atomic Force Microscopy 1: Salt Influence on Hydrophobic and Hydrophilic Interactions. [PDF]
Microsc Res TechColloidal forces play an important role in many life phenomena. Here, we present the preparation of hydrophobic and hydrophilic systems for the study of colloidal forces by AFM. The data are modeled using an extended DLVO model. ABSTRACT Colloidal forces are essential for maintaining the stability and functionality of colloidal systems, affecting ...
Ponce-Gonzalez LN +2 more
europepmc +2 more sources
Stem Cells International, Volume 2019, Issue 1, 2019., 2019 The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human‐specific disease processes. We previously have identified an evolutionary young miRNA, miR‐1290, to be essential in neural stem cell proliferation and neuronal differentiation.
Dalia Moore +11 more
wiley +1 more source
Rational Design of Lipid Nanoparticles for Enhanced mRNA Vaccine Delivery via Machine Learning
Small, Volume 21, Issue 8, February 25, 2025.This study applies machine learning (ML) to identify ionizable lipids for enhanced mRNA delivery. A random forest regression model is used to analyze lipid nanoparticles (LNPs) and predict mRNA expression efficiency based on features regarding chemical substructure and LNP composition.
Seo‐Hyeon Bae +27 more
wiley +1 more source
Biosynthesis of natural and halogenated plant monoterpene indole alkaloids in yeast. [PDF]
Nat Chem Biol, 2023 Bradley SA +31 more
europepmc +1 more source